scholarly journals Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen

2016 ◽  
Vol 78 (2) ◽  
pp. 309-311 ◽  
Author(s):  
Hiroshi BANNAI ◽  
Manabu NEMOTO ◽  
Koji TSUJIMURA ◽  
Takashi YAMANAKA ◽  
Ken MAEDA ◽  
...  
1997 ◽  
Vol 8 (3) ◽  
pp. 57-61 ◽  
Author(s):  
Takeo SUGIURA ◽  
Takashi KONDO ◽  
Tomio MATSUMURA ◽  
Hiroshi IMAGAWA ◽  
Masanobu KAMADA ◽  
...  

2005 ◽  
Vol 12 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Ken Maeda ◽  
Fuminori Mizukoshi ◽  
Masataka Hamano ◽  
Kazushige Kai ◽  
Takashi Kondo ◽  
...  

ABSTRACT Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNP V YSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNP V YSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.


Vaccine ◽  
2008 ◽  
Vol 26 (19) ◽  
pp. 2335-2343 ◽  
Author(s):  
Cristina Rosas ◽  
Gerlinde R. Van de Walle ◽  
Stephan M. Metzger ◽  
Karin Hoelzer ◽  
Edward J. Dubovi ◽  
...  

2017 ◽  
Vol 51 ◽  
pp. 46-53
Author(s):  
Maksat Akhmedzhanov ◽  
Rysbek Nurgaziev ◽  
Jailobek Orozov ◽  
Irmgard Moser ◽  
Nikolaus Osterrieder ◽  
...  

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