scholarly journals Interaction of bacteriophage T4 with reconstituted cell envelopes of Escherichia coli K-12.

1979 ◽  
Vol 140 (3) ◽  
pp. 1071-1080 ◽  
Author(s):  
H Furukawa ◽  
H Yamada ◽  
S Mizushima
2001 ◽  
Vol 47 (7) ◽  
pp. 680-684 ◽  
Author(s):  
Brian D Corbin ◽  
Robert JC McLean ◽  
Gary M Aron

An Escherichia coli K-12 biofilm was grown at a dilution rate of 0.028 h-1 for 48 h in a glucose-limited chemostat coupled to a modified Robbins' device to determine its susceptibility to infection by bacteriophage T4. Bacteriophage T4 at a multiplicity of infection (MOI) of 10 caused a log reduction in biofilm density (expressed as colony forming units (CFU) per cm2) at 90 min postinfection. After 6 h, a net decrease and equilibrium in viral titer was seen. When biofilms were exposed to T4 phage at a MOI of 100, viral titer doubled after 90 min. After 6 h, viral titers (expressed as plaque forming units (PFU) per cm2) stabilized at levels approximately one order of magnitude higher than seen at a MOI of 10. Scanning confocal laser microscopy images also indicated disruption of biofilm morphology following T4 infection with the effects being more pronounced at a MOI of 100 than at a MOI of 10. These results imply that biofilms under carbon limitation can act as natural reservoirs for bacteriophage and that bacteriophage can have some influence on biofilm morphology.Key words: bacteriophage T4, biofilm, biofilm morphology, bacteriophage ecology, carbon limitation.


1978 ◽  
Vol 24 (6) ◽  
pp. 761-764 ◽  
Author(s):  
Edward E. Ishiguro ◽  
William D. Ramey

The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA− derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA− strains. Treatment with chloramphenicol (CAM) did not alter the size distributions of either rel+ or relA− strains except when they were rel+dap−. CAM treatment of rel+dap− strains resulted in an increase in cell size. It is proposed that these results reflect differences in the structures of the cell envelopes of rel+ and relA− bacteria.


Genetics ◽  
1986 ◽  
Vol 114 (3) ◽  
pp. 705-716
Author(s):  
Muriel B Herrington ◽  
Anjali Kohli ◽  
Maria Faraci

ABSTRACT We have extended our previous study on the suppression of frameshift mutants by Escherichia coli thyA mutants by assaying suppression of 15 rIIB frameshift mutants of bacteriophage T4 on one of our suppressing thyA mutant strains. The majority of insertion mutants were suppressible, whereas none of the deletion mutants tested was suppressible. Frameshift suppression could be inhibited by adding thymidine to the assay medium, but was not affected by the presence of a restrictive rpsL mutation in the host strain. We suggest that the frameshift suppression event occurs at a nonsense codon generated by the frameshift mutation.


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