Molecular cytogenetic characterization and stripe rust response of a trigeneric hybrid involving Triticum, Psathyrostachys, and Thinopyrum

Genome ◽  
2012 ◽  
Vol 55 (5) ◽  
pp. 383-390 ◽  
Author(s):  
Houyang Kang ◽  
Jian Zeng ◽  
Quan Xie ◽  
Shan Tao ◽  
Meiyu Zhong ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Triticum Aestivum L ◽  
Thinopyrum Intermedium ◽  
Psathyrostachys Huashanica ◽  
Molecular Cytogenetic ◽  
Meiotic Configuration ◽  
Cytogenetic Characterization ◽  
The Mean ◽  
Trigeneric Hybrids ◽  
Gish Analysis

Trigeneric hybrids offer opportunities to transfer alien traits into cultivated wheat. In this study, a new trigeneric hybrid involving species of Triticum , Psathyrostachys , and Thinopyrum was synthesized by crossing Triticum aestivum L. (wheat) – Thinopyrum intermedium (Host) Barkworth & D.R. Dewey amphiploid Zhong 3 with wheat – Psathyrostachys huashanica Keng ex Kuo amphiploid PHW-SA. Crossability of the two amphiploids was 19.74%, and the fertility of the hybrid was 16.20%. The mean meiotic configuration of the trigeneric hybrid (2n = 56) was 13.06 I + 17.24 IIring + 3.73 IIrod + 0.28 III + 0.04 IV. GISH analysis indicated that the trigeneric F1 had seven P. huashanica chromosomes and seven Th. intermedium chromosomes. The mean chromosome numbers of F2, F3, and F4 progenies were 2n = 49.24, 2n = 48.13, and 2n = 46.78, respectively, a gradual decrease. GISH analysis revealed that most F2 and F3 plants had 2–10 Th. intermedium chromosomes and 0–4 P. huashanica chromosomes. In the F4 progenies, 1–7 Th. intermedium chromosomes were labeled, but no P. huashanica chromosomes were detected. It seems that Th. intermedium chromosomes are more likely than P. huashanica chromosomes to be transmitted to the progenies. The stripe rust response of PHW-SA was expressed in the F1 and some F2 and F3 progenies. The trigeneric hybrid could be a useful bridge for transfering P. huashanica and Th. intermedium chromosomes to common wheat.

scholarly journals Cytogenetic study and stripe rust response of the derivatives from a wheat – Thinopyrum intermedium – Psathyrostachys huashanica trigeneric hybrid

Genome ◽  
2017 ◽  
Vol 60 (5) ◽  
pp. 393-401 ◽  
Author(s):  
Hou-Yang Kang ◽  
Lin Tang ◽  
Dai-Yan Li ◽  
Cheng-Dou Diao ◽  
Wei Zhu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Cytogenetic Study ◽  
Stripe Rust Resistance ◽  
Recurrent Parent ◽  
Meiotic Behavior ◽  
Thinopyrum Intermedium ◽  
Psathyrostachys Huashanica ◽  
Meiotic Configuration ◽  
Partial Amphiploid

To transfer multiple desirable alien genes into common wheat, we previously reported a new trigeneric hybrid synthesized by crossing a wheat – Thinopyrum intermedium partial amphiploid with wheat – Psathyrostachys huashanica amphiploid. Here, the meiotic behavior, chromosome constitution, and stripe rust resistance of F5 derivatives from the wheat – Th. intermedium – P. huashanica trigeneric hybrid were studied. Cytological analysis indicated the F5 progenies had chromosome numbers of 42–50 (average 44.96). The mean meiotic configuration was 1.28 univalents, 21.74 bivalents, 0.04 trivalents, and 0.02 tetravalents per pollen mother cell. In 2n = 42 lines, the average pairing configuration was 0.05 I + 19.91 II (ring) + 1.06 II (rod) + 0.003 IV, suggesting these lines were cytologically stable. Most lines with 2n = 43, 44, 46, 48, or 50, bearing a high frequency of univalents or multivalents, showed abnormal meiotic behavior. Genomic in situ hybridization karyotyping results revealed that 25 lines contained 1–7 Th. intermedium chromosomes, but no P. huashanica chromosomes were found among the 27 self-pollinated progenies. At meiosis, univalents (1–5) possessing Th. intermedium hybridization signals were detected in 19 lines. Bivalents (1–3) expressing fluorescence signals were observed in 12 lines. Importantly, 21 lines harbored wheat – Th. intermedium chromosomal translocations with various alien translocation types. Additionally, two homozygous lines, K13-668-10 and K13-682-12, possessed a pair of wheat – Th. intermedium small fragmental translocations. Compared with the recurrent parent Zhong 3, most lines showed high resistance to the stripe rust (Puccinia striiformis f. sp. tritici) pathogens prevalent in China, including race V26/Gui22. This paper reports a highly efficient technical method for inducing alien translocation between wheat and Th. intermedium by trigeneric hybridization. These lines might be potentially valuable germplasm resources for further wheat improvement.

Molecular cytogenetic characterization of two high protein wheat-Thinopyrum intermedium partial amphiploids

2011 ◽  
Vol 52 (3) ◽  
pp. 269-277 ◽  
Author(s):  
Mariyana Georgieva ◽  
Adél Sepsi ◽  
Nedyalka Tyankova ◽  
Márta Molnár-Láng
Keyword(s):  
High Protein ◽  
Thinopyrum Intermedium ◽  
Molecular Cytogenetic ◽  
Cytogenetic Characterization ◽  
Partial Amphiploids

Molecular cytogenetic characterization of a new wheat Secale africanum 2Ra(2D) substitution line for resistance to stripe rust

2011 ◽  
Vol 90 (2) ◽  
pp. 283-287 ◽  
Author(s):  
MENGPING LEI ◽  
GUANGRONG LI ◽  
SUFEN ZHANG ◽  
CHENG LIU ◽  
ZUJUN YANG
Keyword(s):  
Stripe Rust ◽  
Substitution Line ◽  
Molecular Cytogenetic ◽  
Secale Africanum ◽  
Cytogenetic Characterization

Molecular cytogenetic characterization of wheat–Secale africanum amphiploids and derived introgression lines with stripe rust resistance

Euphytica ◽  
2008 ◽  
Vol 167 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Zu-Jun Yang ◽  
Guang-Rong Li ◽  
Ju-Qing Jia ◽  
Xue Zeng ◽  
Meng-Ping Lei ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Introgression Lines ◽  
Stripe Rust Resistance ◽  
Molecular Cytogenetic ◽  
Secale Africanum ◽  
Cytogenetic Characterization
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