scholarly journals Salivary Melatonin as a Circadian Phase Marker: Validation and Comparison to Plasma Melatonin

1997 ◽  
Vol 12 (5) ◽  
pp. 457-466 ◽  
Author(s):  
Athena Voultsios ◽  
David J. Kennaway ◽  
Drew Dawson
Keyword(s):  
Circadian Phase ◽  
Marker Validation ◽  
Plasma Melatonin ◽  
Salivary Melatonin

Relationship between plasma and salivary melatonin and cortisol investigated by LC-MS/MS

2017 ◽  
Vol 55 (9) ◽  
Author(s):  
Martijn van Faassen ◽  
Rainer Bischoff ◽  
Ido P. Kema
Keyword(s):  
Mass Spectrometry ◽  
Equilibrium Dialysis ◽  
Added Value ◽  
The Novel ◽  
Plasma Melatonin ◽  
Disease States ◽  
Validation Criteria ◽  
Significant Difference ◽  
Salivary Melatonin ◽  
Free Plasma

AbstractBackground:Disturbance of the circadian rhythm has been associated with disease states, such as metabolic disorders, depression and cancer. Quantification of the circadian markers such as melatonin and cortisol critically depend on reliable and reproducible analytical methods. Previously, melatonin and cortisol were primarily analyzed separately, mainly using immunoassays.Methods:Here we describe the validation and application of a high-throughput liquid chromatography in combination with mass spectrometry (LC-MS/MS) method for the combined analysis of melatonin and cortisol in plasma and saliva. The LC-MS/MS method was validated according to international validation guidelines. We used this method to analyze total plasma, free plasma (as obtained by equilibrium dialysis) and saliva melatonin and cortisol in healthy adults.Results:Validation results for plasma and saliva melatonin and cortisol were well within the international validation criteria. We observed no difference between saliva collected by passive drooling or Salivette. Moreover, we noted a significant difference in saliva vs. free plasma melatonin. We observed on average 36% (95% CI: 4%–60%) higher salivary melatonin levels in comparison to free plasma melatonin, suggestive of local production of melatonin in the salivary glands.Conclusions:The novel outcome of this study is probably due to the high precision of our LC-MS/MS assay. These outcomes illustrate the added value of accurate and sensitive mass spectrometry based methods for the quantification of neuroendocrine biomarkers.

scholarly journals 0994 Assessment Of Sleep-wake And Circadian Rhythm Disruption In Children And Adolescents Diagnosed With Craniopharyngioma

SLEEP ◽  
2020 ◽  
Vol 43 (Supplement_1) ◽  
pp. A377-A378
Author(s):  
K N LaRosa ◽  
S J Crowley ◽  
D Hancock ◽  
M Caples ◽  
T E Merchant ◽  
...  
Keyword(s):  
Circadian Rhythm ◽  
Cancer Center ◽  
Circadian Phase ◽  
Excessive Sleepiness ◽  
Increased Risk ◽  
Sampling Window ◽  
Salivary Melatonin ◽  
Sleep Diagnosis ◽  
Multiple Sleep Latency ◽  
Simultaneous Assessment

Abstract Introduction Patients with craniopharyngioma are at increased risk for hypersomnia/narcolepsy and circadian rhythm disruption, secondary to hypothalamic-pituitary involvement of the tumor. We assessed youth with craniopharyngioma to determine presence of the dim light melatonin onset (DLMO) and concurrent sleep disturbance. Methods Fifty-two patients (7-21 years; 51% female) enrolled on our institutional protocol for craniopharyngioma that included surgery, proton therapy, or both. In-home salivary melatonin was collected after surgery and hourly beginning 3 h before to 1 h after habitual bedtime to determine the DLMO, which was defined as the time that melatonin exceeded a 4 pg/mL threshold. Polysomnography and a next day multiple sleep latency test (MSLT) were also conducted. Results Hypersomnia/narcolepsy was indicated in 86% of patients. DLMO was detected in 29 (56%) patients and averaged 21:04 (±1:14). All but 2 patients with a DLMO had a concurrent sleep diagnosis (18 hypersomnia, 8 narcolepsy, 1 insomnia). In those we could not compute a DLMO, melatonin was above the 4 pg/mL threshold in 19 (37%), suggesting that the DLMO was likely earlier than the sampling window. Two (4%) did not reach threshold, suggesting that the DLMO was later than the window. For patients in which DLMO was not computed, all but 4 had a concurrent sleep diagnosis (7 hypersomnia, 9 narcolepsy, 1 MSLT not completed). Three (6%) participants showed a pattern of melatonin decreasing before bedtime (2 hypersomnia, 1 narcolepsy). Sleep disorder diagnosis was not associated with whether a DLMO was detected or not. Conclusion DLMO did not occur within the sampling window in 44% of patients with the majority due to the DLMO likely occurring before sampling started. Simultaneous assessment of both sleep-wake disturbance and circadian phase could provide more informed sleep interventions for excessive sleepiness and circadian misalignment in this patient population. Support This study was supported by cancer center grant (CA21765) from the National Cancer Institute, and ALSAC.

