Ecstasy-induced reduction of the availability of the brain serotonin transporter as revealed by [11C](+)McN5652-PET and the multi-linear reference tissue model: loss of transporters or artifact of tracer kinetic modelling?

2006 ◽  
Vol 21 (6) ◽  
pp. 628-634 ◽  
Author(s):  
Ralph Buchert ◽  
Frank Thiele ◽  
Rainer Thomasius ◽  
Florian Wilke ◽  
Kay Petersen ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Kinetic Modelling ◽  
Brain Serotonin ◽  
Reference Tissue ◽  
Tissue Model ◽  
Tracer Kinetic Modelling ◽  
Tracer Kinetic ◽  
Reference Tissue Model ◽  
The Brain

scholarly journals Kinetic Modelling and Test–Retest Reproducibility for the Dopamine D1R Radioligand [11C]SCH23390 in Healthy and Diseased Mice

2020 ◽  
Author(s):  
Daniele Bertoglio ◽  
Jeroen Verhaeghe ◽  
Alan Miranda ◽  
Leonie Wyffels ◽  
Sigrid Stroobants ◽  
...  
Keyword(s):  
Kinetic Modelling ◽  
Regional Analysis ◽  
Accurate Estimate ◽  
Pet Scan ◽  
Binding Potential ◽  
Pharmacokinetic Modelling ◽  
Reference Tissue ◽  
Perfect Agreement ◽  
Tissue Model ◽  
Reference Tissue Model

Abstract Purpose Our aim in this study was to compare different non-invasive pharmacokinetic models and assess test–retest reproducibility of the radioligand [11C]SCH23390 for the quantification of dopamine D1-like receptor (D1R) in both wild-type (WT) mice and heterozygous (HET) Q175DN mice as Huntington’s disease (HD) model. Procedures Adult WT (n = 9) and HET (n = 14) mice underwent a 90-min [11C]SCH23390 positron emission tomography (PET) scan followed by computed tomography (CT) to evaluate the pharmacokinetic modelling in healthy and diseased conditions. Additionally, 5 WT mice and 7 HET animals received a second [11C]SCH23390 PET scan for test–retest reproducibility. Parallel assessment of the simplified reference tissue model (SRTM), the multilinear reference tissue model (MRTM) and the Logan reference tissue model (Logan Ref) using the striatum as a receptor-rich region and the cerebellum as a receptor-free (reference) region was performed to define the most suitable method for regional- and voxel-based quantification of the binding potential (BPND). Finally, standardised uptake value ratio (SUVR-1) was assessed as a potential simplified measurement. Results For all models, we measured a significant decline in dopamine D1R density (e.g. SRTM = − 38.5 ± 5.0 %, p < 0.0001) in HET mice compared to WT littermates. Shortening the 90-min scan duration resulted in large underestimation of striatal BPND in both WT mice (SRTM 60 min: − 17.7 ± 2.8 %, p = 0.0078) and diseased HET (SRTM 60 min: − 13.1 ± 4.1 %, p = 0.0001). Striatal BPND measurements were very reproducible with an average test–retest variability below 5 % when using both MRTM and SRTM. Parametric BPND maps generated with SRTM were highly reliable, showing nearly perfect agreement to the regional analysis (r2 = 0.99, p < 0.0001). Finally, SRTM provided the most accurate estimate for relative tracer delivery R1 with both regional- and voxel-based analyses. SUVR-1 at different time intervals were not sufficiently reliable when compared to BPND (r2 < 0.66). Conclusions Ninety-minute acquisition and the use of SRTM for pharmacokinetic modelling is recommended. [11C]SCH23390 PET imaging demonstrates optimal characteristics for the study of dopamine D1R density in models of psychiatric and neurological disorders as exemplified in the Q175DN mouse model of HD.

Primary Structure of the Human Platelet Serotonin Uptake Site: Identity with the Brain Serotonin Transporter

1993 ◽  
Vol 60 (6) ◽  
pp. 2319-2322 ◽  
Author(s):  
Klaus-Peter Lesch ◽  
Benjamin L. Wolozin ◽  
Dennis L. Murphy ◽  
Peter Riederer
Keyword(s):  
Serotonin Transporter ◽  
Primary Structure ◽  
Human Platelet ◽  
Serotonin Uptake ◽  
Brain Serotonin ◽  
Platelet Serotonin ◽  
The Brain

Evaluation of reference tissue model and tissue ratio method for 5-HTT using [123I] ADAM tracer

2008 ◽  
Vol 92 (3) ◽  
pp. 294-298 ◽  
Author(s):  
Bang-Hung Yang ◽  
Shyh-Jen Wang ◽  
Yuan-Hwa Chou ◽  
Tung-Ping Su ◽  
Shih-Pei Chen ◽  
...  
Keyword(s):  
Ratio Method ◽  
Reference Tissue ◽  
Tissue Model ◽  
Reference Tissue Model

An extended simplified reference tissue model for the quantification of dynamic PET with amphetamine challenge

NeuroImage ◽  
2006 ◽  
Vol 33 (2) ◽  
pp. 550-563 ◽  
Author(s):  
Yun Zhou ◽  
Ming-Kai Chen ◽  
Christopher J. Endres ◽  
Weiguo Ye ◽  
James R. Brašić ◽  
...  
Keyword(s):  
Dynamic Pet ◽  
Reference Tissue ◽  
Tissue Model ◽  
Reference Tissue Model ◽  
Simplified Reference Tissue Model
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