scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1521 ◽  
Author(s):  
Yehong Li ◽  
Yiming Zhang ◽  
Zejun Zhang ◽  
Yupiao Hu ◽  
Xiuming Cui ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Evaluation Method ◽  
Herbal Medicines ◽  
Assessment Method ◽  
Gastrodia Elata ◽  
Clinical Safety ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.

scholarly journals EFFECT OF DIFFERENT ANTICOAGULANTS IN DETERMINING METFORMIN HYDROCHLORIDE LEVELS IN HUMAN PLASMA USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 10 (1) ◽  
pp. 148
Author(s):  
Yahdiana Harahap ◽  
Lista Roro Marsudi ◽  
Sunarsih .
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Internal Standard ◽  
Array Detector ◽  
Metformin Hydrochloride ◽  
Significant Difference

Objective: This study aimed to develop and validate an analytical method and to determine the effect of different anticoagulants for analyzingmetformin HCl level in human plasma.Methods: Analysis was performed with high-performance liquid chromatography method using a photodiode array detector set at 234 nm, a C18SunFire™ column (5 μm, 250 mm×4.6 mm), a temperature of 40°C, a mobile phase comprising 10 mM sodium dodecyl sulfate and 10 mM phosphatebuffer in water-acetonitrile (60:40 v/v) at pH 7.0, with a flow rate of 1.0 mL/min, and atorvastatin calcium as the internal standard. Plasmaextraction was performed through protein precipitation using human plasma (300 μL) and acetonitrile (600 μL; 1:2 v/v). The method was linear at aconcentration range of 20.0–5000.0 ng/mL, with r>0.9998.Results: The stability and recovery of metformin HCl in human plasma were not different between citrate with ethylenediaminetetraacetic acid(EDTA) and heparin with EDTA as anticoagulants. However, a significant difference in the peak area response ratio (p<0.05) was observed betweenthe three anticoagulants.Conclusion: The validation results for the three types of plasma anticoagulants met validation requirements based on the European MedicinesAgency bioanalytical guidelines of 2011 for accuracy and precision, selectivity, calibration curve linearity, dilution integrity, carry-over, and stability.

Quantitative and Chemical Fingerprint Analysis of Desmodium styracifolium by High-Performance Liquid Chromatography Combined with Chemometrics

2019 ◽  
Vol 58 (4) ◽  
pp. 294-302
Author(s):  
Liangyuan Chen ◽  
Xiaomin Tang ◽  
Quan Yang ◽  
Xuanxuan Cheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Evaluation Method ◽  
Internal Standard ◽  
Principal Component ◽  
Desmodium Styracifolium ◽  
External Standard Method ◽  
Geographical Regions ◽  
Significant Difference

Abstract In this study, a valid and comprehensive evaluation method for assessing the quality of Desmodium styracifolium (Osb.) Merr has been established, based on analysis of high-performance liquid chromatography fingerprint combined with the similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA), discriminant analysis (DA) and the quantitative analysis multi-components by single marker (QAMS) method. Eleven peaks of the common model were obtained and analyzed using SA, HCA, PCA and DA analysis. These methods indicated a similar conclusion that 31 batches of D. styracifolium samples were categorized into two clusters basically coincident with their geographical regions of origin. Four peaks were identified as schaftoside, isoorientin, isoschaftoside and isovitexin. Schaftoside was selected as the internal standard, and the relative correction factors between schaftoside and the other three flavonoids were calculated using the QAMS method. The accuracy of the QAMS method was verified by comparing with the results calculated by the external standard method. No significant difference between the two methods was found. In conclusion, the established methods were scientifically applied in the quality evaluation of D. styracifolium.

scholarly journals A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Fuchao Chen ◽  
Baoxia Fang ◽  
Peng Li ◽  
Sicen Wang
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Dosage Form ◽  
Potassium Dihydrogen Phosphate ◽  
Dihydrogen Phosphate ◽  
Receptor Antagonists ◽  
Internal Reference ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. Chromatographic separation was achieved using a C18 column (150 mm × 4.6 mm, 5.0 μm), and the mobile phase was composed of acetonitrile-0.05 mol·L−1 potassium dihydrogen phosphate (pH 4.0) (25 : 75) at a flow rate of 1.0 mL·min−1 and detection wavelengths of 307 nm (ondansetron, azasetron, ramosetron), 302 nm (granisetron), and 285 nm (tropisetron). In addition, the accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. The established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals.

scholarly journals Simultaneous Quantitative Analysis of Q-Marker with One Single Reference in Glycyrrhiza uralensis Fisch

2020 ◽  
Vol 58 (6) ◽  
pp. 511-519
Author(s):  
Xin Dong ◽  
Fangyuan Zheng ◽  
Xin Liu ◽  
Lianju Zhang ◽  
Rongqin Hu ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Standard Method ◽  
High Performance ◽  
Glycyrrhiza Uralensis ◽  
Glycyrrhiza Uralensis Fisch ◽  
External Standard Method ◽  
Relative Standard

Abstract In traditional Chinese medicine (TCM) studies, it is difficult to choose evaluation markers for the strict quality control of herbs. A high performance liquid chromatography coupled with metabolomics for simultaneous quantitative analysis of quality markers (Q-markers) in Glycyrrhiza uralensis Fisch was established, which could not only ensure the quality and batch-to-batch consistency of TCMs, but also achieve a quantitative analysis of multi-components by the single reference standard. Based on the construction of chromatographic profiles by high performance liquid chromatography (HPLC) and HPLC-Q-Exactive/MS methods, different multivariate analyses were employed. Seven quantitative indices were selected as the Q-markers, and a reliable quantification method was established. The quantitative method was acceptable with good linearity with correlation coefficients &gt;0.9993 and satisfactory repeatability (relative standard deviation (RSD) &lt; 0.05%), precision (RSD &lt; 0.24%), reproducibility (RSD &lt; 0.97%), stability (RSD &lt; 2.52%) and recoveries (96.96%—98.52%, RSD &lt; 3.24%), and no significant differences were observed between the external standard method and the new method as determined by calculating standard method difference. Overall, the study suggests that the simultaneous quantitative analysis of main Q-marker in G. uralensis Fisch with one single marker can be considered good quality criteria for performing quality control of G. uralensis Fisch.

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