Occurrence of Aspergillus niger strains on a polychrome cotton painting and their elimination by anoxic treatment

2020 ◽  
Vol 66 (10) ◽  
pp. 586-592
Author(s):  
Douglas Boniek ◽  
Luciana Bonadio ◽  
Quésia Souza Damaceno ◽  
Antônio Fernando Batista dos Santos ◽  
Maria Aparecida de Resende Stoianoff
Keyword(s):  
Aspergillus Niger ◽  
Conservation Status ◽  
Microbial Metabolism ◽  
Fungal Species ◽  
Microbial Populations ◽  
Nondestructive Technique ◽  
Microbiological Techniques ◽  
Black Fungi ◽  
Metabolic Action ◽  
Total Elimination

This study aimed to isolate and identify the population of filamentous fungi colonizing a cotton painting, whose conservation status was compromised and showed signs of biodeterioration due to dirt accumulation and microbial metabolism. In addition, microbiological techniques such as cultivation-dependent approach and molecular biology were used to identify microbial populations and to eliminate their metabolic action. For this, the nondestructive anoxic atmosphere technique was used, in which the microbial metabolism was affected by the absence of oxygen. Prior to exposure to an anoxic atmosphere, only one fungal species, Aspergillus niger, was identified at 12 points sampled in the obverse and reverse of the artwork; no fungal species persisted as a result of anoxic treatment. These results showed that exposure to anoxic conditions was effective for the total elimination of isolated fungal strains as well as their spores. In conclusion, this study proved the unprecedented effectiveness of a nondestructive technique for artwork on textile colonized by black fungi species. Thus, this interdisciplinary work involving conservation, microbiology, and chemistry presents a tool to eliminate microorganisms, while maintaining the integrity of artwork and safety of the restorer, that can be applied prior to artwork restoration.

Phosphate Solubilization Potentials of Rhizosphere Fungi Isolated from Insecticide Treated Soil

2020 ◽  
Vol 4 (1) ◽  
pp. 58-69
Author(s):  
Babatide Olufemi Oladapo ◽  
Esther Aanuoluwa Ekundayo ◽  
Mariam Olukemi Mokoolu ◽  
Fatuyi Olanipekun Ekundayo
Keyword(s):  
Incubation Time ◽  
Phosphate Solubilization ◽  
Trichoderma Viride ◽  
Fungal Species ◽  
Rhizosphere Fungi ◽  
Optimization Study ◽  
Lambda Cyhalothrin ◽  
Optimum Ph ◽  
Microbiological Techniques ◽  
Tricalcium Phosphates

Abstract Insecticides are used widely to control a variety of pests and often residues of these insecticides are left in soil which may have impact on the phosphate solubilization potentials of rhizosphere fungi. Rhizosphere soils were collected from carefully uprooted cowpea seedlings with hand trowel on 50, 70 and 90th days of germination containing the lambda-cyhalothrin and dimethoate insecticides. Fungi associated with the above samples were identified by standard microbiological techniques. Screening for phosphate solubilization potential of the isolates was done by spot inoculation on Pikovskaya agar by measuring the clear zones around the colonies supplemented with tricalcium phosphates [Ca3(PO4)2]. The phosphatase produced by the fungal species was optimized using parameters such as incubation time, pH, temperature, carbon source and nitrogen source in submerged fermentation. The isolated rhizosphere fungi were identified as Rhizopus stolonifer, Aspergillus niger, Aspergillus fumigatus, Aspergillus terreus, Trichoderma viride, Arthroderma fulvum and Fusarium oxysporum. Among the isolates, Trichoderma viride showed the best ability to solubilize phosphate with solubilization index of 2.82 with dimethoate of 12.5ml/L of water. The optimization study for the enzyme production showed that the best incubation time for phosphatase production was 72 hours by Trichoderma viride. It was observed that the optimum pH for production of phosphatase by Trichoderma viride was 6.5. This study suggests that all the isolated fungi especially Trichoderma viride can adapt to these insecticidal treatments, which make them useful as biofertilizers to increase uptake of phosphorous in plants.

