scholarly journals Characterization of Mycobacterium tuberculosis Rv3676 (CRPMt), a Cyclic AMP Receptor Protein-Like DNA Binding Protein

2005 ◽  
Vol 187 (22) ◽  
pp. 7795-7804 ◽  
Author(s):  
Guangchun Bai ◽  
Lee Ann McCue ◽  
Kathleen A. McDonough
Keyword(s):  
Transcription Factor ◽  
Mycobacterium Tuberculosis ◽  
Cyclic Amp ◽  
Receptor Protein ◽  
Binding Motif ◽  
Promoter Regions ◽  
Motif Model ◽  
Dna Motif ◽  
Camp Receptor

ABSTRACT Little is known about cyclic AMP (cAMP) function in Mycobacterium tuberculosis, despite its ability to encode 15 adenylate cyclases and 10 cNMP-binding proteins. M. tuberculosis Rv3676, which we have designated CRPMt, is predicted to be a cAMP-dependent transcription factor. In this study, we characterized CRPMt's interactions with DNA and cAMP, using experimental and computational approaches. We used Gibbs sampling to define a CRPMt DNA motif that resembles the cAMP receptor protein (CRP) binding motif model for Escherichia coli. CRPMt binding sites were identified in a total of 73 promoter regions regulating 114 genes in the M. tuberculosis genome, which are being explored as a regulon. Specific CRPMt binding caused DNA bending, and the substitution of highly conserved nucleotides in the binding site resulted in a complete loss of binding to CRPMt. cAMP enhanced CRPMt's ability to bind DNA and caused allosteric alterations in CRPMt conformation. These results provide the first direct evidence for cAMP binding to a transcription factor in M. tuberculosis, suggesting a role for cAMP signal transduction in M. tuberculosis and implicating CRPMt as a cAMP-responsive global regulator.

scholarly journals Identification and Characterization of a Novel cAMP Receptor Protein in the CyanobacteriumSynechocystissp. PCC 6803

2000 ◽  
Vol 275 (9) ◽  
pp. 6241-6245 ◽  
Author(s):  
Hidehisa Yoshimura ◽  
Toru Hisabori ◽  
Shuichi Yanagisawa ◽  
Masayuki Ohmori
Keyword(s):  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Camp Receptor ◽  
Identification And Characterization ◽  
Pcc 6803

scholarly journals Regulation of the expression of whiB1 in Mycobacterium tuberculosis: role of cAMP receptor protein

Microbiology ◽  
2006 ◽  
Vol 152 (9) ◽  
pp. 2749-2756 ◽  
Author(s):  
Nisheeth Agarwal ◽  
Tirumalai R. Raghunand ◽  
William R. Bishai
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Binding Site ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Mobility Shift ◽  
Camp Analogue ◽  
Camp Receptor ◽  
Fusion Analysis ◽  
Electrophoretic Mobility Shift Assays

The wbl (whiB-like) genes encode putative transcription factors unique to actinomycetes. This study characterized the promoter element of one of the seven wbl genes of Mycobacterium tuberculosis, whiB1 (Rv3219c). The results reveal that whiB1 is transcribed by a class I-type cAMP receptor protein (CRP)-dependent promoter, harbouring a CRP-binding site positioned at −58.5 with respect to its transcription start point. In vivo promoter activity analysis and electrophoretic mobility shift assays suggest that the expression of whiB1 is indeed regulated by cAMP-dependent binding of CRPM (encoded by the M. tuberculosis gene Rv3676) to the whiB1 5′ untranslated region (5′UTR). β-Galactosidase gene fusion analysis revealed induction of the whiB1 promoter in M. tuberculosis on addition of exogenous dibutyric cAMP (a diffusible cAMP analogue) only when an intact CRP-binding site was present. These results indicate that M. tuberculosis whiB1 transcription is regulated in part by cAMP levels via direct binding of cAMP-activated CRPM to a consensus CRP-binding site in the whiB1 5′UTR.

scholarly journals The cyclic AMP (cAMP)-cAMP receptor protein complex functions both as an activator and as a corepressor at the tsx-p2 promoter of Escherichia coli K-12.

