Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity

Nanodiamonds (NDs) have been recognized as emerging carbon-based delivery vehicles due to their biocompatibility. NDs were reported to be nontoxic and suited for biomedical applications in the complete cell culture medium. However, in this study, the cytotoxicity of NDs in serum-free medium was studied and indicated that serum proteins in cell culture medium played significant effect on the toxicity of NDs. Therefore, the interaction mechanism between NDs and a serum protein (human serum albumin, HSA) was first investigated by fluorescence quenching technique and circular dichroism (CD) spectrometry. The results suggest that HSA strongly bonds on the NDs surface to form “protein corona,” which not only prevents the aggregation of NDs and improves its stability but also inhibits the cytotoxicity of NDs. In serum-free medium, NDs exhibited obvious toxicity toward the human lung epithelial cell line (BEAS-2B) and showed concentration-dependent cytotoxicity. In the presence of bovine serum albumin (BSA), which shares structural homology and similar properties with HSA, toxicity of NDs was apparently inhibited. Therefore, the interaction between serum protein and NDs should be considered in the understanding of the biological effects of NDs exposure in biomedical applications.

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Development of a Biodegradable Microcarrier for the Cultivation of Human Adipose Stem Cells (hASCs) with a Defined Xeno- and Serum-Free Medium

Applied Sciences ◽

10.3390/app11030925 ◽

2021 ◽

Vol 11 (3) ◽

pp. 925

Author(s):

Francesco Muoio ◽

Stefano Panella ◽

Matias Lindner ◽

Valentin Jossen ◽

Yves Harder ◽

...

Keyword(s):

Stem Cells ◽

Cell Culture ◽

Culture Medium ◽

Free Cell ◽

Adipose Stem Cells ◽

Cell Culture Medium ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free ◽

Human Adipose Stem Cells

Stirred single-use bioreactors in combination with microcarriers (MCs) have established themselves as a technology that has the potential to meet the demands of current and future cell therapeutic markets. However, most of the published processes have been performed using fetal bovine serum (FBS) containing cell culture medium and non-biocompatible MCs. This approach has two significant drawbacks: firstly, the inevitable potential risks associated with the use of FBS for clinical applications; secondly, non-biocompatible MCs have to be removed from the cell suspension before implantation, requiring a step that causes loss of viable cells and adds further costs and complications. This study aimed to develop a new platform based on a chemically defined xeno- and serum-free cell culture medium and biodegradable MC that can support the growth of human adipose stem cells (hASCs) while still preserving their undifferentiated status. A specific combination of components and manufacturing parameters resulted in a MC prototype, called “BR44”, which delivered the desired functionality. MC BR44 allows the hASCs to stick to its surface and grow in a chemically defined xeno- and serum-free medium (UrSuppe). Although the cells’ expansion rate was not as high as with a commercial non-biodegradable standard MC, those cultured on BR44 maintained a better undifferentiated status in both static and dynamic conditions than those cultured on traditional 2D surfaces.

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Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2006.10.004 ◽

2007 ◽

Vol 40 (1-2) ◽

pp. 98-103 ◽

Cited By ~ 13

Author(s):

Megha S. Even ◽

Chad B. Sandusky ◽

Neal D. Barnard ◽

Jehangir Mistry ◽

Madhur K. Sinha

Keyword(s):

Cell Culture ◽

Monoclonal Antibodies ◽

Human Insulin ◽

Culture Medium ◽

Free Cell ◽

Cell Culture Medium ◽

Serum Free

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Cell surface heparan sulphate and adhesive property of sublines of rat ascites hepatoma AH7974

Journal of Cell Science ◽

10.1242/jcs.90.4.683 ◽

1988 ◽

Vol 90 (4) ◽

pp. 683-689 ◽

Cited By ~ 1

Author(s):

A. Kimura ◽

T. Kawaguchi ◽

T. Ono ◽

A. Sakuma ◽

Y. Yokoya ◽

...

