Immunohistochemical evaluation of immune cell infiltration in canine gliomas

Abstract Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. the study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system. Results: A total of 153 males’ prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

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Characterization of Immune Cell Infiltration Into Canine Intracranial Meningiomas

Veterinary Pathology ◽

10.1177/0300985811417249 ◽

2011 ◽

Vol 49 (5) ◽

pp. 784-795 ◽

Cited By ~ 13

Author(s):

L. B. Boozer ◽

T. W. Davis ◽

L. B. Borst ◽

K. M. Zseltvay ◽

N. J. Olby ◽

...

Keyword(s):

Immune Cell ◽

Inflammatory Cells ◽

Cell Infiltration ◽

Small Subset ◽

Immune Cell Infiltration ◽

Prognostic Information ◽

Intracranial Meningiomas ◽

Large Numbers ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded

Meningiomas are the most common intracranial tumors in dogs. A variety of inflammatory cells have been shown to invade these tumors in people, but little is known about interactions between the immune system and naturally occurring brain tumors in dogs. The purpose of this study was to investigate the presence of a variety of immune cell subsets within canine intracranial meningiomas. Twenty-three formalin-fixed, paraffin-embedded tumor samples were evaluated using immunohistochemistry with antibodies specific for CD3, CD79a, CD18, CD11d (αD), CD45RA, forkhead box P3, and Toll-like receptors 4 and 9. Immune cell infiltration was evident in all samples, with a predominance of CD3+ T cells. Large numbers of CD18+ microglia and macrophages were noted surrounding and infiltrating the tumors, and a subset of these cells within the tumor appeared to be CD11d+. Scattered macrophages at the tumor–brain interface were TLR4+ and TLR9+. Rare CD79a+ B cells were noted in only a small subset of tumors. Lesser numbers of lymphocytes that were CD11d+, CD45RA+, or FoxP3+ were noted in a number of the meningiomas. Although the function of these cells is not yet clear, work in other species suggests that evaluation of this immune cell infiltrate may provide important prognostic information and may be useful in the design of novel therapies.

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Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions

10.21203/rs.2.11132/v2 ◽

2020 ◽

Author(s):

Eiman Siddig Ahmed ◽

Lubna S. Elnour ◽

Rowa Hassan ◽

Emmanuel Edwar Siddig ◽

Mintu Elsa Chacko ◽

...

Keyword(s):

Family History ◽

Cyclin D1 ◽

P Value ◽

Low Grade ◽

High Grade ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Embedded Blocks ◽

History Of ◽

Formalin Fixed

Abstract Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. the study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system. Results: A total of 153 males’ prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

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Time and tumor type (primary or metastatic) do not influence the detection of BRAF/NRAS mutations in formalin fixed paraffin embedded samples from melanomas

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2015-1048 ◽

2016 ◽

Vol 54 (11) ◽

Cited By ~ 1

Author(s):

Miriam Potrony ◽

Celia Badenas ◽

Bénédicte Naerhuyzen ◽

Paula Aguilera ◽

Joan Anton Puig-Butille ◽

...

Keyword(s):

Melanoma Patient ◽

Primary Melanoma ◽

Sentinel Lymph Nodes ◽

Tumor Type ◽

Formalin Fixed Paraffin ◽

Ffpe Samples ◽

Formalin Fixed Paraffin Embedded ◽

Complete Sequencing ◽

Formalin Fixed ◽

Tumor Area

AbstractBackground:Methods:DNA was obtained from 144 FFPE samples (62 primary melanoma, 43 sentinel lymph nodes [SLN] and 39 metastasis).Results:Complete sequencing results were obtained from 75% (108/144) of the samples, and at least one gene was sequenced in 89% (128/144) of them.Conclusions:Preserving sufficient tumor area in FFPE blocks is important. It is necessary to keep the FFPE blocks, no matter their age, as they are necessary to decide the best treatment for the melanoma patient.

