Evidence for a “metabolically inactive” inorganic phosphate pool in adenosine triphosphate synthase reaction using localized 31P saturation transfer magnetic resonance spectroscopy in the rat brain at 11.7 T

With the increased spectral resolution made possible at high fields, a second, smaller inorganic phosphate resonance can be resolved on 31P magnetic resonance spectra in the rat brain. Saturation transfer was used to estimate de novo adenosine triphosphate synthesis reaction rate. While the main inorganic phosphate pool is used by adenosine triphosphate synthase, the second pool is inactive for this reaction. Accounting for this new pool may not only help us understand 31P magnetic resonance spectroscopy metabolic profiles better but also better quantify adenosine triphosphate synthesis.