The Galium trifidum group (Galium sect. Aparinoides, Rubiaceae)
By using morphology, karyology, pollen size, leaf flavonoids, ecological observations, and modification experiments, a new classification of the Galium trifidum group is proposed. Nine taxa in five species are recognized: (1) G. tinctorium, with ssp. tinctorium and sap. floridanum (new comb.) in eastern North America; (2) G. brevipes, a rare species centered in the Great Lakes region; (3) G. trifidum, with ssp. trifidum in northern North America. Asia, and Europe, ssp. columbianum (new comb.) in (north)western North America and (north)eastern Asia, and ssp. subbiflorum (new comb.) and ssp. halophilum (new comb.) in northern North America; (4) G. innocuum in southeastern Asia; (5) G. karakulense in central Asia.New chromosome counts of n = 12 and 2n = 24 are reported for G. tinctorium ssp. tinctorium and ssp. floridanum; and G. trifidum ssp. trifidum, ssp. columbianum, and ssp. subbiflorum.