Nucleic acid content of turkey heart with a note on hemodynamic findings

1964 ◽  
Vol 207 (5) ◽  
pp. 1102-1106
Author(s):  
Robert G. Sumner ◽  
Thomas C. Hoyle ◽  
Henry D. McIntosh ◽  
Robert E. Whalen
Keyword(s):  
Protein Concentration ◽  
Systemic Blood Pressure ◽  
Laboratory Animals ◽  
Nucleic Acid Content ◽  
Dna Concentration ◽  
Cellular Change ◽  
Marked Disparity ◽  
Left And Right ◽  
Rna Concentration

In order to study the marked disparity in thickness of the left and right ventricular (LV and RV) walls of the turkey heart, DNA, RNA, and protein content of LV and RV myocardium were determined in fowl aged 4, 6, and 11 weeks and at maturity. DNA concentration in RV tissue was significantly greater than that in LV tissue in each bird and at each age. DNA concentration in both ventricles, moreover, decreased with increasing age. Protein concentration in both ventricles increased as did protein-to-DNA ratios. Although RNA concentration showed less consistent changes with age, the RNA-to-DNA ratio increased. It was concluded that the predominant cellular change with age was hypertrophy. In addition, electrocardiograms and hemodynamic data were obtained on birds in each group. Although pulmonary artery pressure is comparable to that in other laboratory animals, a markedly elevated systemic blood pressure develops between the ages of 6 and 11 weeks. The role of hypertension as a determinant of cellular size in the LV was not elucidated by this study.

Calculations used for assessment of rapid maxillary expansion

ORL ro ◽  
2016 ◽  
Vol 1 (1) ◽  
pp. 24-26
Author(s):  
Ruxandra Bartok ◽  
Bogdan Dimitriu Dimitriu ◽  
Constantin Vârlan ◽  
Radu Stanciu ◽  
Georgiana Moldoveanu ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Elasticity Modulus ◽  
Rapid Maxillary Expansion ◽  
Laboratory Animals ◽  
Maxillary Expansion ◽  
Literature Reference ◽  
Bone Changes ◽  
Upper Jaw ◽  
Materials Used

Rapid maxillary expansion is defined as the release of medio-palatine suture using an orthopedic forces. The role of this procedure is to expand  the upper jaw in order to achieve the  broadening of the upper arch. This study was initiated to quantify the effects of disjunction and post- treatment bone changes, after an adequate contention which lasted for three weeks. This study  is  carried on laboratory animals (common breed rabbit) to determine tensile strength and the elasticity modulus of  biological materials  used in orthdodontics. The results of the study are consistent with those reported in the literature reference.  

Serum Concentration and Skin Expression of S100A7 (Psoriasin) in Patients Suffering from Hidradenitis Suppurativa

Dermatology ◽  
2020 ◽  
pp. 1-7
Author(s):  
Aleksandra Batycka-Baran ◽  
Wojciech Baran ◽  
Danuta Nowicka-Suszko ◽  
Maria Koziol-Gałczyńska ◽  
Andrzej Bieniek ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Hidradenitis Suppurativa ◽  
Protein Concentration ◽  
Normal Skin ◽  
Innate Immune ◽  
Antimicrobial Protein ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Reactive Protein

<b><i>Background:</i></b> Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease. An important role of innate immune dysregulation in the pathogenesis of HS has been highlighted. S100A7 (psoriasin) is an innate, antimicrobial protein that exerts proinflammatory and chemotactic action. <b><i>Objectives:</i></b> The objective of the study was to investigate serum concentrations of S100A7 in individuals with HS as compared to healthy controls. Further, we evaluated the expression of S100A7 in lesional HS skin as compared to perilesional (clinically uninvolved) HS skin and normal skin. <b><i>Methods:</i></b> Serum concentrations of S100A7 were evaluated with a commercially available ELISA kit. The expression of S100A7 in the skin was assessed using qRT-PCR and immunofluorescence staining. <b><i>Results:</i></b> We found increased expression of S100A7 in lesional HS skin as compared to perilesional HS skin (<i>p</i> = 0.0017). The expression of S100A7 in lesional HS skin was positively associated with serum C-reactive protein concentration and the severity of disease according to Hurley staging. The serum concentration of S100A7 in individuals with HS was decreased as compared to healthy controls and patients with psoriasis. <b><i>Conclusions:</i></b> Upregulated in lesional HS skin, S100A7 may enhance the inflammatory process and contribute to the HS pathogenesis.