Keyword(s):

Cell Surface ◽

Culture Medium ◽

Heparan Sulphate ◽

Soluble Form ◽

Foetal Calf Serum ◽

Serum Free Medium ◽

Free Medium ◽

Ascites Hepatoma ◽

Serum Free ◽

Rat Ascites Hepatoma

Two variants (74AD and 74FL) established from rat ascites hepatoma AH7974 were examined for the production of glycosaminoglycans in culture. There was no difference between the adhesive (74AD) and the floating (74FL) variants in quantity of glycosaminoglycans produced by their cultivation in minimum essential medium supplemented with 10% foetal calf serum. However, they were distinctly different in the distribution patterns of heparan sulphate. In 74FL, about 70% of total heparan sulphate was found in the culture medium in soluble form, whereas in 74AD, only 7% was found in the medium and the rest was in the cell-substratum complex. In a serum-free medium, 74AD cells grew without adhering to the substratum. After cultivation, more than 90% of total heparan sulphate was found in the cell-associated fractions and the rest in the substratum fractions. No heparan sulphate was detected in the culture medium. On the other hand, 74FL cells released heparan sulphate to the serum-free medium as much as to the serum-containing medium. The increase in amount of heparan sulphate in the culture medium of 74FL cells was supposed to be caused by failure of the cells to deposit heparan sulphate at the cell surface and not caused by increased production. Cell-substratum adhesion mechanisms involving cell surface heparan sulphate (heparan sulphate proteoglycan) and some serum intermediate(s) are discussed for 74AD cells.

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Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical alpha-minimal essential medium (MEM)

European Journal Of Haematology ◽

10.1111/j.1600-0609.2006.00795.x ◽

2007 ◽

Vol 78 (2) ◽

pp. 168-168 ◽

Cited By ~ 1

Author(s):

N. Meuleman ◽

T. Tondreau ◽

D. Bron ◽

L. Lagneaux

Keyword(s):

Cell Culture ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Serum Free Medium ◽

Free Medium ◽

Minimal Essential Medium ◽

Mesenchymal Stem Cell Culture ◽

Stem Cell Culture ◽

Serum Free

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Cell culture medium, serum-free

Cold Spring Harbor Protocols ◽

10.1101/pdb.rec11256 ◽

2007 ◽

Vol 2007 (23) ◽

pp. pdb.rec11256-pdb.rec11256

Keyword(s):

Cell Culture ◽

Culture Medium ◽

Cell Culture Medium ◽

Serum Free

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Stimulierung von Kulturen embryonaler Rattenzellen durch eine Proteinfraktion aus fötalem Kälberserum / Stimulation of Embryonic Rat Cells in Culture by a Protein Fraction Isolated from Fetal Calf Serum

Zeitschrift für Naturforschung B ◽

10.1515/znb-1971-1018 ◽

1971 ◽

Vol 26 (10) ◽

pp. 1045-1048 ◽

Cited By ~ 34

Author(s):

Dieter F. Hülser ◽

Werner Frank

Keyword(s):

Cell Cycle ◽

Culture Medium ◽

Fetal Calf Serum ◽

Surface Membrane ◽

Calf Serum ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free ◽

Membrane Potential Difference ◽

Stimulation Of

Normal embryonic rat cells incubated in serum-free medium accumulate in G1-phase of the cell cycle. On addition of a growth-stimulating protein isolated from fetal calf serum they are triggered to proceed through the cycle, and they resume DNA-synthesis 15 to 20 hours later. In this paper it is demonstrated that the surface membrane potential difference (PD) decreases immediately after changing serum-free medium against culture medium containing either calf serum or the isolated serum protein; the original PD is restored 2 to 3 hours later. Serumprotein without growthstimulating activity does not affect the PD.A permanent rat cell line which grows independently of serum also has been tested. The PD of these cells is not significantly influenced by calf serum.

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