Preferential Cytotoxic Effect of Genistein on G361 Melanoma Cells Via Inhibition of the Expression of Focal Adhesion Kinase

2012 ◽  
Vol 37 (4) ◽  
pp. 189-195
Author(s):  
Gyoo Cheon Kim ◽  
◽  
Sang Rye Park ◽  
Hyun-Ho Kwak ◽  
Bong-Soo Park
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Cytotoxic Effect ◽  
Melanoma Cells

Geraniin-mediated apoptosis by cleavage of focal adhesion kinase through up-regulation of Fas ligand expression in human melanoma cells

2008 ◽  
Vol 52 (6) ◽  
pp. 655-663 ◽  
Author(s):  
Jang-Chang Lee ◽  
Chih-Yen Tsai ◽  
Jung-Yie Kao ◽  
Ming-Ching Kao ◽  
Shih-Chang Tsai ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Fas Ligand ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
Ligand Expression

scholarly journals Triptolide-Mediated Apoptosis by Suppression of Focal Adhesion Kinase through Extrinsic and Intrinsic Pathways in Human Melanoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10
Author(s):  
Haw-Young Kwon ◽  
Kyoung-Sook Kim ◽  
Ji-Sue Baik ◽  
Hyung-In Moon ◽  
Ji-Won Lee ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Caspase 3 ◽  
Human Cancer ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Caspase 8 ◽  
Cytochrome C Release ◽  
Human Melanoma Cells ◽  
Induced Apoptosis

Triptolide (TPL) has been shown to inhibit cell proliferation and induce apoptosis in various human cancer cells; however, the precise mechanism of apoptosis induced by TPL in human melanoma cells has not yet been elucidated. In this study, we investigated the precise mechanism underlying cytocidal effects of TPL on human melanoma cells. Treatment of human melanoma cells with TPL significantly inhibited cell growth and induced apoptosis, as evidenced by flow cytometry and annexin V-fluorescein isothiocyanate analyses. TPL increased the levels of Fas and Fas-associated death domain (FADD) and induced cleavage of Bid by activation of caspase-8 and cytochrome c release from mitochondria to the cytosol, which resulted in activation of caspase-9 and caspase-3. Moreover, TPL-induced apoptosis in SK-MEL-2 cells was mediated through dephosphorylation of focal adhesion kinase (FAK) and its cleavage by caspase-8-mediated caspase-3 activation via upregulation of Fas expression. We also found that TPL mediated the dissociation of receptor-interacting protein (RIP) from FAK and enhanced the formation of RIP/Fas complex formation initiating cell death. In conclusion, our data firstly demonstrated that TPL induces apoptosis by both extrinsic and intrinsic apoptosis pathways in human melanoma cells and identified that RIP shuttles between Fas and FAK to mediate apoptosis.

scholarly journals Knockdown of proteolipid protein 2 or focal adhesion kinase with an artificial microRNA reduces growth and metastasis of B16BL6 melanoma cells

2011 ◽  
Vol 3 (1) ◽  
pp. 19-24 ◽  
Author(s):  
HIROKI OZAWA ◽  
YOSHIKO SONODA ◽  
TAKAHARU SUZUKI ◽  
NAOMI YOSHIDA-HOSHINA ◽  
MEGUMI FUNAKOSHI-TAGO ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Proteolipid Protein ◽  
Melanoma Cells ◽  
Artificial Microrna

Doxycycline inhibits the adhesion and migration of melanoma cells by inhibiting the expression and phosphorylation of focal adhesion kinase (FAK)

2009 ◽  
Vol 285 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Tao Sun ◽  
Nan Zhao ◽  
Chun-sheng Ni ◽  
Xiu-lan Zhao ◽  
Wen-zhi Zhang ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Melanoma Cells ◽  
And Migration

Early signalling events of autophagy

2013 ◽  
Vol 55 ◽  
pp. 1-15 ◽  
Author(s):  
Laura E. Gallagher ◽  
Edmond Y.W. Chan
Keyword(s):  
Protein Kinase ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Mammalian Cells ◽  
Mammalian Target Of Rapamycin ◽  
Interacting Protein ◽  
The Core ◽  
Autophagy Gene ◽  
Autophagy Induction ◽  
Cellular Pathways

Autophagy is a conserved cellular degradative process important for cellular homoeostasis and survival. An early committal step during the initiation of autophagy requires the actions of a protein kinase called ATG1 (autophagy gene 1). In mammalian cells, ATG1 is represented by ULK1 (uncoordinated-51-like kinase 1), which relies on its essential regulatory cofactors mATG13, FIP200 (focal adhesion kinase family-interacting protein 200 kDa) and ATG101. Much evidence indicates that mTORC1 [mechanistic (also known as mammalian) target of rapamycin complex 1] signals downstream to the ULK1 complex to negatively regulate autophagy. In this chapter, we discuss our understanding on how the mTORC1–ULK1 signalling axis drives the initial steps of autophagy induction. We conclude with a summary of our growing appreciation of the additional cellular pathways that interconnect with the core mTORC1–ULK1 signalling module.

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