scholarly journals Fungal Population and Physicochemical Characteristics of Abattoir-Impacted Soil in Iwofe, Rivers State

2021 ◽  
pp. 36-44
Author(s):  
Williams, Janet Olufunmilayo ◽  
Owhorji, Gloria
Keyword(s):  
Heavy Metals ◽  
Aspergillus Niger ◽  
Physicochemical Characteristics ◽  
Control Soil ◽  
Fungal Population ◽  
Candida Sp ◽  
Fungal Isolates ◽  
Fungal Load ◽  
Microbiological Techniques ◽  
Rivers State

Aim: To determine the fungal population and physicochemistry of abattoir impacted soil in Iwofe, Rivers State. Study Design: This study focused on Abattoir impacted soil. Statistical analysis of data and interpretation was carried out. Place and Duration of Study: Abattoir impacted soil was collected from three points in an abattoir located in Iwofe, Rivers State while the unpolluted soil which served as control was collected from the Rivers State University, Port Harcourt in January, 2021. Methodology: Standard microbiological techniques were used: the fungal population was determined by inoculating aliquots of an appropriate dilution resulting from a ten-fold serial dilution on prepared Sabouraud dextrose agar plates in duplicates. Plates were later incubated for 3-5 days after which colonies were enumerated and used in obtaining the fungal population in the soil samples while distinct colonies were subcultured for macroscopic and microscopic identification of fungi. The physicochemical parameters and heavy metals were analyzed using standard methods. Results: Fungal load in the control and abattoir impacted soil were 1.09×105 and 3.9×104 CFU/g, respectively. The fungal load of the control soil was significantly higher (P˂0.05) than the abattoir impacted soil. The fungal isolates identified in the abattoir impacted soil were Microsporium sp, Aspergillus niger and Candida sp while Aspergillus niger, Aspergillus flavus, Fusarium sp, Penicillium sp, Mucor sp and Rhizopus sp were identified from the control soil. The pH, temperature, nitrate and phosphate of the abattoir soil were 6.7, 28.33℃, 27.83(mgKg-1) and 1055(mgKg-1), respectively. The concentrations of Cadmium, Iron and Lead in the abattoir Impacted soil and control soil were 0.81, 563.35 and 7.12 mgKg-1, 0.51, 582.0 and 3.18 mgKg-1, respectively. The physico chemistry and heavy metals in the abattoir soil were within acceptable limits. Discussion and Conclusion: The findings from this study showed that heavy metals in abattoir impacted soil had an impact in the fungal population which led to the isolation of only three fungal isolates belonging to Microsporium sp, Candida sp and Aspergillus niger. More so, despite the presence of heavy metals in the abattoir impacted soil, the metals were all within permissible limits. Thus, the abattoir impacted soil was not heavily polluted.

scholarly journals Airborne Aspergillus at some rural areas adjoining to Raipur city (C.G.) India

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Ritu Kunjam ◽  
V.K. Kanungo ◽  
S.K. Jadhav
Keyword(s):  
Aspergillus Niger ◽  
Rural Areas ◽  
Meteorological Parameters ◽  
Agar Medium ◽  
Fungal Species ◽  
Aspergillus Clavatus ◽  
Potato Dextrose Agar Medium ◽  
Percentage Contribution ◽  
Different Seasons

Increased urbanization and industrialization in recent time has made a significant impact on air quality of the area. The atmosphere is rich in propagule of different fungal species. The investigation on airborne Aspergillus contribution was conducted in Periphery of Raipur city from February, 2018 to March, 2019 with the help of gravity petriplate containing PDA (Potato Dextrose Agar) medium. In this study, total 11 species of Aspergillus were recorded. The percentage frequency and percentage contribution of different Aspergillus species were different in different seasons. Aspergillus niger was most frequent throughout the year followed by Aspergillus fumigatus, A. flavus, and A. nidulans etc. While Aspergillus clavatus, and A. versicolor, A. aculeatus were the least frequent species. The result indicated the highest percentage contribution of Aspergillus niger (43.29 percent) followed by A. fumigatus (9.02percent), A. flavus (8.42 percent) while A. clavatus (0.21 percent). The objective of the studies was to determine a seasonal variation in concentrations of Aspergillus on the basis of meteorological parameters.