1991 ◽  
Vol 173 (17) ◽  
pp. 5419-5430 ◽  
Author(s):  
P Gerlach ◽  
L Søgaard-Andersen ◽  
H Pedersen ◽  
J Martinussen ◽  
P Valentin-Hansen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Protein Complex ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Complex Functions ◽  
P2 Promoter ◽  
Camp Receptor ◽  
K 12

Random mutagenesis of global transcription factor cAMP receptor protein for improved osmotolerance

2011 ◽  
Vol 109 (5) ◽  
pp. 1165-1172 ◽  
Author(s):  
Hongfang Zhang ◽  
Huiqing Chong ◽  
Chi Bun Ching ◽  
Rongrong Jiang
Keyword(s):  
Transcription Factor ◽  
Random Mutagenesis ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Camp Receptor

scholarly journals Deletion of a Cyclic AMP Receptor Protein Homologue Diminishes Germination and Affects Morphological Development of Streptomyces coelicolor

2004 ◽  
Vol 186 (6) ◽  
pp. 1893-1897 ◽  
Author(s):  
A. Derouaux ◽  
S. Halici ◽  
H. Nothaft ◽  
T. Neutelings ◽  
G. Moutzourelis ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Streptomyces Coelicolor ◽  
Regulatory Proteins ◽  
Receptor Protein ◽  
Growth Delay ◽  
Morphological Development ◽  
Camp Receptor Protein ◽  
Reading Frame ◽  
Physiological Processes ◽  
Camp Receptor

ABSTRACT Open reading frame SCO3571 of Streptomyces coelicolor encodes a protein of the cyclic AMP (cAMP) receptor protein (CRP) superfamily of regulatory proteins. A mutant revealed a dramatic defect in germination, followed by growth delay and earlier sporulation. This phenotype correlates with those of an adenylate cyclase (cya) mutant that cannot synthesize cAMP. This finding suggests that S. coelicolor may use a Cya-cAMP-CRP system to trigger complex physiological processes such as morphogenesis.

scholarly journals Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA

Microbiology ◽  
2006 ◽  
Vol 152 (8) ◽  
pp. 2207-2219 ◽  
Author(s):  
Byung-Kwan Cho ◽  
Eric M. Knight ◽  
Bernhard Ø. Palsson
Keyword(s):  
Escherichia Coli ◽  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Expression Profiles ◽  
Direct Interaction ◽  
Anaerobic Growth ◽  
Binding Motif ◽  
Promoter Regions ◽  
Chip Analysis

ArcA is a global transcription factor required for optimal growth of Escherichia coli during anaerobic growth. In this study, the role of ArcA on the transcriptional regulatory subnetwork of the fad regulon was investigated. Gene expression profiles of deletion mutants (ΔarcA, ΔfadR and ΔarcA/ΔfadR) indicated that (i) ArcA is a major transcription factor for the transcriptional regulation of fatty acid metabolism in the absence of oxygen, and (ii) ArcA and FadR cooperatively regulate the fad regulon under anaerobic conditions. To determine the direct interaction between ArcA and the promoters of the fad regulon genes, chromatin immunoprecipitation (ChIP) analysis was performed. ChIP analysis suggested that ArcA directly binds to the promoter regions of the fad regulon genes in vivo. An ArcA-binding motif was identified from known binding sequences and predicted putative binding sites in the promoter regions of the fad regulon genes. These results indicate that ArcA directly represses the expression of fad regulon genes during anaerobic growth.

scholarly journals Cyclic AMP (cAMP) and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia coli

2005 ◽  
Vol 187 (6) ◽  
pp. 2066-2076 ◽  
Author(s):  
Liang Wang ◽  
Yoshifumi Hashimoto ◽  
Chen-Yu Tsao ◽  
James J. Valdes ◽  
William E. Bentley
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Carbon Sources ◽  
Cell Communication ◽  
Receptor Protein ◽  
Upstream Region ◽  
E Coli ◽  
Autoinducer 2 ◽  
Serovar Typhimurium ◽  
Camp Receptor

ABSTRACT Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for “luxS regulated”) operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes.

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