Generation And Characterization Of An Nkx2-1-GFP Reporter Mouse

Author(s):  
Laertis Ikonomou ◽  
Jyh-Chang Jean ◽  
Letty Kwok ◽  
Tyler Longmire ◽  
Darrell N. Kotton
Keyword(s):  
2005 ◽  
Vol 22 (7) ◽  
pp. 1555-1569 ◽  
Author(s):  
Perrine Barraud ◽  
Lachlan Thompson ◽  
Deniz Kirik ◽  
Anders Björklund ◽  
Malin Parmar

2019 ◽  
Author(s):  
Aurélie Bouteau ◽  
Botond Z. Igyártó

AbstractHuLangerin-Cre-YFPf/f mice were generated to specifically mark a subset of antigen presenting immune cells, called Langerhans cells (LCs). During histological characterization of these mice, we found that, in addition to LCs an uncharacterized cell population in the central nervous system (CNS) also expressed YFP. In this study, we found that the CNS YFP+ cells were negative for microglia and astrocyte markers, but they expressed mature neuronal marker NeuN and showed neuronal localization/morphology. Thus, these mice might be used to study the ontogeny, migration and the role of a subset of CNS neurons.


2001 ◽  
Vol 106 (4) ◽  
pp. 251-253 ◽  
Author(s):  
Jae Y. Cho ◽  
T. Dawn Grant ◽  
Gregory P. Lunstrum ◽  
William A. Horton

2006 ◽  
Vol 20 (7) ◽  
pp. 1027-1028 ◽  
Author(s):  
Kristina Lindsten ◽  
Victoria Menendez‐Benito ◽  
Maria G. Masucci ◽  
Nico P. Dantuma ◽  
Asangi R. K. Kumarapeli ◽  
...  

2004 ◽  
Vol 324 (2) ◽  
pp. 795-800 ◽  
Author(s):  
Cameron E. Forde ◽  
Jennifer M. Rocco ◽  
J. Patrick Fitch ◽  
Sandra L. McCutchen-Maloney

2016 ◽  
Vol 92 (5) ◽  
pp. 249-256 ◽  
Author(s):  
Angelo Porciuncula ◽  
Anujith Kumar ◽  
Saray Rodriguez ◽  
Maher Atari ◽  
Miriam Araña ◽  
...  

genesis ◽  
2001 ◽  
Vol 30 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Rainer Constien ◽  
Anne Forde ◽  
Birgit Liliensiek ◽  
Hermann-Josef Gröne ◽  
Peter Nawroth ◽  
...  

2016 ◽  
Author(s):  
Viola Vankova Hausnerova ◽  
Pavel Krizek ◽  
Guy M. Hagen ◽  
Christian Lanctot

The pulsatile nature of transcription has recently emerged as an important property of gene expression. Here we report on the characterization of a RNA polymerase II transgene that is transcribed in the nucleolus. Using the MS2-GFP reporter system and live cell imaging, we found that the synthesis of a MS2-tagged transcript in the nucleolus was discontinuous in all of the cells that were observed, with periods of activity lasting from 15 minutes to 21 hours. The frequency of pulse lengths could be fitted with an exponential function, from which we determined that transcription occurs on average for periods of 20 minutes. These ON periods alternate with periods of inactivity which last on average 29 minutes. The post-mitotic re-activation of transcription was found to be asynchronous in daughter cell pairs. Our observation of discontinuous transcriptional activity in the nucleolus may reflect cycling in the assembly and disassembly of active chromatin structure in and/or around the rDNA genes.


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