scholarly journals A Lipase Isolated from the Silkworm Bombyx mori Shows Antiviral Activity against Nucleopolyhedrovirus

2003 ◽  
Vol 77 (19) ◽  
pp. 10725-10729 ◽  
Author(s):  
Kangayam M. Ponnuvel ◽  
Hiroshi Nakazawa ◽  
Seiichi Furukawa ◽  
Ai Asaoka ◽  
Jun Ishibashi ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Antiviral Activity ◽  
Bombyx Mori ◽  
Northern Blot Analysis ◽  
Digestive Enzyme ◽  
Homology Search ◽  
Digestive Juice ◽  
Strong Antiviral Activity ◽  
Silkworm Bombyx Mori

ABSTRACT A protein showing strong antiviral activity against Bombyx mori nucleopolyhedrovirus (BmNPV) was purified from the digestive juice of B. mori larvae. A homology search of the deduced amino acid sequence of the protein cDNA revealed 56% homology with Drosophila melanogaster lipase and 21% homology with human lipase. As lipase activity of the protein was confirmed in vitro, this protein was designated Bmlipase-1. Northern blot analysis showed that the Bmlipase-1 gene is expressed in the midgut but not in other tissues, nor is it activated by BmNPV infection. In addition, the Bmlipase-1 gene was shown not to be expressed in the molting and wandering stages, indicating that the gene is hormonally regulated. Our results suggest that an insect digestive enzyme has potential as a physiological barrier against BmNPV at the initial site of viral infection.

IN VITRO ANTIVIRAL ACTIVITY OF AN ALKALINE TRYPSIN FROM THE DIGESTIVE JUICE OF Bombyx mori LARVAE AGAINST NUCLEOPOLYHEDROVIRUS

2012 ◽  
Vol 81 (2) ◽  
pp. 90-104 ◽  
Author(s):  
Kangayam M. Ponnuvel ◽  
Krishnappa Nithya ◽  
Sasibhushan Sirigineedi ◽  
Arvind K. Awasthi ◽  
Minoru Yamakawa
Keyword(s):  
Antiviral Activity ◽  
Bombyx Mori ◽  
Digestive Juice

scholarly journals Vitellogenin receptor selectively endocytoses female-specific and highly-expressed hemolymph proteins in the silkworm, Bombyx mori

2017 ◽  
Vol 95 (4) ◽  
pp. 510-516 ◽  
Author(s):  
Chaoshan Han ◽  
Enxiang Chen ◽  
Guanwang Shen ◽  
Zhixin Peng ◽  
Yinying Xu ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bombyx Mori ◽  
Sf9 Cells ◽  
Tissue Localization ◽  
Vitellogenin Receptor ◽  
Hemolymph Proteins ◽  
Female Specific ◽  
Oocyte Membrane ◽  
Silkworm Bombyx Mori

VgR, a member of the LDLR family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, BmVgR has been shown to interact with BmSP1 in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with BmVg and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells. Parallel immunofluorescence showed that most BmVg and BmVgR were localized in the inner oocyte membrane, showing tissue localization similar to that of BmVg labeled with pHrodo Red absorbed by the ovaries on day 2 of pupation. Although BmSP1 showed localization similar to BmVgR during the same phase, little BmSP1 was present in the ovary. Additionally, BmSP1 did not exist in ovaries when the ovaries contained BmVgR on day 5 of pupation, suggesting that BmSP1 in the ovaries was not endocytosed by BmVgR. In summary, BmVgR could facilitate uptake of BmVg by developing oocytes, but did not modulate in the transport of BmSP1.

In vitro action of prothoracicotropic hormone on RNA-synthesis in the prothoracic gland of the silkworm, Bombyx mori

1986 ◽  
Vol 32 (8) ◽  
pp. 711-717 ◽  
Author(s):  
Yasuo Aizono ◽  
Noboru Matsuo ◽  
Yoshihiro Yoshida ◽  
Gunki Funatsu ◽  
Masaru Funatsu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Rna Synthesis ◽  
Prothoracic Gland ◽  
Prothoracicotropic Hormone ◽  
Silkworm Bombyx Mori

In vitro translation of RNA from the midgut of the silkworm, Bombyx mori, infected with a densonucleosis virus

1988 ◽  
Vol 52 (2) ◽  
pp. 259-267 ◽  
Author(s):  
Michihiro Kobayashi ◽  
Yoshifumi Hashimoto ◽  
Hiroo Seki ◽  
Hitoshi Watanabe
Keyword(s):  
Bombyx Mori ◽  
In Vitro Translation ◽  
Silkworm Bombyx Mori

Antiviral activity in the mulberry silkworm, Bombyx mori L.

2006 ◽  
Vol 7 (S2) ◽  
pp. 350-356 ◽  
Author(s):  
Hui-peng Yao ◽  
Xiao-feng Wu ◽  
K. Gokulamma
Keyword(s):  
Antiviral Activity ◽  
Bombyx Mori ◽  
Bombyx Mori L ◽  
Mulberry Silkworm ◽  
Silkworm Bombyx Mori
Sign in / Sign up

Export Citation Format

Share Document