A comparative study of the structure of the rDNA intergenic spacer of Lens culinaris Medik., and other legume species

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 597-603 ◽  
Author(s):  
M. Fernández ◽  
C. Polanco ◽  
M.L. Ruiz ◽  
M. Pérez de la Vega
Keyword(s):  
Comparative Study ◽  
Lens Culinaris ◽  
Intergenic Spacer ◽  
Legume Species ◽  
Rdna Intergenic Spacer

A comparative study of the structure of the rDNA intergenic spacer ofLens culinarisMedik., and other legume species

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 597-603 ◽  
Author(s):  
M Fernández ◽  
C Polanco ◽  
M L Ruiz ◽  
M Pérez de la Vega
Keyword(s):  
Transcription Initiation ◽  
Lens Culinaris ◽  
Pcr Amplification ◽  
Intergenic Spacer ◽  
Initiation Site ◽  
Legume Species ◽  
Consensus Sequences ◽  
Polymerase Chain ◽  
Rdna Intergenic Spacer ◽  
Enhancer Sequences

As part of a project on lentil molecular genetics, the sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS) of Lens culinaris Medik. was determined. DNA was cloned after polymerase chain reaction (PCR) amplification. The spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of repeated sequences, named A to D. C and D arrays were formed by the repetition of very short consensus sequences. Similarity was found between lentil and other legume species, in particular those of the Vicieae tribe. A transcription initiation site, putative sites of termination and processing, and promoter-enhancer sequences were detected by computer-aided searches. These sites resemble motifs conserved in the IGS sequences of other plant species. The conservation of motifs in the otherwise highly variable plant IGS sequences points to the relevance of these motifs as functional sequences.Key words: legumes, Lens culinaris Medik., lentil, rDNA intergenic spacer, IGS, structure.

scholarly journals Polymorphism of 5S rDNA intergenic spacer in some Gentiana species

1970 ◽  
pp. 42-46
Author(s):  
I. O. Andreev ◽  
V. M. Melnyk ◽  
G. Yu. Myryuta ◽  
V. A. Kunakh
Keyword(s):  
Intergenic Spacer ◽  
5S Rdna ◽  
Rdna Intergenic Spacer

Fine structure and evolution of the rDNA intergenic spacer in rice and other cereals

1993 ◽  
Vol 36 (4) ◽  
Author(s):  
F. Cordesse ◽  
R. Cooke ◽  
D. Tremousaygue ◽  
F. Grellet ◽  
M. Delseny
Keyword(s):  
Fine Structure ◽  
Intergenic Spacer ◽  
Rdna Intergenic Spacer

25S-18S rDNA intergenic spacer ofNicotiana sylvestris (Solanaceae): Primary and secondary structure analysis

1999 ◽  
Vol 218 (1-2) ◽  
pp. 89-97 ◽  
Author(s):  
R. A. Volkov ◽  
A. Bachmair ◽  
I. I. Panchuk ◽  
S. S. Kostyshyn ◽  
D. Schweizer
Keyword(s):  
Secondary Structure ◽  
Structure Analysis ◽  
18S Rdna ◽  
Intergenic Spacer ◽  
Secondary Structure Analysis ◽  
Rdna Intergenic Spacer

Cultivar identification using 18S–28S rDNA intergenic spacer-RFLP in pomegranate (Punica granatum L.)

2009 ◽  
Vol 120 (4) ◽  
pp. 500-503 ◽  
Author(s):  
P. Melgarejo ◽  
J.J. Martínez ◽  
Fca. Hernández ◽  
R. Martínez ◽  
P. Legua ◽  
...  
Keyword(s):  
Cultivar Identification ◽  
Intergenic Spacer ◽  
Punica Granatum ◽  
28S Rdna ◽  
Rdna Intergenic Spacer ◽  
Punica Granatum L
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