scholarly journals Effect of corpus callosum agenesis on the language network in children and adolescents

2021 ◽  
Author(s):  
Lisa Bartha-Doering ◽  
Ernst Schwartz ◽  
Kathrin Kollndorfer ◽  
Florian Ph. S. Fischmeister ◽  
Astrid Novak ◽  
...  
Keyword(s):  
Functional Connectivity ◽  
Corpus Callosum ◽  
Network Connectivity ◽  
Functional Language ◽  
Healthy Controls ◽  
Language Abilities ◽  
Verbal Abilities ◽  
Left And Right ◽  
Language Network

AbstractThe present study is interested in the role of the corpus callosum in the development of the language network. We, therefore, investigated language abilities and the language network using task-based fMRI in three cases of complete agenesis of the corpus callosum (ACC), three cases of partial ACC and six controls. Although the children with complete ACC revealed impaired functions in specific language domains, no child with partial ACC showed a test score below average. As a group, ACC children performed significantly worse than healthy controls in verbal fluency and naming. Furthermore, whole-brain ROI-to-ROI connectivity analyses revealed reduced intrahemispheric and right intrahemispheric functional connectivity in ACC patients as compared to controls. In addition, stronger functional connectivity between left and right temporal areas was associated with better language abilities in the ACC group. In healthy controls, no association between language abilities and connectivity was found. Our results show that ACC is associated not only with less interhemispheric, but also with less right intrahemispheric language network connectivity in line with reduced verbal abilities. The present study, thus, supports the excitatory role of the corpus callosum in functional language network connectivity and language abilities.

The role of visual experience for spatial numerical associations

2012 ◽  
Vol 25 (0) ◽  
pp. 222 ◽  
Author(s):  
Michael J. Proulx ◽  
Achille Pasqualotto ◽  
Shuichiro Taya
Keyword(s):  
Visual Experience ◽  
Opposite Effect ◽  
Random Number Generation ◽  
Number Line ◽  
Mental Number Line ◽  
Generation Task ◽  
Congenitally Blind ◽  
Left And Right ◽  
The Right

The topographic representation of space interacts with the mental representation of number. Evidence for such number–space relations have been reported in both synaesthetic and non-synaesthetic participants. Thus far most studies have only examined related effects in sighted participants. For example, the mental number line increases in magnitude from left to right in sighted individuals (Loetscher et al., 2008, Curr. Biol.). What is unclear is whether this association arises from innate mechanisms or requires visual experience early in life to develop in this way. Here we investigated the role of visual experience for the left to right spatial numerical association using a random number generation task in congenitally blind, late blind, and blindfolded sighted participants. Participants orally generated numbers randomly whilst turning their head to the left and right. Sighted participants generated smaller numbers when they turned their head to the left than to the right, consistent with past results. In contrast, congenitally blind participants generated smaller numbers when they turned their head to the right than to the left, exhibiting the opposite effect. The results of the late blind participants showed an intermediate profile between that of the sighted and congenitally blind participants. Visual experience early in life is therefore necessary for the development of the spatial numerical association of the mental number line.

An oligomer complementary to c-myc mRNA inhibits proliferation of HL-60 promyelocytic cells and induces differentiation

1988 ◽  
Vol 8 (2) ◽  
pp. 963-973
Author(s):  
J T Holt ◽  
R L Redner ◽  
A W Nienhuis
Keyword(s):  
Cell Growth ◽  
Protein Concentration ◽  
Myeloid Differentiation ◽  
Sequence Specificity ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Steady State Levels ◽  
Cell Growth And Differentiation ◽  
Antisense Oligomer

To study the role of a nuclear proto-oncogene in the regulation of cell growth and differentiation, we inhibited HL-60 c-myc expression with a complementary antisense oligomer. This oligomer was stable in culture and entered cells, forming an intracellular duplex. Incubation of cells with the anti-myc oligomer decreased the steady-state levels of c-myc protein by 50 to 80%, whereas a control oligomer did not significantly affect the c-myc protein concentration. Direct inhibition of c-myc expression with the anti-myc oligomer was associated with a decreased cell growth rate and an induction of myeloid differentiation. Related antisense oligomers with 2- to 12-base-pair mismatches with c-myc mRNA did not influence HL-60 cells. Thus, the effects of the antisense oligomer exhibited sequence specificity, and furthermore, these effects could be reversed by hybridization competition with another complementary oligomer. Antisense inhibition of a nuclear proto-oncogene apparently bypasses cell surface events in affecting cell proliferation and differentiation.