scholarly journals Molds in museum environments: Biodeterioration of art photographs and wooden sculptures

2013 ◽  
Vol 65 (3) ◽  
pp. 955-962 ◽  
Author(s):  
Milica Ljaljevic-Grbic ◽  
M. Stupar ◽  
Jelena Vukojevic ◽  
Ivana Maricic ◽  
Natasa Bungur
Keyword(s):  
Aspergillus Niger ◽  
Indoor Air ◽  
Trichoderma Viride ◽  
Fungal Species ◽  
Chaetomium Globosum ◽  
Fungal Colonization ◽  
Cellulolytic Activity ◽  
Cultural Center ◽  
Fungal Propagules ◽  
Alternaria Sp

Pieces of art stored in museum depots and display rooms are subject to fungal colonization that leads to bio-deterioration processes. Deteriorated wooden sculptures and art photographs temporarily stored in the quarantine room of the Cultural Center of Belgrade were subject to mycological analyses. Twelve fungal species were identified on the wooden substratum and five species were detected on photograph surfaces. Trichoderma viride, Chaetomium globosum and Alternaria sp. were the fungi with proven cellulolytic activity detected on the examined cellulose substrata. Indoor air mycobiota were estimated to 210.09 ? 8.06 CFU m-3, and the conidia of fungus Aspergillus niger were the dominant fungal propagules in the air of the examined room.

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

scholarly journals Antifungal Activity of Plant Extracts against Aspergillus niger and Rhizopus stolonifer

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Venkatasaichaitanya Surapuram ◽  
William N. Setzer ◽  
Robert L. McFeeters ◽  
Hana McFeeters
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Human Health ◽  
Plant Extracts ◽  
Food Supply ◽  
Fungal Species ◽  
Rhizopus Stolonifer ◽  
Antifungal Properties ◽  
Recent Advances

Despite recent advances in antifungal development, fungi remain a devastating threat to human health and compromise viability of the food supply. Plant based antimicrobials represent a vast untapped source with tremendous potential. Herein we present the antifungal properties of more than 50 plant extracts against two important human and agricultural pathogens, Aspergillus niger and Rhizopus stolonifer. Multiple extracts exhibit promising MIC values of less than 100 μg/mL and are reported for both fungal species.

Microbial metabolism of methyl protodioscin by Aspergillus niger culture—A new androstenedione producing way from steroid

2006 ◽  
Vol 100 (1-3) ◽  
pp. 87-94 ◽  
Author(s):  
Xiangjiu He ◽  
Bo Liu ◽  
Guanghui Wang ◽  
Xinluan Wang ◽  
Lina Su ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Microbial Metabolism

scholarly journals Aspergillus niger causing tracheobronchitis and invasive pulmonary aspergillosis in a lung transplant recipient: case report

2008 ◽  
Vol 41 (2) ◽  
pp. 200-201 ◽  
Author(s):  
Melissa Orzechowski Xavier ◽  
Maria da Penha Uchoa Sales ◽  
José de Jesus Peixoto Camargo ◽  
Alessandro Comarú Pasqualotto ◽  
Luiz Carlos Severo
Keyword(s):  
Case Report ◽  
Aspergillus Niger ◽  
Transplant Recipient ◽  
Cytomegalovirus Infection ◽  
Lung Transplant ◽  
Invasive Pulmonary Aspergillosis ◽  
Invasive Disease ◽  
Fungal Species ◽  
Lung Transplant Recipient ◽  
Pulmonary Aspergillosis

A case of invasive aspergillosis caused by Aspergillus niger in a lung transplant recipient is described. The patient presented hyperglycemia starting postoperatively, with other complications such as cytomegalovirus infection. The associated predisposing factors and other implications are discussed. Aspergillus niger seems to be a fungal species of low virulence that requires the presence of a severely immunosuppressed host to cause invasive disease.

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