Attenuation of hydrogenperoxide-induced oxidative damages in L929 fibroblast cells by sulfated polysaccharides isolated from the edible marine algae Padina tetrastromatica

2019 ◽  
Vol 34 (2) ◽  
pp. 150-162 ◽  
Author(s):  
Geena Mariya Jose ◽  
Mahadevan Raghavankutty ◽  
G Muraleedhara Kurup
Keyword(s):  
Reactive Oxygen Species ◽  
Marine Algae ◽  
Reactive Oxygen ◽  
Sulfated Polysaccharides ◽  
Fibroblast Cells ◽  
Oxygen Species ◽  
Glutathione System ◽  
L929 Cells ◽  
Padina Tetrastromatica ◽  
L929 Fibroblast

Sulfated polysaccharides are polymers with potent biological roles such as antioxidant, anticancer, anti-inflammatory, and immunomodulatory activities. They are gaining significance in advanced healthcare research, since they can control oxidative cellular damages by arresting the reactive oxygen species. Hence, this study aims to evaluate the cellular antioxidant potential of sulfated polysaccharides isolated from the marine algae Padina tetrastromatica in L929 cells. The monosaccharide composition of sulfated polysaccharides was determined using high-performance liquid chromatography. The protective effect of sulfated polysaccharides on L929 fibroblast cells under H2O2-induced oxidative stress has been evaluated using MTT assay, acridine orange/ethidium bromide staining, flow cytometry, comet assay, DCFH-DA staining, fluorometry, and biochemical assays. Sulfated polysaccharides were found to contain glucose, fucose, xylose, and uronic acid. It was non-toxic to L929 cells and effectively increased the viability of mouse fibroblast cells under induced oxidative stress. Various cellular damages such as apoptosis, plasma membrane alterations, DNA damage, and lipid peroxidation induced by H2O2 have been attenuated by sulfated polysaccharides. This might be due to the reduction of intracellular reactive oxygen species level by sulfated polysaccharides as observed in DCFH-DA staining. Sulfated polysaccharides improved the activity of intracellular antioxidant status and glutathione system. The augmentation of endogenous antioxidant enzymes and glutathione system by sulfated polysaccharides can be the possible reason for the reduction of intracellular reactive oxygen species, prevention of apoptosis, and betterment of viability in L929 cells. The study confirms the potent antioxidant activity of the sulfated polysaccharides in addition to their structural and nutritional roles.

scholarly journals Reactive oxygen species induce Cox-2 expression via TAK1 activation in synovial fibroblast cells

FEBS Open Bio ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 492-501 ◽  
Author(s):  
Yuta Onodera ◽  
Takeshi Teramura ◽  
Toshiyuki Takehara ◽  
Kanae Shigi ◽  
Kanji Fukuda
Keyword(s):  
Reactive Oxygen Species ◽  
Synovial Fibroblast ◽  
Reactive Oxygen ◽  
Fibroblast Cells ◽  
Oxygen Species ◽  
Cox 2

scholarly journals Reactive Oxygen Species Drive Epigenetic Changes in Radiation-Induced Fibrosis

2019 ◽  
Vol 2019 ◽  
pp. 1-27 ◽  
Author(s):  
Shashank Shrishrimal ◽  
Elizabeth A. Kosmacek ◽  
Rebecca E. Oberley-Deegan
Keyword(s):  
Reactive Oxygen Species ◽  
Extracellular Matrix ◽  
Radiation Exposure ◽  
Reactive Oxygen ◽  
Matrix Proteins ◽  
Antioxidant Compounds ◽  
Fibroblast Cells ◽  
Oxygen Species ◽  
Epigenetic Changes ◽  
Radiation Induced

Radiation-induced fibrosis (RIF) develops months to years after initial radiation exposure. RIF occurs when normal fibroblasts differentiate into myofibroblasts and lay down aberrant amounts of extracellular matrix proteins. One of the main drivers for developing RIF is reactive oxygen species (ROS) generated immediately after radiation exposure. Generation of ROS is known to induce epigenetic changes and cause differentiation of fibroblasts to myofibroblasts. Several antioxidant compounds have been shown to prevent radiation-induced epigenetic changes and the development of RIF. Therefore, reviewing the ROS-linked epigenetic changes in irradiated fibroblast cells is essential to understand the development and prevention of RIF.

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