Restraining small decapods and amphipods for in vivo laboratory studies

Crustaceana ◽  
2018 ◽  
Vol 91 (5) ◽  
pp. 517-525 ◽  
Author(s):  
Anton Gurkov ◽  
Ekaterina Borvinskaya ◽  
Ekaterina Shchapova ◽  
Maxim Timofeyev
Keyword(s):  
In Vivo Imaging ◽  
Small Animal ◽  
Laboratory Studies ◽  
Advantages And Disadvantages ◽  
Freely Moving ◽  
Physiological Studies

Abstract During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail.

scholarly journals In Vivo Imaging of Peripheral Benzodiazepine Receptors in Mouse Lungs: A Biomarker of Inflammation

2005 ◽  
Vol 4 (4) ◽  
pp. 7290.2005.05133 ◽  
Author(s):  
Matthew J. Hardwick ◽  
Ming-Kai Chen ◽  
Kwamena Baidoo ◽  
Martin G. Pomper ◽  
Tomás R. Guilarte
Keyword(s):  
In Vivo Imaging ◽  
Ex Vivo ◽  
Inflammatory Diseases ◽  
Imaging Techniques ◽  
Benzodiazepine Receptors ◽  
Small Animal ◽  
Small Animal Pet ◽  
Planar Imaging ◽  
Peripheral Benzodiazepine Receptors

The ability to visualize the immune response with radioligands targeted to immune cells will enhance our understanding of cellular responses in inflammatory diseases. Peripheral benzodiazepine receptors (PBR) are present in monocytes and neutrophils as well as in lung tissue. We used lipopolysaccharide (LPS) as a model of inflammation to assess whether the PBR could be used as a noninvasive marker of inflammation in the lungs. Planar imaging of mice administrated 10 or 30 mg/kg LPS showed increased [123I]-( R)-PK11195 radioactivity in the thorax 2 days after LPS treatment relative to control. Following imaging, lungs from control and LPS-treated mice were harvested for ex vivo gamma counting and showed significantly increased radioactivity above control levels. The specificity of the PBR response was determined using a blocking dose of nonradioactive PK11195 given 30 min prior to radiotracer injection. Static planar images of the thorax of nonradioactive PK11195 pretreated animals showed a significantly lower level of radiotracer accumulation in control and in LPS-treated animals ( p < .05). These data show that LPS induces specific increases in PBR ligand binding in the lungs. We also used in vivo small-animal PET studies to demonstrate increased [11C]-( R)-PK11195 accumulation in the lungs of LPS-treated mice. This study suggests that measuring PBR expression using in vivo imaging techniques may be a useful biomarker to image lung inflammation.

NEMA NU 4-2008 and in vivo imaging performance of RAYCAN trans-PET/CT X5 small animal imaging system

2019 ◽  
Vol 64 (11) ◽  
pp. 115014 ◽  
Author(s):  
J Teuho ◽  
C Han ◽  
L Riehakainen ◽  
A Honkaniemi ◽  
M Tirri ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Imaging System ◽  
Small Animal Imaging ◽  
Small Animal ◽  
Animal Imaging ◽  
Pet Ct ◽  
Imaging Performance

Implementation of 89Zr production and in vivo imaging of B-cells in mice with 89Zr-labeled anti-B-cell antibodies by small animal PET/CT

2011 ◽  
Vol 69 (6) ◽  
pp. 852-857 ◽  
Author(s):  
Martin Walther ◽  
Peter Gebhardt ◽  
Philipp Grosse-Gehling ◽  
Lydia Würbach ◽  
Ingo Irmler ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
In Vivo Imaging ◽  
Small Animal ◽  
Small Animal Pet ◽  
Animal Pet ◽  
Pet Ct

Carbon-11 Labeled Tracers for In Vivo Imaging of P-Glycoprotein Function: Kinetics, Advantages and Disadvantages

2010 ◽  
Vol 10 (17) ◽  
pp. 1820-1833 ◽  
Author(s):  
Gert Luurtsema ◽  
Joost Verbeek ◽  
Mark Lubberink ◽  
Adriaan A. Lammertsma ◽  
Rudi Dierckx ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Advantages And Disadvantages ◽  
P Glycoprotein

Basics of Small Animal Handling for In Vivo Imaging

2008 ◽  
pp. 377-390
Author(s):  
Laurence W. Hedlund ◽  
Tracy L. Gluckman
Keyword(s):  
In Vivo Imaging ◽  
Small Animal ◽  
Animal Handling
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