A Comparison of Gas-Liquid Chromatography and a Titration Method for the Determination of Plasma Non-Esterified Fatty Acid Levels during Pregnancy

1975 ◽  
Vol 12 (1-6) ◽  
pp. 249-251 ◽  
Author(s):  
Maureen Young ◽  
Elizabeth A. Gilmore ◽  
R. G. McDonald-Gibson ◽  
Jennifer A. Elliott
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Post Partum ◽  
Gas Liquid Chromatography ◽  
Plasma Nefa ◽  
Titration Method ◽  
Esterified Fatty Acids ◽  
Nefa Level

The level of plasma non-esterified fatty acids (NEFA) was measured by gas-liquid chromatography (GLC) and a titration method in 194 samples collected during pregnancy and from four days to 24 weeks post partum. Both techniques indicated a similar pattern of changes in plasma NEFA associated with pregnancy. The titration estimates of NEFA level were usually greater than those measured by GLC, and there was some suggestion that the disparity between the methods was increased at the end of pregnancy and was reduced at six weeks after delivery.

Determination of Erythrocyte Fatty Acids by Capillary Gas Liquid Chromatography

1987 ◽  
Vol 24 (3) ◽  
pp. 293-300 ◽  
Author(s):  
Andrew J Taylor ◽  
Harry I Pandov ◽  
Nigel Lawson
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Erythrocyte Membranes ◽  
Gas Liquid Chromatography ◽  
Fatty Acid Profiles ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Arachidonic Acids

A capillary gas liquid chromatography method was established for the routine determination of fatty acid profiles from washed erythrocyte membranes. Only the five major fatty acids found in erythrocytes (palmitic, stearic, oleic, linoleic and arachidonic acids) had acceptable precision (CVs <10·0%) for use in establishing quantitative differences between groups of individuals. Reference values were established for the relative amounts of these five fatty acids in adults. Significant alterations in the relative concentrations of oleic and arachidonic acids were found after storage at +4°C for 24 h, which has important implications in the study of changes in erythrocyte fatty acids in cancer and diabetes.

Determination of Free and Esterified Fatty Acids in Hydrocoles of Different Content of Polyunsaturated Fatty Acids by Gas–liquid Chromatography

2020 ◽  
Vol 75 (10) ◽  
pp. 1310-1321
Author(s):  
A. A. Nikonova ◽  
S. M. Shishlyannikov ◽  
T. A. Shishlyannikova ◽  
T. N. Avezova ◽  
T. A. Babenko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Polyunsaturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Esterified Fatty Acids ◽  
Different Content

Ozonolysis of unsaturated fatty acids. II. Esterification of the total products from the oxidative decomposition of ozonides with 2,2-dimethoxypropane

1967 ◽  
Vol 45 (13) ◽  
pp. 1405-1410 ◽  
Author(s):  
John D. Castell ◽  
R. G. Ackman
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Unsaturated Fatty Acids ◽  
Performic Acid ◽  
Acid Oxidation ◽  
Gas Liquid Chromatography ◽  
Positional Isomers ◽  
Flame Ionization ◽  
Hydrogen Flame

The total acidic products from the performic acid oxidation of the ozonide of methyl oleate formed in methanol may be esterified directly in a few hours with 2,2-dimethoxypropane. The ester concentrations are adequate for the determination of the positional isomers of monoethylenic fatty acids directly from the reaction mixture, using a hydrogen flame ionization gas–liquid chromatography detector. Dimethyl sulfoxide was not required to prevent the breakdown of 2,2-dimethoxypropane under the conditions employed.

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