Extracellular polysaccharide from cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1972 ◽  
Vol 50 (10) ◽  
pp. 2031-2037 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Higher Plants ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Cell Number ◽  
Suspension Cultures ◽  
Logarithmic Phase ◽  
Bush Bean ◽  
Culture Cycle ◽  
High Yields

High yields of extracellular polysaccharide were obtained from cell suspension cultures of root, hypocotyl, and cotyledon of bush bean. Hydrolysates of the three polysaccharide samples contained the same sugars: galacturonic acid, galactose, glucose, mannose, arabinose, and xylose. The relative amounts of the six sugars were not the same in the hydrolysates from the three sources. The extracellular polysaccharide was produced at all times during the culture cycle. Semilogarithmic plots of increase in cell number, and production of extracellular polysaccharide, indicate that production per cell decreased during the logarithmic phase, and increased at the onset of the stationary phase. Production of extracellular polysaccharide, per culture and per cell, was much higher than that reported for other cell cultures of higher plants.

Effect of either 2,4-dichlorophenoxyacetic acid or kinetin on production and composition of various fractions from extracellular polysaccharides produced by cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (15) ◽  
pp. 1816-1822 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Suspension Cultures ◽  
Extracellular Polysaccharides ◽  
Dichlorophenoxyacetic Acid ◽  
Neutral Sugar ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
2,4 Dichlorophenoxyacetic Acid

Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth. Kinetin was not essential but was required for optimum growth. Both of the regulators were required for optimum production of extracellular polysaccharide (EP).The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide) isolated from the EP at various stages of the culture cycle.The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments. Changes in composition of the neutral polysaccharide were slight. As a consequence of these results it is now possible to obtain cells differing in the nature of the systems synthesizing, or controlling the synthesis of, the polysaccharides.

Comparison of growth and extracellular polysaccharide of cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender) grown in coconut-milk medium and synthetic medium

1975 ◽  
Vol 53 (15) ◽  
pp. 1542-1548 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Synthetic Medium ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Suspension Cultures ◽  
Coconut Milk ◽  
Neutral Sugar ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
Neutral Sugars ◽  
Culture Cycle

Growth and extracellular polysaccharide (EP) production by cotyledon suspension cultures of bush bean were compared in coconut-milk (CM) medium and synthetic medium. EP was produced at all stages of the culture cycle investigated. The same neutral sugars were present in the EP recovered from both media and the composition showed some changes during the culture cycle. Factors in coconut milk had relatively little effect on the neutral sugar composition of the EP.

Callus and cell suspension culture of bush bean (Phaseolus vulgaris)

1970 ◽  
Vol 48 (6) ◽  
pp. 1119-1130 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Cell Number ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Coconut Milk ◽  
Mineral Salts ◽  
Bush Bean

A solid medium was developed for callus cultures originating from explants of root, hypocotyl, and cotyledon of seedlings of bush bean, and a liquid medium was developed for the growth of cell suspension cultures derived from the callus cultures. Some unsatisfactory media are recorded. Concentrations of mineral salts for cell suspension cultures are lower than for callus cultures. Both coconut milk and other organic substances are required for maximum growth. With cell suspensions the effect of deproteinized coconut milk is the same as that of raw coconut milk but, with callus cultures, deproteinized coconut milk gives greater yield. There are no obvious differences in yield of callus derived from root, hypocotyl, or cotyledon. Few differences in yield were obtained between cell suspension cultures from root, hypocotyl, and cotyledon but those from root gave the highest yield in dry weight. However, in the same medium, cells from the three origins are very similar in form and appearance. Some effects of different media on cell form and clumping are described. The yield in suspension culture is very high. Increase in cell number, fresh weight, and dry weight is about 100-fold in 12 days involving about six to seven divisions per cell.

Growth, and patterns of growth and division, in cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1971 ◽  
Vol 49 (7) ◽  
pp. 1131-1139 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Growth Regulation ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Cell Types ◽  
Growth Cycle ◽  
Cell Number ◽  
Suspension Cultures ◽  
Growth Patterns ◽  
Bush Bean

Changes in cell number, cell size, fresh weight, and dry weight were recorded for the growth cycle of a serially subcultured cell suspension culture derived from hypocotyl of bush bean (var. Contender). The various cell types and growth patterns from single cells were also recorded. The culture showed unique features including a relatively sharp separation of a phase of exponential cell division from a phase of cell expansion. Complete separation of cell clumps gave a free cell culture, of viable cells, by day 16. The growth cycle, cell types, and growth patterns are compared with those in other plant cell suspension cultures. The potential value of the culture for study of growth regulation is discussed.

