scholarly journals Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sheetal Bhasin ◽  
H. A. Modi
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Glucose Isomerase ◽  
Fermentation Medium ◽  
Potassium Nitrate ◽  
Pure Nitrogen ◽  
Streptomyces Sp ◽  
High Fructose ◽  
Pharmaceutical Industries ◽  
Optimization Of Fermentation

The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of numerous enzymes including glucose isomerase. An array of 75 isolates was screened for the production of glucose isomerase. The isolate Streptomyces sp. SB-P1 was found to produce maximum amount of extracellular GI. Sucrose and raffinose among pure carbon sources and corn cob and wheat husk among crude agro residues were found to yield high enzyme titers. Potassium nitrate among pure nitrogen sources and soy residues among crude sources gave maximum production. Quantitative effect of carbon, nitrogen, and inducer on GI was also determined. Plackett-Burman design was used to study the effect of different medium ingredients. Sucrose and xylose as carbon sources and peptone and soy residues as nitrogen sources proved to be beneficial for GI production.

scholarly journals Optimization of Cellulase Production from Bacteria Isolated from Soil

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Sonia Sethi ◽  
Aparna Datta ◽  
B. Lal Gupta ◽  
Saksham Gupta
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
Pseudomonas Fluorescens ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Optimum Conditions ◽  
E Coli

Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens, Bacillus subtilIs, E. coli, and Serratia marcescens. Optimization of the fermentation medium for maximum cellulase production was carried out. The culture conditions like pH, temperature, carbon sources, and nitrogen sources were optimized. The optimum conditions found for cellulase production were 40°C at pH 10 with glucose as carbon source and ammonium sulphate as nitrogen source, and coconut cake stimulates the production of cellulase. Among bacteria, Pseudomonas fluorescens is the best cellulase producer among the four followed by Bacillus subtilis, E. coli, and Serratia marscens.

scholarly journals Effect of Carbon Sources, Nitrogen Sources and Prebiotics on Growth of Saccharomyces Boulardii

2019 ◽  
Vol 23 (2) ◽  
pp. 101-108
Author(s):  
Xin Yang ◽  
Guowei Shu ◽  
Zhangteng Lei ◽  
Guanli Du ◽  
Zemin Liu ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Gastrointestinal Diseases ◽  
Potassium Nitrate ◽  
Soluble Starch ◽  
Saccharomyces Boulardii ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Dry Cell

Abstract Saccharomyces boulardii (S. boulardii) has been used as a probiotic for the prevention or treatment of various human gastrointestinal diseases for many years. Thus, S. boulardii has a wide range of application prospects in medicine and food industry. The experiments were investigated with effecting of carbon sources (galactose, sucrose, fructose, maltose, lactose, glucose, and soluble starch), nitrogen sources (tryptone, casein, yeast extract, peptone, soy peptone, beef extract and malted milk; ammonium sulfate, urea, diammonium hydrogen citrate, triammonium citrate, ammonium nitrate, ammonium chloride and potassium nitrate), prebiotics (xylo-oligosaccharide, isomaltooligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, stachyose, raffinose and inulin) on the number of viable cells and dry cell weight of S. boulardii. The optimum concentration of 5 % glucose or sucrose, 2 % peptone or yeast extract, 0.4 % urea, 0.2 % fructo-oligosaccharide and 0.6 % galacto-oligosaccharide for S. boulardii grew better in medium.

scholarly journals Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

2021 ◽  
Vol 9 (6) ◽  
pp. 1280
Author(s):  
Dana Byrtusová ◽  
Martin Szotkowski ◽  
Klára Kurowska ◽  
Volha Shapaval ◽  
Ivana Márová
Keyword(s):  
Biomass Yield ◽  
Synthetic Medium ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Potassium Nitrate ◽  
Economic Feasibility ◽  
Intracellular Lipids ◽  
Microbial Biotechnology ◽  
Food And Feed ◽  
Valuable Product

Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain R. kratochvilovae CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans.

