Effect of Prosopis Farcta, Urtica Dioica and Disper root on Vegetative Growth, Nutrients Contents of cv. Shahreban Pomegranate Transplant

This research was conducted on pomegranate transplants in a lath house during the growth season of 2020 of college of Agricultural engineering science, university of Duhok, to investigate the impact of spraying of Prosopis Farcta, Urtica Dioica and Disper root with 0,100, and 200 mg.L-1 concentration, with 0,100, and 200 mg.L-1 concentration and with (0, 75, and 150 mg.L-1) concentration respectively, on vegetative growth, nutrients contents of pomegranate transplants. The collected data indicate that the spraying of Prosopis Farcta extract, Urtica Dioica and Disper root especially at 200, 200, and 150 mg.L-1 respectively resulting in a considerable increase in the majority of the examined parameters. At high concentrations, the interaction of three examined components resulted in the maximum values of branches number 14.57, transplant height 143.67 cm, leaves number 157.33, Chlorophyll 48.97, Roots number 10, Root length 42.67 cm, leaf nitrogen content 2.507 %, phosphorus 0.267 % and potassium 1.433 %.

Substantiation of technological parameters of the oil extract obtained from Urtica dioica roots based on the yield determination of phytosterols using capillary gas chromatography method

Due to the content of phytosterols, extractive preparations of Urtica dioica roots are able to show antiandrogenic effect in the case of external therapy of men and women with androgenic alopecia. Oil extracts (OE) are characterized by several advantages when applied to the skin of the scalp compared to water-alcohol extracts. For the development of OE technology from Urtica dioica roots, it is important to choose the optimal extraction parameters, which are based on the quantitative determination of phytosterols in the extractant and the studied samples of extracts. The aim of the work is to choose the optimal parameters for obtaining OE from Urtica dioica roots based on quantitative determination of phytosterols content in experimental samples of OE by gas capillary chromatography. Materials and methods. Objects of the research – Urtica dioica root, refined corn oil, refined sunflower oil, samples of oil extracts. Determination of phytosterol content in experimental samples was carried out by gas capillary chromatography (chromatograph “Crystal 2000”, manufacturer – research and production company “Analytics”). Results. 5 different compounds of steroid structure (stigmasterol, β-sitosterol, etc.) were identified in sunflower oil by gas liquid chromatography, and 10 (campesterol, 2-α stigmasterol, β-sitosterol, Δ5-avenosterol, etc.) were identified in sunflower oil. The quantitative content of β-sitosterol in the sum of sterols of corn oil was significantly higher compared to the content of this substance in sunflower oil and amounted to 59.33 %. Optimal technological parameters were established considering the peculiarities of extraction with oil extractant and quantitative determination of the amount of phytosterols and β-sitosterol in experimental samples of OE. The total content of plant sterols in OE, including considering their amount in the extractant, was in the range of 7880 mg/kg; the amount of β-sitosterol was 4638 mg/kg. Conclusion. The choice of optimal parameters for obtaining OE from UDR based on determination of phytosterol yield by gas capillary chromatography was experimentally substantiated, namely: extractant – corn oil, raw material-extract ratio – 1: 5, extraction time – 6 h, extraction method – maceration

Observations on the Productivity of Breeding Specimens of Urtica dioica L. from European Russian Ecotopes in Comparison with the Breeding Variety under Field Crop Conditions

Nettle is most often studied as a spinning plant, as a source of biochemicals and environmentally safe fungicides. Major studies are mostly conducted on natural samples and plant populations. Prospects for the use of plant biological resources of stinging nettle (Urtica dioica L.) from the Cretaceous south of the Central Russian Upland to create cultivars for a wide range of applications are considered. The aim of the study is to investigate the productivity of fresh weight and its biochemical composition in the variety of stinging nettle Avicenna, created on the basis of the initial material selected in the Belgorod region of Russia and patented in 2019, and new promising cultivars UD 32/06 and UD 12/16. The experiments were carried out by the split plot method with full randomization in four replicates. The cultivars UD 32/06 and UD 12/16 reliably surpass the Avicenna cultivar in the collection of fresh mass weight by 16.6–22.7% and 23.1 to 27.8%, dry weight by 11.4–28.7% and 1.9–32.7%, and seeds by 19.7–32.0% and 23.2–40.0% accordingly. Analysis of variance showed a significant effect of the factor “Cultivar” on the productive traits “Fresh weight yield” (strength influence 87.2%), “Dry weight yield” (strength influence 43.9%), and “Seed yield” (h2x = 61.6%). The content of crude protein in dry weight of the Avicenna and new varieties is 21.1–24.2%, crude fat: 2.5–4.2%, fiber: 12.0–14.8%. High content of ascorbic acid, calcium, phosphorus, iron, and zinc were observed in all material tested. It is concluded that the biological resources of the wild-growing forms of Urtica dioica L. from the European south of Russia are a valuable source material for obtaining varieties, and varieties with high productivity of the aboveground mass and stable seed productivity. The obtained research results prove the usefulness of nettle cultivation.