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Expression of FOXP3 in Canine Gliomas: Immunohistochemical Study of Tumor-Infiltrating Regulatory Lymphocytes

Journal of Neuropathology & Experimental Neurology ◽

10.1093/jnen/nlz120 ◽

2019 ◽

Vol 79 (2) ◽

pp. 184-193 ◽

Cited By ~ 2

Author(s):

Dolors Pi Castro ◽

Roberto José-López ◽

Francisco Fernández Flores ◽

Rosa M Rabanal Prados ◽

Maria Teresa Mandara ◽

...

Keyword(s):

Immune Cell ◽

Low Grade ◽

Similar Distribution ◽

High Grade ◽

Human Gliomas ◽

Forkhead Box ◽

Astrocytic Gliomas ◽

The Difference ◽

Regulatory Lymphocytes ◽

The Brain

Abstract Dogs develop gliomas with similar histopathological features to human gliomas and share with them the limited success of current therapeutic regimens such as surgery and radiation. The tumor microenvironment in gliomas is influenced by immune cell infiltrates. The present study aims to immunohistochemically characterize the tumor-infiltrating lymphocyte (TIL) population of naturally occurring canine gliomas, focusing on the expression of Forkhead box P3-positive (FOXP3+) regulatory T-cells (Tregs). Forty-three canine gliomas were evaluated immunohistochemically for the presence of CD3+, FOXP3+, and CD20+ TILs. In low-grade gliomas, CD3+ TILs were found exclusively within the tumor tissue. In high-grade gliomas, they were present in significantly higher numbers throughout the tumor and in the brain-tumor junction. CD20+ TILs were rarely found in comparison to CD3+ TILs. FOXP3+ TILs shared a similar distribution with CD3+ TILs. The accumulation of FOXP3+ Tregs within the tumor was more pronounced in astrocytic gliomas than in tumors of oligodendroglial lineage and the difference in expression was significant when comparing low-grade oligodendrogliomas and high-grade astrocytomas. Only high-grade astrocytomas presented FOXP3+ cells with tumoral morphology. In spontaneous canine gliomas, TILs display similar characteristics (density and distribution) as described for human gliomas, supporting the use of the dog as an animal model for translational immunotherapeutic studies.

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Tumour immune cell infiltration and survival after platinum-based chemotherapy in high-grade serous ovarian cancer subtypes: A gene expression-based computational study

EBioMedicine ◽

10.1016/j.ebiom.2019.102602 ◽

2020 ◽

Vol 51 ◽

pp. 102602 ◽

Cited By ~ 10

Author(s):

Rong Liu ◽

Rong Hu ◽

Ying Zeng ◽

Wei Zhang ◽

Hong-Hao Zhou

Keyword(s):

Gene Expression ◽

Ovarian Cancer ◽

Immune Cell ◽

Computational Study ◽

Cell Infiltration ◽

Immune Cell Infiltration ◽

High Grade ◽

Serous Ovarian Cancer ◽

Cancer Subtypes ◽

Platinum Based Chemotherapy

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Deciphering the Immune Microenvironment on A Single Archival Formalin-Fixed Paraffin-Embedded Tissue Section by An Immediately Implementable Multiplex Fluorescence Immunostaining Protocol

Cancers ◽

10.3390/cancers12092449 ◽

2020 ◽

Vol 12 (9) ◽

pp. 2449 ◽

Cited By ~ 2

Author(s):

Adrien Guillot ◽

Marlene S. Kohlhepp ◽

Alix Bruneau ◽

Felix Heymann ◽

Frank Tacke

Keyword(s):