Morning bright light therapy: a helpful tool for reducing comorbid symptoms of affective and behavioral dysregulation in juvenile depressed inpatients? A pilot trial

2017 ◽  
Vol 45 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Sarah Bogen ◽  
Tanja Legenbauer ◽  
Stephanie Gest ◽  
Martin Holtmann
Keyword(s):  
Pilot Trial ◽  
Secondary Analysis ◽  
Bright Light ◽  
Light Therapy ◽  
Bright Light Therapy ◽  
Circadian Phase ◽  
Behavioral Dysregulation ◽  
Sleep Parameters ◽  
Circadian Effect ◽  
Dysregulation Profile

Abstract. Objective: In recent years, bright light therapy (BLT) has been used to treat depression and to stabilize circadian rhythms. In this study we evaluated whether it is also helpful for comorbid symptoms of affective and behavioral dysregulation in depressive inpatients. Method: This article reports a secondary analysis comparing two subgroups of depressive participants with comorbid affective and behavioral dysregulation, captured with the dysregulation-profile of the Strengths and Difficulties Questionnaire (SDQ-DP; n = 16 vs. n = 11). Participants were randomly allocated to active BLT (10,000 lux) or control BLT (approx. 100 lux), and received 45 minutes of BLT for 2 weeks. SDQ-DP scores, sleep parameters, and circadian preference were assessed at baseline, after the intervention, and 3 weeks later. Results: No direct effects on SDQ-DP scores were observed. Sleep improved in both conditions. Only in the active BLT condition was a circadian phase advance found. Correlation and regression analyses indicated an indirect, circadian effect for improved SDQ-DP scores. Conclusions: The data of this pilot trial should be considered preliminary and merely descriptive. Further research is warranted.


Pulsatile GnRH administration is attended by decreased plasma melatonin in patients suffering from GnRH deficiency

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S50-S51
Author(s):  
R. P. WILLIG ◽  
J. C. COMMENTZ ◽  
N. STAHNKE
Keyword(s):  
Plasma Melatonin ◽  
Pulsatile Gnrh

The Effect of Moderate Weight Loss on Overnight Melatonin Secretion

1990 ◽  
Vol 156 (6) ◽  
pp. 875-877 ◽  
Author(s):  
I. M. Anderson ◽  
S. E. Gartside ◽  
P. J. Cowen
Keyword(s):  
Weight Loss ◽  
Melatonin Level ◽  
Melatonin Secretion ◽  
Plasma Melatonin ◽  
Healthy Women ◽  
Before And After

Overnight plasma melatonin level was measured in ten healthy women before and after a 4300 kJ (1000 kcaI) diet in which they lost an average 3.1 kg. This weight loss did not significantly alter melatonin levels.

scholarly journals Comment Concerning the Effects of Light Intensity on Melatonin Suppression in the Review “Light Modulation of Human Clocks, Wake, and Sleep” by A. Prayag et al.

2021 ◽  
Vol 3 (1) ◽  
pp. 181-188
Author(s):  
Peter Bracke ◽  
Eowyn Van de Putte ◽  
Wouter R. Ryckaert
Keyword(s):  
Phase Shift ◽  
Light Intensity ◽  
Dose Response ◽  
Light Modulation ◽  
Response Curves ◽  
Practical Applications ◽  
Circadian Phase ◽  
Melatonin Suppression ◽  
Dose Response Curves ◽  
Circadian Phase Shift

Dose-response curves for circadian phase shift and melatonin suppression in relation to white or monochromatic nighttime illumination can be scaled to melanopic weighed illumination for normally constricted pupils, which makes them easier to interpret and compare. This is helpful for a practical applications.

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