Microfluidic Cantilever Dynamics and Thermomechanics of DNA Melting Transitions

2021 ◽  
Vol MA2021-02 (56) ◽  
pp. 1667-1667
Author(s):  
Thomas Thundat
Keyword(s):  
Dna Melting ◽  
Melting Transitions

scholarly journals Quantifying DNA melting transitions using single-molecule force spectroscopy

2008 ◽  
Vol 21 (3) ◽  
pp. 034114 ◽  
Author(s):  
Christopher P Calderon ◽  
Wei-Hung Chen ◽  
Kuan-Jiuh Lin ◽  
Nolan C Harris ◽  
Ching-Hwa Kiang
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Dna Melting ◽  
Single Molecule Force Spectroscopy ◽  
Melting Transitions

scholarly journals Protein Quantification Using Controlled DNA Melting Transitions in Bivalent Probe Assemblies

2015 ◽  
Vol 87 (19) ◽  
pp. 9576-9579 ◽  
Author(s):  
Joonyul Kim ◽  
Juan Hu ◽  
Andresa B. Bezerra ◽  
Mark D. Holtan ◽  
Jessica C. Brooks ◽  
...  
Keyword(s):  
Protein Quantification ◽  
Dna Melting ◽  
Melting Transitions

scholarly journals High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in Solution

2005 ◽  
Vol 51 (10) ◽  
pp. 1770-1777 ◽  
Author(s):  
Luming Zhou ◽  
Lesi Wang ◽  
Robert Palais ◽  
Robert Pryor ◽  
Carl T Wittwer
Keyword(s):  
High Resolution ◽  
Factor V Leiden ◽  
Dna Melting ◽  
Sequence Variants ◽  
Specific Sequence ◽  
Mutation Scanning ◽  
Melting Curves ◽  
Pcr Product ◽  
Melting Analysis ◽  
Melting Transitions

Abstract Background: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described. Methods: Asymmetric PCR was performed in the presence of unlabeled oligonucleotide probes and a saturating fluorescent DNA dye. High-resolution melting curves for samples in either capillaries (0.3 °C/s) or microtiter format (0.1 °C/s) were generated in the same containers used for amplification. Melting curves of the factor V Leiden single-nucleotide polymorphism (SNP) and several mutations in exons 10 and 11 of the cystic fibrosis transconductance regulator gene were analyzed for both PCR product and probe melting transitions. Results: Independent verification of genotype for simple SNPs was achieved by either PCR product or probe melting transitions. Two unlabeled probes in one reaction could genotype many sequence variants with simultaneous scanning of the entire PCR product. For example, analysis of both product and probe melting transitions genotyped ΔF508, ΔI507, Q493X, I506V, and F508C variants in exon 10 and G551D, G542X, and R553X variants in exon 11. Unbiased hierarchal clustering of the melting transitions identified the specific sequence variants. Conclusions: When DNA melting is performed rapidly and observed at high resolution with saturating DNA dyes, it is possible to scan for mutations and genotype at the same time within a few minutes after amplification. The method is no more complex than PCR and may reduce the need for resequencing.

scholarly journals Base-specific pre-melting and melting transitions of DNA in presence of ionic liquids probed by synchrotron-based UV resonance Raman scattering

2021 ◽  
Vol 330 ◽  
pp. 115433
Author(s):  
Cettina Bottari ◽  
Sara Catalini ◽  
Paolo Foggi ◽  
Ines Mancini ◽  
Andrea Mele ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Raman Scattering ◽  
Resonance Raman ◽  
Resonance Raman Scattering ◽  
Uv Resonance Raman ◽  
Melting Transitions

High resolution DNA melting analysis: an application for prenatal control of α-thalassemia

2010 ◽  
pp. n/a-n/a
Author(s):  
Supatra Sirichotiyakul ◽  
Chanane Wanapirak ◽  
Rattika Saetung ◽  
Torpong Sanguansermsri
Keyword(s):  
High Resolution ◽  
Dna Melting ◽  
Melting Analysis

Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis

2007 ◽  
Vol 2 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Jesse Montgomery ◽  
Carl T Wittwer ◽  
Robert Palais ◽  
Luming Zhou
Keyword(s):  
High Resolution ◽  
Dna Melting ◽  
Mutation Scanning ◽  
Melting Analysis

scholarly journals Statistical mechanics of base stacking and pairing in DNA melting

2004 ◽  
Vol 70 (5) ◽  
Author(s):  
Vassili Ivanov ◽  
Yan Zeng ◽  
Giovanni Zocchi
Keyword(s):  
Statistical Mechanics ◽  
Dna Melting ◽  
Base Stacking

The Order of Phase Transition of Solvable DNA Melting Models

2003 ◽  
Vol 17 (16) ◽  
pp. 885-896 ◽  
Author(s):  
Su-Long Nyeo ◽  
I-Ching Yang
Keyword(s):  
Phase Transition ◽  
Hydrogen Bonds ◽  
Short Range ◽  
Scaling Laws ◽  
Dna Melting ◽  
Thermodynamic Quantities ◽  
Base Pairs ◽  
Order Of Phase Transition ◽  
Exactly Solvable ◽  
Dna Models

The phase transition of DNA molecules is studied in an exactly solvable formalism with the Morse and Deng–Fan potentials for the interstrand hydrogen bonds of nucleotide base pairs. It is shown that although the two potentials have different short-range behaviors, the thermodynamic quantities of the DNA system in these potentials enjoy the same scaling laws with the associated critical exponents, which are explicitly calculated. These exactly solvable DNA models are shown to exhibit a phase transition of the second order and the results of the analysis agree with previous studies.

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