Determination of Free and Esterified Fatty Acids in Hydrocoles of Different Content of Polyunsaturated Fatty Acids by Gas–liquid Chromatography

2020 ◽  
Vol 75 (10) ◽  
pp. 1310-1321
Author(s):  
A. A. Nikonova ◽  
S. M. Shishlyannikov ◽  
T. A. Shishlyannikova ◽  
T. N. Avezova ◽  
T. A. Babenko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Polyunsaturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Esterified Fatty Acids ◽  
Different Content

A Comparison of Gas-Liquid Chromatography and a Titration Method for the Determination of Plasma Non-Esterified Fatty Acid Levels during Pregnancy

1975 ◽  
Vol 12 (1-6) ◽  
pp. 249-251 ◽  
Author(s):  
Maureen Young ◽  
Elizabeth A. Gilmore ◽  
R. G. McDonald-Gibson ◽  
Jennifer A. Elliott
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Post Partum ◽  
Gas Liquid Chromatography ◽  
Plasma Nefa ◽  
Titration Method ◽  
Esterified Fatty Acids ◽  
Nefa Level

The level of plasma non-esterified fatty acids (NEFA) was measured by gas-liquid chromatography (GLC) and a titration method in 194 samples collected during pregnancy and from four days to 24 weeks post partum. Both techniques indicated a similar pattern of changes in plasma NEFA associated with pregnancy. The titration estimates of NEFA level were usually greater than those measured by GLC, and there was some suggestion that the disparity between the methods was increased at the end of pregnancy and was reduced at six weeks after delivery.

Ozonolysis of unsaturated fatty acids. II. Esterification of the total products from the oxidative decomposition of ozonides with 2,2-dimethoxypropane

1967 ◽  
Vol 45 (13) ◽  
pp. 1405-1410 ◽  
Author(s):  
John D. Castell ◽  
R. G. Ackman
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Unsaturated Fatty Acids ◽  
Performic Acid ◽  
Acid Oxidation ◽  
Gas Liquid Chromatography ◽  
Positional Isomers ◽  
Flame Ionization ◽  
Hydrogen Flame

The total acidic products from the performic acid oxidation of the ozonide of methyl oleate formed in methanol may be esterified directly in a few hours with 2,2-dimethoxypropane. The ester concentrations are adequate for the determination of the positional isomers of monoethylenic fatty acids directly from the reaction mixture, using a hydrogen flame ionization gas–liquid chromatography detector. Dimethyl sulfoxide was not required to prevent the breakdown of 2,2-dimethoxypropane under the conditions employed.

A 57Co-soap assay for plasma total non-esterified fatty acids compared with a gas-liquid chromatographic method.

1980 ◽  
Vol 26 (13) ◽  
pp. 1879-1882 ◽  
Author(s):  
D C Turnell ◽  
C P Price ◽  
M M France
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Chromatographic Method ◽  
Gas Liquid Chromatography ◽  
Esterified Fatty Acids ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract We describe a relatively rapid radiochemical assay for determining plasma non-esterified fatty acids, by use of a Dole-type (J. Clin. Invest. 35: 150, 1956) extraction and formation of 57Co soaps. Results correlated well (r = 0.957) with those by gas-liquid chromatography. The between-batch CV for 1.12 mmol of non-esterified fatty acids per liter was 7%; the sensitivity is estimated to be 22 mumol/L.

A method for the quantitative determination of individual free fatty acids in milk by ion exchange resin adsorption and gas-liquid chromatography

1983 ◽  
Vol 50 (3) ◽  
pp. 321-329 ◽  
Author(s):  
Eric C. Needs ◽  
Graeme D. Ford ◽  
A. Jane Owen ◽  
Brian Tuckley ◽  
Malcolm Anderson
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Free Fatty Acids ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Gas Liquid Chromatography ◽  
Internal Standards ◽  
Resin Adsorption ◽  
The Individual

SummaryA quantitative method for rapid routine analysis of individual free fatty acids (FFA) in milk was developed. Lipid was extracted from milk in ether and FFA were recovered by shaking the extract with anion exchange resin Amberlyst 26. The resin-bound FFA were methylated directly and the individual acids quantified, using internal standards, by gas-liquid chromatography. The properties of the resin were measured. The validity of the method was established by extraction of FFA mixtures and milk. Individual acids were, on average, found to be within 6% of the actual concentration present in the mixture. An average coefficient of variation of 4·3% was achieved for the major individual fatty acids on repeated extraction of a single milk sample.

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