The cell biology and pathogenic role of pulmonary intravascular macrophages

1990 ◽  
Vol 258 (2) ◽  
pp. L1-L12 ◽  
Author(s):  
A. E. Warner ◽  
J. D. Brain
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Cell Biology ◽  
Morphological Characteristics ◽  
Laboratory Animals ◽  
Capillary Endothelium ◽  
Phagocytic Cells ◽  
Pulmonary Uptake ◽  
Pulmonary Intravascular Macrophages

Pulmonary intravascular macrophages (PIMs) are an extensive population of mature phagocytic cells adherent to the pulmonary capillary endothelium in selected species. They are not prevalent in lungs of commonly studied laboratory animals, such as rodents, and thus have only been recently appreciated. However, their potential role in host defense and acute lung injury has attracted interest, since a number of studies have demonstrated pulmonary localization of circulating particles, microbes, and endotoxin by PIMs. Those animal species, such as ruminants, that provide useful models of pathogen (or endotoxin)-induced acute lung injury demonstrate rapid pulmonary uptake of bacteria by PIMs. Inflammatory mediators released by activated PIMs may initiate the process and provoke accumulation of neutrophils and platelets. This review summarizes the morphological characteristics of PIMs and their species distribution. The role of these members of the mononuclear phagocyte system, both beneficial and potentially pathogenic, is reviewed. The question of whether PIMs have a role in acute lung injury in humans is also discussed.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

Abstract 336: Angiotensinogen Gene Polymorphism Confers a Multiple Risk Factor for Atherosclerosis and Its Susceptibility Traits

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Nduna Dzimiri ◽  
Samar Elhawari ◽  
Mohammed Al-Najai ◽  
Paul Muiya ◽  
Daisy Gueco ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Significant Risk ◽  
Renin Angiotensin System ◽  
Systemic Blood Pressure ◽  
Multiple Risk Factor ◽  
Systemic Blood ◽  
Angiotensin System ◽  
Cardiovascular Functions

Angiotensinogen (AGT) constitutes a pivotal component of the renin-angiotensin system which controls the systemic blood pressure and several other cardiovascular functions. In order to evaluate the role of the AGT gene polymorphism in atherosclerosis (CAD) and its risk factors, we first sequenced the gene in 200 individuals in search of informative SNPs. Of the >100 SNPs identified, we proceeded to evaluate the association of 8 variants in 3232 CAD cases versus 2292 angiographed controls by the Applied Biosystems real-time PCR system. Of the studied variants, the recessive mode of inheritance (TT) of rs3789679C>T [Odds ratio(95% Confidence Interval) = 1.23(1.05-1.45); p=0.012]; TT of rs699C>T [1.21(1.11-1.32); p<0.0001] as well as the dominant models (AG+GG) of rs5051A>G [1.22(1.10-1.37; p<0.0001) and AG+GG of rs2067853A>G [1.23(1.07-140);p=0.003] conferred risk for CAD. Interestingly, rs699 (p<0.0001), rs5051 (p=0.003) and rs2067853 (p=0.001) were equally implicated in hypertension, while the rs699 was further associated with hypercholesterolaemia (p=0.002) and hypertriglyceridaemia (p<0.0001), and the rs5051 was similarly associated with type 2 diabetes mellitus (T2DM) (p=0.011). One 8-mer haplotype from the 8 studied SNPs ACGGATAT (χ 2 =9.83; p=0.0017) and several of its variants, including the 7-mer ACGGATA (χ 2 =9.40; p=0.0022), 6-mer GGATAT (χ 2 =11.54; p=0.0007) and 5-mer GATAT (χ 2 =10.89; p=0.001) conferred significant risk for CAD. Furthermore, three 8-mer haploptypes GTGGGTGG (χ 2 =6.96; p=0.0083), ACGGGTAT (χ 2 =4.70; p=0.030) and GTAGGCGG (χ 2 =5.69; p=0.017) were associated with HTN. Similar trends were also observed for T2DM, obesity and hyperlipidaemia. These observations show that the AGT gene polymorphism confers a common risk for atherosclerosis and its major susceptibility traits.

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