scholarly journals Chaperone-driven polymer translocation through nanopore: Spatial distribution and binding energy

2017 ◽  
Vol 40 (4) ◽  
Author(s):  
Rouhollah Haji Abdolvahab
1988 ◽  
Vol 3 (3) ◽  
pp. 506-513 ◽  
Author(s):  
J. Halbritter

Angle-resolved x-ray photoelectron spectroscopy (ARXPS) results in a very detailed analysis of minor amounts (≥0.3 nm) of interface compounds and their spatial distribution. First experimental results on the Si–SiO2 interface used in microelectronics, are presented. The ARXPS results on plane, single crystalline (100) Si, oxidized to about 5 nm SiO2, indicate a planar Si surface connected by about one monolayer Si∗ to a compressed SiO∗2 layer coated by SiO2. Separated from this interface region, Si clusters stabilized by a compressing SiO∗2 coating have been found in SiO2. Dehydrogenation is showing up in binding energy changes in SiO2 indicating that H or OH is not only saturating Si∗ but is also bonded to SiO2. The O deficiency of amorphous SiO2,x is increasing toward the outer SiO2 surface.


2014 ◽  
Vol 10 (S312) ◽  
pp. 237-238
Author(s):  
Jongsuk Hong ◽  
Enrico Vesperini ◽  
Antonio Sollima ◽  
Steve McMillan ◽  
Franca D'Antona ◽  
...  

AbstractWe have performed a survey of N-body simulations to explore the dynamics of primordial binaries in multiple-population globular clusters. We show that, as a consequence of the initial differences between the spatial distribution of first-generation (FG) and second-generation (SG) stars, SG binaries are disrupted more efficiently than FG binaries. The effects of dynamical evolution on the surviving binaries produces a difference between the SG and the FG binary binding energy distribution with the SG population characterized by a larger fraction of high binding energy (more bound) binaries. We also explore the evolution of the radial variation of the SG-to-FG binary number ratio and find that although the global binary fraction decreases more rapidly for the SG population, the local binary fraction measured in the cluster inner regions may still be dominated by SG binaries.


Author(s):  
L. D. Jackel

Most production electron beam lithography systems can pattern minimum features a few tenths of a micron across. Linewidth in these systems is usually limited by the quality of the exposing beam and by electron scattering in the resist and substrate. By using a smaller spot along with exposure techniques that minimize scattering and its effects, laboratory e-beam lithography systems can now make features hundredths of a micron wide on standard substrate material. This talk will outline sane of these high- resolution e-beam lithography techniques.We first consider parameters of the exposure process that limit resolution in organic resists. For concreteness suppose that we have a “positive” resist in which exposing electrons break bonds in the resist molecules thus increasing the exposed resist's solubility in a developer. Ihe attainable resolution is obviously limited by the overall width of the exposing beam, but the spatial distribution of the beam intensity, the beam “profile” , also contributes to the resolution. Depending on the local electron dose, more or less resist bonds are broken resulting in slower or faster dissolution in the developer.


Author(s):  
Jayesh Bellare

Seeing is believing, but only after the sample preparation technique has received a systematic study and a full record is made of the treatment the sample gets.For microstructured liquids and suspensions, fast-freeze thermal fixation and cold-stage microscopy is perhaps the least artifact-laden technique. In the double-film specimen preparation technique, a layer of liquid sample is trapped between 100- and 400-mesh polymer (polyimide, PI) coated grids. Blotting against filter paper drains excess liquid and provides a thin specimen, which is fast-frozen by plunging into liquid nitrogen. This frozen sandwich (Fig. 1) is mounted in a cooling holder and viewed in TEM.Though extremely promising for visualization of liquid microstructures, this double-film technique suffers from a) ireproducibility and nonuniformity of sample thickness, b) low yield of imageable grid squares and c) nonuniform spatial distribution of particulates, which results in fewer being imaged.


Author(s):  
Auclair Gilles ◽  
Benoit Danièle

During these last 10 years, high performance correction procedures have been developed for classical EPMA, and it is nowadays possible to obtain accurate quantitative analysis even for soft X-ray radiations. It is also possible to perform EPMA by adapting this accurate quantitative procedures to unusual applications such as the measurement of the segregation on wide areas in as-cast and sheet steel products.The main objection for analysis of segregation in steel by means of a line-scan mode is that it requires a very heavy sampling plan to make sure that the most significant points are analyzed. Moreover only local chemical information is obtained whereas mechanical properties are also dependant on the volume fraction and the spatial distribution of highly segregated zones. For these reasons we have chosen to systematically acquire X-ray calibrated mappings which give pictures similar to optical micrographs. Although mapping requires lengthy acquisition time there is a corresponding increase in the information given by image anlysis.


Author(s):  
Gary Bassell ◽  
Robert H. Singer

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.


Author(s):  
J.M. Robinson ◽  
J.M Oliver

Specialized regions of plasma membranes displaying lateral heterogeneity are the focus of this Symposium. Specialized membrane domains are known for certain cell types such as differentiated epithelial cells where lateral heterogeneity in lipids and proteins exists between the apical and basolateral portions of the plasma membrane. Lateral heterogeneity and the presence of microdomains in membranes that are uniform in appearance have been more difficult to establish. Nonetheless a number of studies have provided evidence for membrane microdomains and indicated a functional importance for these structures.This symposium will focus on the use of various imaging modalities and related approaches to define membrane microdomains in a number of cell types. The importance of existing as well as emerging imaging technologies for use in the elucidation of membrane microdomains will be highlighted. The organization of membrane microdomains in terms of dimensions and spatial distribution is of considerable interest and will be addressed in this Symposium.


Author(s):  
Clifford S. Rainey

The spatial distribution of V and Ni deposited within fluidized catalytic cracking (FCC) catalyst is studied because these metals contribute to catalyst deactivation. Y zeolite in FCC microspheres are high SiO2 aluminosilicates with molecular-sized channels that contain a mixture of lanthanoids. They must withstand high regeneration temperatures and retain acid sites needed for cracking of hydrocarbons, a process essential for efficient gasoline production. Zeolite in combination with V to form vanadates, or less diffusion in the channels due to coke formation, may deactivate catalyst. Other factors such as metal "skins", microsphere sintering, and attrition may also be involved. SEM of FCC fracture surfaces, AEM of Y zeolite, and electron microscopy of this work are developed to better understand and minimize catalyst deactivation.


Sign in / Sign up

Export Citation Format

Share Document