A survey of the presence of glutamate synthase in plant cell suspension cultures

1976 ◽  
Vol 54 (24) ◽  
pp. 2924-2927 ◽  
Author(s):  
Donald K. Dougall ◽  
Jeff Bloch
Keyword(s):  
Cell Suspension ◽  
Higher Plants ◽  
Cell Suspension Cultures ◽  
Glutamate Synthase ◽  
Pyridine Nucleotide ◽  
Suspension Cultures ◽  
Higher Plant ◽  
Plant Cell Suspension Cultures ◽  
Plant Cell Suspension ◽  
Glutamic Dehydrogenase

Evidence was sought for the presence of glutamate synthase (EC 2.6.1.53) in extracts from suspension cultures of six higher plant species not previously examined. The level of glutamate synthase measured was above the level of glutamic dehydrogenase (EC 1.4.1.2, 1.4.1.4) in extracts of soybean, parsley, okra, and cotton. Glutamate synthase was detectable but less than glutamic dehydrogenase in extracts of sugarcane. Glutamate synthase was not detected in extracts of peanut. Evidence for two glutamate synthases, each specific for one pyridine nucleotide, was obtained with cultures of carrot. Glutamate synthase has now been detected in eight and possibly nine species representing four and possibly five families of higher plants.

Factors Affecting Protoplast Isolation and the Regeneration of Shoot-Like Structures From Protoplast-Derived Callus of Sugarcane (Saccharum spp Hybrids)

1992 ◽  
Vol 40 (6) ◽  
pp. 863 ◽  
Author(s):  
PWJ Taylor ◽  
HL Ko ◽  
SW Adkins
Keyword(s):  
Cell Suspension ◽  
Cell Walls ◽  
Cell Suspension Cultures ◽  
Protoplast Isolation ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Factors Affecting ◽  
Nodular Callus ◽  
Homogeneous Cell ◽  
High Yields

High yields of protoplasts were isolated from non-regenerable, homogeneous cell suspension cultures of sugarcane, compared with regenerable, heterogeneous cell suspension cultures after incubation in an enzyme composition containing Cellulase RS, Pectinase, Macerozyme and Driselase. Higher yields of protoplasts were released from heterogeneous cell suspension cultures after the addition of 10 mg L-1 silver nitrate to the culture medium; however, ethylene production was not involved in protoplast isolation. Use of 0-05-2% Pectolyase Y23 pectinase rather than other pectinases resulted in higher yields of protoplasts from heterogeneous cell suspension cultures. These results suggest that there are differences in the cell walls between cells from heterogeneous and homogeneous cell suspension cultures which affect the isolation of protoplasts. Protoplasts isolated from heterogeneous cell suspension cultures failed to develop beyond the cell division stage. Protoplasts isolated from homogeneous cell suspension cultures and cultured in agarose droplets bathed in modified 8p medium, reformed cell walls, divided and developed into microcallus. Microcallus transferred to solid modified MS medium containing 1 mg -1 .2,4-D developed into callus. Protoplast-derived callus from one cultivar formed compact nodular callus when subcultured onto the same medium containing 1% activated charcoal. Incubation of this callus on MS medium containing BAP at 0.5 mg L-1, then a combination of BAP and fluridone each at 0.5 mg L-1, resulted in the regeneration of small, chlorophyll-containing shoot-like structures. As yet no intact plants have developed from these shoot-like structures.

scholarly journals Cell suspension culture of Amsonia tabernaemontana Walter: growth, organogenesis and alkaloid production

2014 ◽  
Vol 50 (4) ◽  
pp. 615-624 ◽  
Author(s):  
Mirosława Furmanowa ◽  
Lucyna Rapczewska
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Cell Number ◽  
Growth Increment ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Alkaloid Production ◽  
Cellular Aggregates

The paper discusses the growth of cell suspension cultures of <em>Amsonia tabernaemontana</em> Walter established from callus of hypocotyl origin. The cell number and growth increment were determined. Cellular aggregates developed well in the Wood and Braun (WB) medium with 1 mg/l NAA and 0.5 mg/l kinetin (growth increment 712.4). When the aggregates were cultured on WB media without NAA and kinetin or with 0.02 mg/l kinetin and 3 mg/l IAA, Toots developed an the aggregates. Examiination of the roots and cell suspensions indicates that the Toots are richer in alkaloids than the callus and cell suspensions.

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