scholarly journals Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers

Sensors ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 589 ◽  
Author(s):  
Yizhang Liu ◽  
Xuekai Jiang ◽  
Wenfeng Cao ◽  
Junyong Sun ◽  
Feng Gao
Keyword(s):  
Energy Transfer ◽  
Detection Limit ◽  
Human Serum ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Experimental Conditions ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Nanoprecipitation Method ◽  
Semiconducting Polymer Dots

Carboxyl-functionalized semiconducting polymer dots (Pdots) were synthesized as an energy donor by the nanoprecipitation method. A black hole quenching dye (BHQ-labelled thrombin aptamers) was used as the energy acceptor, and fluorescence resonance energy transfer between the aptamers and Pdots was used for fluorescence quenching of the Pdots. The addition of thrombin restored the fluorescence intensity. Under the optimized experimental conditions, the fluorescence of the system was restored to the maximum when the concentration of thrombin reached 130 nM, with a linear range of 0–50 nM (R2 = 0.990) and a detection limit of 0.33 nM. This sensor was less disturbed by impurities, showing good specificity and signal response to thrombin, with good application in actual samples. The detection of human serum showed good linearity in the range of 0–30 nM (R2 = 0.997), with a detection limit of 0.56 nM and a recovery rate of 96.2–104.1%, indicating that this fluorescence sensor can be used for the detection of thrombin content in human serum.

scholarly journals Remote whispering metamaterial for non-radiative transceiving of ultra-weak sound

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jin Zhang ◽  
Wei Rui ◽  
Chengrong Ma ◽  
Ying Cheng ◽  
Xiaojun Liu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Detection Limit ◽  
Free Space ◽  
Signal To Noise Ratio ◽  
Resonance Energy Transfer ◽  
Information Leakage ◽  
Resonance Energy ◽  
Acoustic Analogy ◽  
Signal To Noise ◽  
Airborne Sound

AbstractTransceiving ultra-weak sound typically relies on signal pre-amplification at the transmitting end via active electro-acoustic devices, which inherently perturbs the environment in the form of noise that inevitably leads to information leakage. Here we demonstrate a passive remote-whispering metamaterial (RWM) enabling weak airborne sound at audible frequencies to reach unprecedented signal enhancement without altering the detected ambient soundscape, which is based on the extraordinary scattering properties of a metamaterial formed by a pair of self-resonating subwavelength Mie meta-cavities, constituting the acoustic analogy of Förster resonance energy transfer. We demonstrate efficient non-radiative sound transfer over distances hundreds times longer than the radius of the meta-cavities, which enables the RWM to recover weak sound signals completely overwhelmed by strong noise with enhanced signal-to-noise ratio from −3 dB below the detection limit of 0 dB in free space to 17.7 dB.

A paper-supported sandwich immunosensor based on upconversion luminescence resonance energy transfer for the visual and quantitative determination of a cancer biomarker in human serum

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4181-4187 ◽  
Author(s):  
Mengyuan He ◽  
Ning Shang ◽  
Lin Shen ◽  
Zhihong Liu
Keyword(s):  
Energy Transfer ◽  
Quantitative Determination ◽  
Human Serum ◽  
Upconversion Luminescence ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cancer Biomarker

A portable and sensitive paper-supported sandwich immunosensor based on UC-FRET was developed for the visual and quantitative determination of CEA.

scholarly journals Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

2019 ◽  
Vol 10 (28) ◽  
pp. 6815-6820 ◽  
Author(s):  
Ningning Wang ◽  
Ziyu Wang ◽  
Lizhen Chen ◽  
Weiwei Chen ◽  
Yiwu Quan ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Transfer Process ◽  
Energy Transfer Process ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Highly Sensitive ◽  
Polymer Dots ◽  
Dual Resonance

A dual intramolecular electrochemiluminescence resonance energy transfer process is proposed with triple-component Pdots to enhance ECL emission, which greatly improves the ECL efficiency and can be used for sensitive and specific visual quantification of different targets.

Semiconducting Polymer Dot-Based Ratiometric Fluorescence Nanoprobe for DNA Detection

2021 ◽  
Vol 21 (11) ◽  
pp. 5776-5783
Author(s):  
Zhen-Hu Gong ◽  
Zong-Nan Wei ◽  
Yi-Zhang Liu ◽  
Lu-Fei Xiao
Keyword(s):  
Methylene Blue ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
Deoxyribonucleic Acid ◽  
Experimental Conditions ◽  
Semiconducting Polymer ◽  
Electron Transfer Mechanism ◽  
Fluorescence Nanoprobe ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

A ratiometric fluorescent deoxyribonucleic acid probe was synthesized using the photoinduced electron transfer mechanism, involving the interactions between different fluorescent components and electron acceptors. Double-emission carboxyl functionalized semiconducting polymer dots were synthesized using the nanoprecipitation method and applied as the flurophore, while methylene blue was used as the electron acceptor. Photoinduced electron transfer between different polymer dots components and methylene blue can achieve ratiometric modulation of the overall fluorescence in the system. The addition of deoxyribonucleic acid restores the fluorescence intensity, because the stronger interactions between deoxyribonucleic acid and methylene blue results in methylene blue being separated from the polymer dots. Under the optimized experimental conditions, system fluorescence was restored to its maximum when the concentration of deoxyribonucleic acid reached 200 nM, the linear range was 0.006-200 nM (R2 = 0.995). This probe was reasonably free from interference, showing a good response to deoxyribonucleic acid, with strong application data from actual samples.

Electrochemiluminescent resonance energy transfer of polymer dots for aptasensing

2018 ◽  
Vol 100 ◽  
pp. 28-34 ◽  
Author(s):  
Feng Sun ◽  
Ziyu Wang ◽  
Yaqiang Feng ◽  
Yixiang Cheng ◽  
Huangxian Ju ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Polymer Dots
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