Technical Considerations for Developing Enzyme Immunohistochemical Staining Procedures on Formalin-Fixed Paraffin-Embedded Tissues for Diagnostic Pathology

This study examined immunohistochemical staining patterns for several meningioma variants involving either the brain or spinal cord of dogs. Formalin-fixed, paraffin-embedded tissue from 15 tumors was obtained. The selected tumor group included seven meningothelial, three transitional, two malignant (anaplastic), one myxoid, one papillary, and one osteomatous meningiomas. Tumors were evaluated for reactivity to the following six immunohistochemical markers: vimentin, pancytokeratin, glial fibrillary acidic protein (GFAP), S100, neuron-specific enolase (NSE), and synaptophysin. Vimentin expression was detected in all meningiomas, and 14 of 15 tumors demonstrated intense vimentin staining in more than 50% of the neoplastic cells. Pancytokeratin expression was present in 11 of 15 neoplasms; however, positive staining frequently was focal and often involved a small percentage of the neoplastic cells. GFAP expression was detected in a single, anaplastic meningioma. Although expression of NSE and S100 was detected in 12 of 25 meningiomas, the intensity of the staining and the percentage of positive neoplastic cells was highly variable. Synaptophysin was uniformly negative. These results will help to establish immunohistochemical profiles for meningiomas that will improve our ability to correctly differentiate these neoplasms of meningeal origin from central nervous system tumors originating from other sites.

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Antibody validation and scoring guidelines for ABCG2 immunohistochemical staining in formalin-fixed paraffin-embedded colon cancer tissue

Scientific Reports ◽

10.1038/srep26997 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 3

Author(s):

Camilla Natasha Cederbye ◽

Jesper Andreas Palshof ◽

Tine Plato Hansen ◽

Anne Katrine Duun-Henriksen ◽

Dorte Linnemann ◽

...

Keyword(s):

Colon Cancer ◽

Immunohistochemical Staining ◽

Cancer Tissue ◽

Colon Cancer Tissue ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Antibody Validation ◽

Formalin Fixed

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The use of combined PCR, fluorescence in situ hybridization and immunohistochemical staining to diagnose mucormycosis from formalin‐fixed paraffin‐embedded tissues

Mycoses ◽

10.1111/myc.13382 ◽

2021 ◽

Author(s):

Xiao Liu ◽

Yinggai Song ◽

Ruoyu Li

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Immunohistochemical Staining ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Immunohistochemical staining of dystrophin on formalin-fixed paraffin-embedded sections in Duchenne/Becker muscular dystrophy and manifesting carriers of Duchenne muscular dystrophy

Neuromuscular Disorders ◽

10.1016/s0960-8966(99)00116-9 ◽

2000 ◽

Vol 10 (6) ◽

pp. 425-429 ◽

Cited By ~ 8

Author(s):

Sachiko Hoshino ◽

Norio Ohkoshi ◽

Masahiko Watanabe ◽

Shin'ichi Shoji

Keyword(s):

Duchenne Muscular Dystrophy ◽

Muscular Dystrophy ◽

Immunohistochemical Staining ◽

Becker Muscular Dystrophy ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Tumorigenic Factor CRIPTO-1 Is Immunolocalized in Extravillous Cytotrophoblast in Placenta Creta

BioMed Research International ◽

10.1155/2014/892856 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Carla Letícia Bandeira ◽

Alexandre Urban Borbely ◽

Rossana Pulcineli Vieira Francisco ◽

Regina Schultz ◽

Marcelo Zugaib ◽

...

Keyword(s):

Immunohistochemical Staining ◽

Extravillous Trophoblast ◽

Cell Type ◽

Third Trimester ◽

Morphological Examination ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Extravillous Cytotrophoblast ◽

Formalin Fixed ◽

Migratory Phenotype

CRIPTO-(CR)1 is a protein associated with tumorigenesis and metastasis. Here we demonstrate that CR-1 expression in normal and creta placentas is associated with various degrees of uterine invasion. Cytokeratin (CK) and CR-1 protein expression was visualized by immunohistochemical staining of formalin-fixed, paraffin-embedded placental specimens (control placentas,n=9; accreta,n=6; increta,n=10; percreta,n=15). The pattern of extravillous trophoblast (EVT) cell morphology was distinctive in creta placentas: densely-compacted cell columns and large star-shaped cells with a typically migratory phenotype, not common among third trimester control placentas. Quantification revealed higher CR-1 immunoreactivities in accreta(P=0.001), increta(P=0.0002), and percreta placentas(P=0.001)than in controls. In contrast to controls, there was a significant positive relationship between CR-1 and CK reactivity in all creta placentas (accreta,P=0.02; increta,P=0.0001, and percreta,P=0.025). This study demonstrated CR-1 expression in the placental bed, its increased expression in creta placentas, and EVT cells as the main CR-1-producing cell type. Morphological examination revealed an immature and invasive trophoblast profile in creta placentas, suggesting impairment of the trophoblast differentiation pathway. These findings provide important new insights into the pathophysiology of abnormal creta placentation and its gestational consequences.

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The effect of prolonged decalcification on the immunohistochemical staining of Leishmania infantum amastigotes in formalin-fixed, paraffin-embedded canine soft tissues (lymph nodes)

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.14951 ◽

2018 ◽

Vol 59 (1) ◽

pp. 77

Author(s):

E PAPADOGIANNAKIS ◽

A KOUTINAS ◽

V KONTOS

Keyword(s):

Lymph Node ◽

Leishmania Infantum ◽

Immunohistochemical Staining ◽

Soft Tissues ◽

Immunohistochemical Detection ◽

Cell Cytoplasm ◽

Host Cell Cytoplasm ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

The aim of this report was to present the preliminary results of the effect of prolonged decalcification on the immunohistochemical staining of Leishmania infantum in formalin-fixed, paraffin-embedded canine soft tissues. A heavily parasitized by Leishmania infantum amastigotes popliteal lymph node was used in this study. One half of a surgically excised lymphnode was processed according to a decalcification protocol (for 40 days), while the other half was processed without decalcification. Immunohistochemical staining for Leishmania infantum was then applied on both decalcified and non-decalcified lymph node sections. The amastigotes were stained and detected in non-decalcified sections, whereas a significant reduction of immunostainingwas observed in decalcified sections. It is likely that parasite antigens are altered by the decalcification procedure, probably as a result of the long lasting effect of decalcifying acids on both the amastigotes and the host cell cytoplasm. Consequently, it seems that immunohistochemical detection of Leishmania infantum amastigotes is significantly affected following prolonged tissue decalcification.

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