Semiconducting Polymer Dot-Based Ratiometric Fluorescence Nanoprobe for DNA Detection

2021 ◽  
Vol 21 (11) ◽  
pp. 5776-5783
Author(s):  
Zhen-Hu Gong ◽  
Zong-Nan Wei ◽  
Yi-Zhang Liu ◽  
Lu-Fei Xiao
Keyword(s):  
Methylene Blue ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
Deoxyribonucleic Acid ◽  
Experimental Conditions ◽  
Semiconducting Polymer ◽  
Electron Transfer Mechanism ◽  
Fluorescence Nanoprobe ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

A ratiometric fluorescent deoxyribonucleic acid probe was synthesized using the photoinduced electron transfer mechanism, involving the interactions between different fluorescent components and electron acceptors. Double-emission carboxyl functionalized semiconducting polymer dots were synthesized using the nanoprecipitation method and applied as the flurophore, while methylene blue was used as the electron acceptor. Photoinduced electron transfer between different polymer dots components and methylene blue can achieve ratiometric modulation of the overall fluorescence in the system. The addition of deoxyribonucleic acid restores the fluorescence intensity, because the stronger interactions between deoxyribonucleic acid and methylene blue results in methylene blue being separated from the polymer dots. Under the optimized experimental conditions, system fluorescence was restored to its maximum when the concentration of deoxyribonucleic acid reached 200 nM, the linear range was 0.006-200 nM (R2 = 0.995). This probe was reasonably free from interference, showing a good response to deoxyribonucleic acid, with strong application data from actual samples.

Semiconducting Polymer Dots-methylene Blue Fluorescence Energy Transfer System for Determination of Bi(Ⅲ) Ions in Water

2017 ◽  
Vol 38 (10) ◽  
pp. 1353-1358
Author(s):  
刘义章 LIU Yi-zhang ◽  
孙军勇 SUN Jun-yong ◽  
王 磊 WANG Lei ◽  
周 凯 ZHOU Kai ◽  
巩振虎 GONG Zhen-hu
Keyword(s):  
Methylene Blue ◽  
Energy Transfer ◽  
Fluorescence Energy Transfer ◽  
Transfer System ◽  
Blue Fluorescence ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots ◽  
Fluorescence Energy

scholarly journals Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers

Sensors ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 589 ◽  
Author(s):  
Yizhang Liu ◽  
Xuekai Jiang ◽  
Wenfeng Cao ◽  
Junyong Sun ◽  
Feng Gao
Keyword(s):  
Energy Transfer ◽  
Detection Limit ◽  
Human Serum ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Experimental Conditions ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Nanoprecipitation Method ◽  
Semiconducting Polymer Dots

Carboxyl-functionalized semiconducting polymer dots (Pdots) were synthesized as an energy donor by the nanoprecipitation method. A black hole quenching dye (BHQ-labelled thrombin aptamers) was used as the energy acceptor, and fluorescence resonance energy transfer between the aptamers and Pdots was used for fluorescence quenching of the Pdots. The addition of thrombin restored the fluorescence intensity. Under the optimized experimental conditions, the fluorescence of the system was restored to the maximum when the concentration of thrombin reached 130 nM, with a linear range of 0–50 nM (R2 = 0.990) and a detection limit of 0.33 nM. This sensor was less disturbed by impurities, showing good specificity and signal response to thrombin, with good application in actual samples. The detection of human serum showed good linearity in the range of 0–30 nM (R2 = 0.997), with a detection limit of 0.56 nM and a recovery rate of 96.2–104.1%, indicating that this fluorescence sensor can be used for the detection of thrombin content in human serum.

Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots

2021 ◽  
Author(s):  
Haobin Chen ◽  
Jiangbo Yu ◽  
Xiaoxiao Men ◽  
Jicheng Zhang ◽  
Zhaoyang Ding ◽  
...  
Keyword(s):  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

scholarly journals Light-induced crosslinkable semiconducting polymer dots

2015 ◽  
Vol 6 (3) ◽  
pp. 2102-2109 ◽  
Author(s):  
Yue Zhang ◽  
Fangmao Ye ◽  
Wei Sun ◽  
Jiangbo Yu ◽  
I-Che Wu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Chemical Stability ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots ◽  
Physical And Chemical

This paper describes photocrosslinkable Pdots with enhanced colloidal, physical, and chemical stability, and excellent encapsulating ability of functional small molecules.

scholarly journals Optically Encoded Semiconducting Polymer Dots with Single-Wavelength Excitation for Barcoding and Tracking of Single Cells

2017 ◽  
Vol 89 (11) ◽  
pp. 6232-6238 ◽  
Author(s):  
Chun-Ting Kuo ◽  
Hong-Shang Peng ◽  
Yu Rong ◽  
Jiangbo Yu ◽  
Wei Sun ◽  
...  
Keyword(s):  
Single Cells ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

scholarly journals Single-Chain Semiconducting Polymer Dots

Langmuir ◽  
2014 ◽  
Vol 31 (1) ◽  
pp. 499-505 ◽  
Author(s):  
Fangmao Ye ◽  
Wei Sun ◽  
Yue Zhang ◽  
Changfeng Wu ◽  
Xuanjun Zhang ◽  
...  
Keyword(s):  
Single Chain ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

Novel anthracene- and pyridine-containing Schiff base probe for selective “off–on” fluorescent determination of Cu2+ ions towards live cell application

2016 ◽  
Vol 40 (7) ◽  
pp. 6101-6108 ◽  
Author(s):  
Turibius Simon ◽  
Muthaiah Shellaiah ◽  
Venkatesan Srinivasadesikan ◽  
Ching-Chang Lin ◽  
Fu-Hsiang Ko ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Schiff Base ◽  
Photoinduced Electron Transfer ◽  
Transfer Mechanism ◽  
Live Cell ◽  
Live Cells ◽  
Electron Transfer Mechanism

A simple anthracene-based AP probe was synthesized to detect Cu2+ ions, via the photoinduced electron transfer mechanism, in live cells.

Size-Dependent Property and Cell Labeling of Semiconducting Polymer Dots

2014 ◽  
Vol 6 (13) ◽  
pp. 10802-10812 ◽  
Author(s):  
Kai Sun ◽  
Haobin Chen ◽  
Lei Wang ◽  
Shengyan Yin ◽  
Haiyu Wang ◽  
...  
Keyword(s):  
Cell Labeling ◽  
Semiconducting Polymer ◽  
Size Dependent ◽  
Polymer Dots ◽  
Dependent Property ◽  
Semiconducting Polymer Dots
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