scholarly journals Immunohistochemical Staining Patterns of Canine Meningiomas and Correlation with Published Immunophenotypes

2002 ◽  
Vol 39 (3) ◽  
pp. 311-321 ◽  
Author(s):  
K. F. Barnhart ◽  
J. Wojcieszyn ◽  
R. W. Storts
Keyword(s):  
Immunohistochemical Staining ◽  
Neuron Specific Enolase ◽  
Positive Staining ◽  
Neoplastic Cells ◽  
Vimentin Expression ◽  
Immunohistochemical Markers ◽  
Formalin Fixed Paraffin ◽  
Tumor Group ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

This study examined immunohistochemical staining patterns for several meningioma variants involving either the brain or spinal cord of dogs. Formalin-fixed, paraffin-embedded tissue from 15 tumors was obtained. The selected tumor group included seven meningothelial, three transitional, two malignant (anaplastic), one myxoid, one papillary, and one osteomatous meningiomas. Tumors were evaluated for reactivity to the following six immunohistochemical markers: vimentin, pancytokeratin, glial fibrillary acidic protein (GFAP), S100, neuron-specific enolase (NSE), and synaptophysin. Vimentin expression was detected in all meningiomas, and 14 of 15 tumors demonstrated intense vimentin staining in more than 50% of the neoplastic cells. Pancytokeratin expression was present in 11 of 15 neoplasms; however, positive staining frequently was focal and often involved a small percentage of the neoplastic cells. GFAP expression was detected in a single, anaplastic meningioma. Although expression of NSE and S100 was detected in 12 of 25 meningiomas, the intensity of the staining and the percentage of positive neoplastic cells was highly variable. Synaptophysin was uniformly negative. These results will help to establish immunohistochemical profiles for meningiomas that will improve our ability to correctly differentiate these neoplasms of meningeal origin from central nervous system tumors originating from other sites.

Immunohistochemical Demonstration of Desmin in Canine Smooth Muscle Tumors

1987 ◽  
Vol 24 (3) ◽  
pp. 211-215 ◽  
Author(s):  
C. B. Andreasen ◽  
E. A. Mahaffey
Keyword(s):  
Smooth Muscle ◽  
Positive Staining ◽  
Negative Results ◽  
Formalin Fixed Paraffin ◽  
Smooth Muscle Tumors ◽  
Formalin Fixed Paraffin Embedded ◽  
Immunohistochemical Methods ◽  
Formalin Fixed ◽  
Immunohistochemical Identification ◽  
Avidin Biotin Complex

Sections of formalin-fixed, paraffin-embedded canine leiomyomas, leiomyosarcomas, or fibrosarcomas were examined by immunohistochemical methods for the presence of desmin. Twenty-two leiomyomas and leiomyosarcomas were stained using the avidin-biotin complex technique, and 14 samples demonstrated positive staining for desmin. The eight negative results obtained may reflect differences in fixation or the affinity of the primary antibody for the tissues examined. Desmin was specific for myogenic tissues. Five canine fibrosarcomas examined immunohistochemically were all negative for desmin staining. The results indicate that desmin is a useful marker for immunohistochemical identification of canine leiomyomas and leiomyosarcomas.

A103 Immunostaining in the Diagnosis of Adrenal Cortical Tumors

2002 ◽  
Vol 126 (2) ◽  
pp. 170-172 ◽  
Author(s):  
Timothy S. Loy ◽  
Roy W. Phillips ◽  
Chadwick L. Linder
Keyword(s):  
Differential Diagnosis ◽  
Metastatic Carcinoma ◽  
Serous Carcinoma ◽  
Positive Staining ◽  
Adrenal Metastases ◽  
Cortical Cells ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Adrenal Cortical Cells ◽  
Formalin Fixed

Abstract Context.—The monoclonal antibody A103 recognizes an antigen on melanoma cells known as Melan-A or MART-1. Recent studies have shown that A103 also reacts with adrenal cortical cells and may be useful in the diagnosis of adrenal cortical tumors. However, only small numbers of some of the tumors in the differential diagnosis of adrenal cortical neoplasms have been studied. Objective.—To study the specificity of A103 immunohistochemistry in a large number of tumors in the differential diagnosis of adrenal cortical neoplasms. Design.—Formalin-fixed, paraffin-embedded tissue from 21 adrenal cortical tumors, 16 cases of metastatic carcinoma to the adrenal, 10 pheochromocytomas, and 269 extra-adrenal carcinomas was evaluated for A103 immunoreactivity using a commercially available antibody (Novocastra, Newcastle, UK). Results.—Positive staining was seen in all of the adrenal cortical tumors but in none of the adrenal metastases or pheochromocytomas. In the 269 extra-adrenal carcinomas, A103 immunoreactivity was limited to a single ovarian serous carcinoma. Conclusion.—A103 immunostaining is useful in distinguishing adrenal cortical neoplasms from other carcinomas and pheochromocytoma.

Primary Primitive Peripheral Neuroectodermal Tumor of the Prostate

2003 ◽  
Vol 127 (4) ◽  
pp. e190-e193 ◽  
Author(s):  
Maurizio Colecchia ◽  
Gian Paolo Dagrada ◽  
Pietro Luigi Poliani ◽  
Antonella Messina ◽  
Silvana Pilotti
Keyword(s):  
Primitive Neuroectodermal Tumor ◽  
Prostate Gland ◽  
Neuron Specific Enolase ◽  
Neuroectodermal Tumor ◽  
Peripheral Primitive Neuroectodermal Tumor ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Primitive Peripheral Neuroectodermal Tumor ◽  
Formalin Fixed

Abstract A case of primitive peripheral neuroectodermal tumor arising in the prostate gland of a 31-year-old man and first diagnosed through a biopsy is reported. Microscopically, the tumor was made up of solid nests and sheets of small round cells, and it was difficult to distinguish the neoplasm from other small round cell tumors, such as small cell carcinoma, rhabdomyosarcoma, or malignant lymphoma. Immunohistochemically, the tumor cells showed immunoreactivity for CD99, vimentin, neuron-specific enolase, and synaptophysin. The neoplasm was excised by a radical surgical procedure preceded by chemotherapy and radiation therapy. The morphologic diagnosis of the prostatectomy specimen was complemented by molecular analysis performed on viable microdissected tissue obtained from formalin-fixed, paraffin-embedded tumor sections. Polymerase chain reaction and sequencing assessment showed the presence of EWS/FLI1 type 2 chimeric transcript, confirming the diagnosis of peripheral primitive neuroectodermal tumor. To our knowledge, this is the first description of a primary peripheral primitive neuroectodermal tumor in the prostate gland.

scholarly journals Antibody validation and scoring guidelines for ABCG2 immunohistochemical staining in formalin-fixed paraffin-embedded colon cancer tissue

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Camilla Natasha Cederbye ◽  
Jesper Andreas Palshof ◽  
Tine Plato Hansen ◽  
Anne Katrine Duun-Henriksen ◽  
Dorte Linnemann ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Immunohistochemical Staining ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Antibody Validation ◽  
Formalin Fixed
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