Immune Cell ◽

Simple Procedure ◽

Parenchymal Cell ◽

Ffpe Tissue ◽

Immune Microenvironment ◽

Tissue Samples ◽

Tissue Organization ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

Technological breakthroughs have fundamentally changed our understanding on the complexity of the tumor microenvironment at the single-cell level. Characterizing the immune cell composition in relation to spatial distribution and histological changes may provide important diagnostic and therapeutic information. Immunostaining on formalin-fixed paraffin-embedded (FFPE) tissue samples represents a widespread and simple procedure, allowing the visualization of cellular distribution and processes, on preserved tissue structure. Recent advances in microscopy and molecular biology have made multiplexing accessible, yet technically challenging. We herein describe a novel, simple and cost-effective method for a reproducible and highly flexible multiplex immunostaining on archived FFPE tissue samples, which we optimized for solid organs (e.g., liver, intestine, lung, kidney) from mice and humans. Our protocol requires limited specific equipment and reagents, making multiplexing (>12 antibodies) immediately implementable to any histology laboratory routinely performing immunostaining. Using this method on single sections and combining it with automated whole-slide image analysis, we characterize the hepatic immune microenvironment in preclinical mouse models of liver fibrosis, steatohepatitis and hepatocellular carcinoma (HCC) and on human-patient samples with chronic liver diseases. The data provide useful insights into tissue organization and immune–parenchymal cell-to-cell interactions. It also highlights the profound macrophage heterogeneity in liver across premalignant conditions and HCC.

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Programmed death-ligand 1 (PD-L1) expression in cured and not cured testicular and other germ cell tumors (GCT).

Journal of Clinical Oncology ◽

10.1200/jco.2016.34.2_suppl.485 ◽

2016 ◽

Vol 34 (2_suppl) ◽

pp. 485-485 ◽

Cited By ~ 1

Author(s):

Brandon David Bernard ◽

Laurence Albiges ◽

Sabina Signoretti ◽

Jesse Novak ◽

Michelle S. Hirsch ◽

...

Keyword(s):

Immune Cell ◽

Therapeutic Option ◽

Germ Cell Tumors ◽

Unknown Origin ◽

Mediastinal Tumors ◽

Formalin Fixed Paraffin ◽

Programmed Death ◽

Formalin Fixed Paraffin Embedded ◽

Tumor Types ◽

Formalin Fixed

485 Background: PD-L1 is involved in immune regulation and is prognostic in many tumor types. The aim of this study was to assess PD-L1 expression in orchiectomy and metastases (mets) from GCT and to assess for an association with outcome. Methods: Immunohistochemistry (IHC) on whole sections from archival formalin-fixed paraffin-embedded tissue from Dana-Farber Cancer Institute (DFCI) and Gustave Roussy (IGR) was performed using anti-PD-L1 antibody E1L3N. Stained slides were scored semi-quantitatively and assessed independently. At DFCI, PD-L1 was scored as percent of positive tumor cells (TC) and extent of positive immune cell (IC) infiltrate; at IGR, a cut-off of 5% defined positive TC and IC. PD-L1 status was assessed by site, histology and chemotherapy (CT) status. PD-L1 status was correlated with clinical outcome. Most samples at DFCI were pre-CT (74.2%) whereas most at IGR were post-CT (85.4%). Results: IHC from 171 patients with GCT (89 DFCI, 82 IGR) from 1987-2014 was reviewed. The DFCI cohort included 69 orchiectomy, 15 mets and 5 unknown origin with 28 total deaths; the IGR cohort included 26 lung mets, 36 mediastinal or retroperitoneal lymph node mets and 20 primary mediastinal tumors. DFCI had 38 seminoma (S), 50 nonseminoma (NS) and 1 unknown; at IGR, 13 S and 69 NS. In both cohorts, PD-L1 staining in IC was higher in S (DFCI 33/38 moderate (mod)-high (86.8%); IGR 12/13 positive (92.3%)) vs. NS (DFCI 26/50 mod-high (52.0%); IGR 35/68 positive (51.5%)) (DFCI p = 0.003; IGR p = 0.006). At DFCI, 5 samples had PD-L1 positive TC (2 mixed GCT, 2 pure choriocarcinomas (CC) and 1 S); at IGR, 14 had positive TC (all NS, including 11 pure CC). More PD-L1 positive TC were CC than other GCT types (DFCI p = 0.003; IGR p < 0.001). In 36 S orchiectomy samples at DFCI, 0/4 that scored none-mild died and 2/31 (6.5%) that scored mod-high died (p = 1.00); in 30 NS orchiectomy samples, 4/9 (44.4%) that scored none-mild died and 2/16 (12.5%) that scored mod-high died (p = 0.14). Conclusions: Higher PD-L1 status in orchiectomy is not associated with GCT survival. PD-L1 positive TC may serve as a histological marker for CC among NS. Higher PD-L1 expression on IC in S and on TC in CC may point to immunotherapy as a therapeutic option in these tumors.

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