scholarly journals Protease Production from UV Mutated Bacillus subtilis

2012 ◽  
Vol 47 (1) ◽  
pp. 69-76 ◽  
Author(s):  
MG Sher ◽  
M Nadeem ◽  
Q Syed ◽  
M Irfan ◽  
S Baig
Keyword(s):  
Bacillus Subtilis ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Maximum Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Protease Enzyme ◽  
Uv Mutation

UV mutation of the strain has significant contributation to enhance the yield of protease enzyme from Bacillus subtilis bacteria under the  cultivation conditions in submerged fermentation. The fermentation medium used for the production of protease composed of carbon  sources 1%, organic 1% or inorganic nitrogen sources 0.5% , K2HPO4 0.2 %, CaCl2 0.04% and MgSO4 0.02 % by mutated Bacillus subtilis  G-4 under the optimum parameters which are important to induce the mutated strain to produce high units of the protease, which were  temperature 37.5°C, pH 9, inoculum size 3 % v/v, glucose 1% as carbon source and peptone 1% as nitrogen source were give the maximum  455.25 + 1.66 units of protease. The results of stability studies revealed that protease of B. subtilis G-4 was stable over a broad range  of temperature (30 to 60°C) and pH (8 to 12). However, maximum activity (155.45U/ml) was observed at temperature 50°C and pH 10.  These characteristics render its potential use in detergent industries for detergent formulation.DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10725 Bangladesh J. Sci. Ind. Res. 47(1), 69-76, 2012

Influence of cultivation conditions on production of lignocellulolytic enzymes by Ceriporiopsis subvermispora

Biologia ◽  
2011 ◽  
Vol 66 (5) ◽  
Author(s):  
Daniela Chmelová ◽  
Miroslav Ondrejovič ◽  
Vladimír Ondáš ◽  
Ernest Šturdík
Keyword(s):  
Time Course ◽  
Extracellular Enzymes ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Potassium Nitrate ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulolytic Enzymes ◽  
Ceriporiopsis Subvermispora ◽  
Carbon And Nitrogen

AbstractThe aim of this work was to make a survey describing factors that influence the production of extracellular enzymes by white-rot fungus Ceriporiopsis subvermispora responsible for the degradation of lignocellulolytic materials. These factors were: carbon sources (glucose, cellulose, hemicellulose, lignin, maltose and starch), nitrogen sources (ammonium sulphate, potassium nitrate, urea, albumin and peptone), pH, temperature and addition of three different concentrations of Cu2+ and Mn2+. The cellulase and xylanase activities were similar in medium with different carbon sources and the highest cellulase and xylanase activities were measured in medium with urea and potassium nitrate as nitrogen sources, respectively. The highest laccase activity was observed in medium with lignin and peptone as carbon and nitrogen sources. In other experiments, time course of production of lignocellulolytic enzymes by white-rot fungus C. subvermispora in medium with lignin or glucose as carbon sources was observed.

scholarly journals Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase fromBacillus megateriumUsing Response Surface Methodology

Scientifica ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Hoang-Yen Thi Nguyen ◽  
Gia-Buu Tran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Bacillus Megaterium ◽  
Large Scale ◽  
Glucose Isomerase ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Medium Components ◽  
High Fructose ◽  
Optimization Of Fermentation

Glucose isomerase is an enzyme widely used in food industry for producing high-fructose corn syrup. Many microbes, includingBacillus megaterium, have been found to be able to produce glucose isomerase. However, the number of studies of glucose isomerase production fromBacillus megateriumis limited. In this study, we establish the optimal medium components and culture conditions forBacillus megateriumglucose isomerase production by evaluating the combined influence of multiple factors and different parameters via Plackett–Burman design and response surface methodology in Modde 5.0 software. The optimized conditions, which were experimentally confirmed as follows: D-xylose (1.116%), K2HPO4(0.2%), MgSO4·7H2O (0.1%), yeast extract (1.161%), peptone (1%), pH 7.0, inoculum size 20% (w/v), shaking 120 rpm at 36.528°C for 48 hours, give rise to production of highest activity of glucose isomerase (0.274 ± 0.003 U/mg biomass). These results provide additional important information for future development of large-scale glucose isomerase production byBacillus megaterium.

Sign in / Sign up

Export Citation Format

Share Document