Electrospun Nanofibers: From Filtration Membranes to Highly Specialized Tissue Engineering Scaffolds

2014 ◽  
Vol 14 (1) ◽  
pp. 522-534 ◽  
Author(s):  
Vahid Shabafrooz ◽  
Masoud Mozafari ◽  
Daryoosh Vashaee ◽  
Lobat Tayebi
Keyword(s):  
Tissue Engineering ◽  
Electrospun Nanofibers ◽  
Tissue Engineering Scaffolds ◽  
Filtration Membranes ◽  
Specialized Tissue

Programmable Micropatterning of Polymer Nanofibers

2010 ◽  
Author(s):  
Bongsu Kim ◽  
Yi Zhao
Keyword(s):  
Tissue Engineering ◽  
Electrostatic Force ◽  
Electrospun Nanofibers ◽  
Cost Effective ◽  
Experimental Result ◽  
Polymer Nanofibers ◽  
Tissue Engineering Scaffolds ◽  
Effective Manner ◽  
The Difference ◽  
On Chip

This paper reports programmable micropatterning of electrospun nanofibrous materials using a collector chip that consists of an array of independently controllable microelectrodes. The microelectrodes on the collecting chip are prepared by standard photolithography. By programming the local electrical field using excited and floating electrodes, the collector chip allows patterning of microstructures with controllable characteristics. The difference of electrostatic force between the excited and the floating electrodes increases the patterning contrast of electrospun nanofibers. The arbitrary geometries are successfully patterned on the array of 6 × 6 electrodes by independently programmable control of the voltage of each electrode. The experimental result also shows that it is possible to control the porosity and alignment of fibers. This method provides a simple yet highly reliable approach for creating combined micro/nanostructures of polymer nanofibers in a cost effective manner, which has great potential in functional tissue engineering, filtration, and chemical sensing. The work is also expected to foster the use of nanofibers in microdevices for on-chip biochemical analysis, and controlled infiltration and proliferation. The resulting nanofibers with controllable porosity are especially useful for the construction of tissue engineering scaffolds with morphological and functional similarity with natural tissues.

The design trend in tissue-engineering scaffolds based on nanomechanical properties of individual electrospun nanofibers

2013 ◽  
Vol 455 (1-2) ◽  
pp. 338-347 ◽  
Author(s):  
Biljana Janković ◽  
Jan Pelipenko ◽  
Miha Škarabot ◽  
Igor Muševič ◽  
Julijana Kristl
Keyword(s):  
Tissue Engineering ◽  
Electrospun Nanofibers ◽  
Tissue Engineering Scaffolds ◽  
Nanomechanical Properties

scholarly journals 3D Electrospun Nanofiber-Based Scaffolds: From Preparations and Properties to Tissue Regeneration Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-22
Author(s):  
Shanshan Han ◽  
Kexin Nie ◽  
Jingchao Li ◽  
Qingqing Sun ◽  
Xiaofeng Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Porous Matrix ◽  
Electrospun Nanofiber ◽  
Tissue Engineering Scaffolds ◽  
3D Scaffolds

Electrospun nanofibers have been frequently used for tissue engineering due to their morphological similarities with the extracellular matrix (ECM) and tunable chemical and physical properties for regulating cell behaviors and functions. However, most of the existing electrospun nanofibers have a closely packed two-dimensional (2D) membrane with the intrinsic shortcomings of limited cellular infiltration, restricted nutrition diffusion, and unsatisfied thickness. Three-dimensional (3D) electrospun nanofiber-based scaffolds can provide stem cells with 3D microenvironments and biomimetic fibrous structures. Thus, they have been demonstrated to be good candidates for in vivo repair of different tissues. This review summarizes the recent developments in 3D electrospun nanofiber-based scaffolds (ENF-S) for tissue engineering. Three types of 3D ENF-S fabricated using different approaches classified into electrospun nanofiber 3D scaffolds, electrospun nanofiber/hydrogel composite 3D scaffolds, and electrospun nanofiber/porous matrix composite 3D scaffolds are discussed. New functions for these 3D ENF-S and properties, such as facilitated cell infiltration, 3D fibrous architecture, enhanced mechanical properties, and tunable degradability, meeting the requirements of tissue engineering scaffolds were discovered. The applications of 3D ENF-S in cartilage, bone, tendon, ligament, skeletal muscle, nerve, and cardiac tissue regeneration are then presented with a discussion of current challenges and future directions. Finally, we give summaries and future perspectives of 3D ENF-S in tissue engineering and clinical transformation.

Novel tissue engineering scaffolds as wound dressings loaded with Alkannins/Shikonins as active ingredients

2019 ◽  
Author(s):  
AS Arampatzis ◽  
K Theodoridis ◽  
E Aggelidou ◽  
KN Kontogiannopoulos ◽  
I Tsivintzelis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Dressings ◽  
Active Ingredients ◽  
Tissue Engineering Scaffolds

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

scholarly journals Decellularized Porcine Brain Matrix for Cell Culture and Tissue Engineering Scaffolds

2011 ◽  
Vol 17 (21-22) ◽  
pp. 2583-2592 ◽  
Author(s):  
Jessica A. DeQuach ◽  
Shauna H. Yuan ◽  
Lawrence S.B. Goldstein ◽  
Karen L. Christman
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Tissue Engineering Scaffolds ◽  
Porcine Brain

scholarly journals GAKTpore: Stereological Characterisation Methods for Porous Foams in Biomedical Applications

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1269
Author(s):  
Gareth Sheppard ◽  
Karl Tassenberg ◽  
Bogdan Nenchev ◽  
Joel Strickland ◽  
Ramy Mesalam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Large Scale ◽  
Collagen Scaffold ◽  
Porous Structures ◽  
Tissue Engineering Scaffolds ◽  
Biological Responses ◽  
Sem Micrograph ◽  
Cell Adhesion And Proliferation ◽  
Characterisation Methods

In tissue engineering, scaffolds are a key component that possess a highly elaborate pore structure. Careful characterisation of such porous structures enables the prediction of a variety of large-scale biological responses. In this work, a rapid, efficient, and accurate methodology for 2D bulk porous structure analysis is proposed. The algorithm, “GAKTpore”, creates a morphology map allowing quantification and visualisation of spatial feature variation. The software achieves 99.6% and 99.1% mean accuracy for pore diameter and shape factor identification, respectively. There are two main algorithm novelties within this work: (1) feature-dependant homogeneity map; (2) a new waviness function providing insights into the convexity/concavity of pores, important for understanding the influence on cell adhesion and proliferation. The algorithm is applied to foam structures, providing a full characterisation of a 10 mm diameter SEM micrograph (14,784 × 14,915 px) with 190,249 pores in ~9 min and has elucidated new insights into collagen scaffold formation by relating microstructural formation to the bulk formation environment. This novel porosity characterisation algorithm demonstrates its versatility, where accuracy, repeatability, and time are paramount. Thus, GAKTpore offers enormous potential to optimise and enhance scaffolds within tissue engineering.

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