scholarly journals In VitroEvaluations of Cytotoxicity of Eight Antidiabetic Medicinal Plants and Their Effect on GLUT4 Translocation

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Sleman Kadan ◽  
Bashar Saad ◽  
Yoel Sasson ◽  
Hilal Zaid
Keyword(s):  
Cell Line ◽  
Muscle Cell ◽  
Plant Extracts ◽  
Plasma Membranes ◽  
Nigella Sativa ◽  
Urtica Dioica ◽  
Glut4 Translocation ◽  
Atriplex Halimus ◽  
Islamic Medicine ◽  
Trigonella Foenum Graecum

Despite the enormous achievements in conventional medicine, herbal-based medicines are still a common practice for the treatment of diabetes.Trigonella foenum-graecum,Atriplex halimus,Olea europaea,Urtica dioica,Allium sativum,Allium cepa,Nigella sativa, andCinnamomum cassiaare strongly recommended in the Greco-Arab and Islamic medicine for the treatment and prevention of diabetes. Cytotoxicity (MTT and LDH assays) of the plant extracts was assessed using cells from the liver hepatocellular carcinoma cell line (HepG2) and cells from the rat L6 muscle cell line. The effects of the plant extracts (50% ethanol in water) on glucose transporter-4 (GLUT4) translocation to the plasma membrane was tested in an ELISA test on L6-GLUT4myc cells. Results obtained indicate thatCinnamomon cassiais cytotoxic at concentrations higher than 100 μg/mL, whereas all other tested extracts exhibited cytotoxic effects at concentrations higher than 500 μg/mL. Exposing L6-GLUT4myc muscle cell to extracts fromTrigonella foenum-graecum,Urtica dioica,Atriplex halimus, andCinnamomum verumled to a significant gain in GLUT4 on their plasma membranes at noncytotoxic concentrations as measured with MTT assay and the LDH leakage assay. These findings indicate that the observed anti-diabetic properties of these plants are mediated, at least partially, through regulating GLUT4 translocation.

scholarly journals Diyabette Kullanılan Bitkisel Desteklerin Etkinliği ve Güvenilirliği

2019 ◽  
Vol 47 ◽  
pp. 110-117
Author(s):  
Ezgi Bellikci Koyu
Keyword(s):  
Nigella Sativa ◽  
Zingiber Officinale ◽  
Urtica Dioica ◽  
Momordica Charantia ◽  
Trigonella Foenum Graecum ◽  
Zingiber Officinale Roscoe ◽  
Trigonella Foenum Graecum L

Bitkiler uzun yüzyıllar boyunca tedavinin en önemli parçası olmuşlardır. On dokuzuncu yüzyılda kimya alanında önemli gelişmelerin olması ve ilaç moleküllerinin keşfi ile bitkilerin tıbbi amaçlı kullanımları giderek azalsa da, son yıllarda doğaya dönüş çabaları içerisinde kullanımlarında yeniden bir artış gözlenmektedir. Özellikle diyabet gibi kronik seyirli hastalıklarda modern tedaviye destek olarak bu ürünlerin kullanımları sıklıkla tercih edilmektedir. Bu ilgiye paralel olarak son yıllarda bitkilerin diyabet tedavisindeki etkisini araştıran klinik çalışmalar da artmaya başlamıştır. Etkinliğin yanı sıra bitkilerin güvenilir kullanımları da son derece önemlidir. Doğru bitkinin kullanımı, kullanılan ürünün bileşimi, kalitesi, hazırlama yöntemi, yan etkileri, ilaç etkileşimleri ve kontraendikasyonları da tedavi sürecini etkileyen ve güvenilir kullanım için göz önünde bulundurulması gereken faktörlerdir. Bu derlemede tarçın (Cinnamomum sp.), çörek otu (Nigella sativa L.), kudret narı (Momordica charantia L.), çemen otu (Trigonella foenum-graecum L.), zencefil (Zingiber officinale Roscoe) ve ısırgan otu (Urtica dioica L.) gibi diyabet tedavisinde sıklıkla tercih edilen bitkilerin etkinliği ve güvenilirliği değerlendirilmiştir.

1429: Upregulation of Rho-Kinase in a Human Corpus Cavernosum Smooth Muscle Cell Line in Response to Hypoxia

2004 ◽  
Vol 171 (4S) ◽  
pp. 376-377
Author(s):  
Yongmu Zheng ◽  
Shaohua Chang ◽  
Alan J. Wein ◽  
Samuel Chacko ◽  
Michael E. DiSanto
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Muscle Cell Line

Characterization of a continuous smooth muscle cell line derived from rabbit aorta

1989 ◽  
Vol 25 (10) ◽  
pp. 892-898 ◽  
Author(s):  
Maurice Nachtigal ◽  
Madan L. Nagpal ◽  
Phillip Greenspan ◽  
Sidonia A. Nachtigal ◽  
Alain Legrand
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Rabbit Aorta ◽  
Muscle Cell Line

Chronic and acute ethanol treatment modifies fluidity and composition in plasma membranes of a human hepaic cell line (WRL-68)

1995 ◽  
Vol 11 (2) ◽  
pp. 69-78 ◽  
Author(s):  
M. C. Guti�rrez-Ruiz ◽  
J. L. G�mez ◽  
V. Souza ◽  
L. Bucio
Keyword(s):  
Cell Line ◽  
Plasma Membranes ◽  
Ethanol Treatment ◽  
Acute Ethanol

scholarly journals The hyaluronate synthase from a eukaryotic cell line

1993 ◽  
Vol 290 (3) ◽  
pp. 791-795 ◽  
Author(s):  
L Klewes ◽  
E A Turley ◽  
P Prehm
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Affinity Chromatography ◽  
Cell Line ◽  
Aqueous Phase ◽  
Plasma Membranes ◽  
Eukaryotic Cell ◽  
Molecular Masses ◽  
Triton X ◽  
Hyaluronate Synthase

The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.

scholarly journals Plasma Membrane Effects of Sphingolipid-Synthesis Inhibition by Myriocin in CHO Cells: A Biophysical and Lipidomic Study

2021 ◽  
Author(s):  
Bingen G. Monasterio ◽  
Noemi Jiménez-Rojo ◽  
Aritz B. García-Arribas ◽  
Howard Riezman ◽  
Félix M. Goñi ◽  
...  
Keyword(s):  
Cell Line ◽  
Cho Cells ◽  
Plasma Membranes ◽  
Mechanical Resistance ◽  
Serine Palmitoyltransferase ◽  
Cho Cell ◽  
Cellular Effects ◽  
Inhibitory Compound ◽  
Membrane Effects ◽  
Biophysical Changes

Abstract Two main strategies for establishing the cellular effects of a given enzyme activity suppression are (a) the use of a stably mutated cell line that lacks a functional gene, or (b) treating the wild type with an inhibitory compound that affects the same gene-product protein. In this work, myriocin was used to block the serine palmitoyltransferase (SPT) enzyme of CHO cells and the subsequent biophysical changes in membranes were measured and compared with results obtained with a genetically modified CHO cell line containing a defective SPT (the LY-B cell line). Similar effects were observed with both approaches: sphingomyelin values were markedly decreased in myriocin-treated CHO cells and, in consequence, their membrane molecular order (measured as laurdan general polarization) and mechanical resistance (AFM-measured breakthrough force values) happened to be lower than in the native, non-treated cells. Cells treated with myriocin reacted homeostatically to maintain membrane order, synthesizing more fully saturated and less polyunsaturated glycerophospholipids than the non-treated ones, although they achieved it only partially, their plasma membranes remaining more fluid and less penetrable than those from the control cells.

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