scholarly journals Neurological and Neuropsychological Changes Associated with SARS-CoV-2 Infection: New Observations, New Mechanisms

2021 ◽  
pp. 107385842098410
Author(s):  
Muhammad Ali Haidar ◽  
Hussam Jourdi ◽  
Zeinab Haj Hassan ◽  
Ohanes Ashekyan ◽  
Manal Fardoun ◽  
...  
Keyword(s):  
Viral Entry ◽  
Health Workers ◽  
The Elderly ◽  
Circumventricular Organs ◽  
Depression And Anxiety ◽  
Virus Surface ◽  
Angiotensin Converting Enzyme 2 ◽  
Neuropsychological Changes ◽  
High Risk Populations ◽  
The Central Nervous System

SARS-CoV-2 infects cells through angiotensin-converting enzyme 2 (ACE2), a ubiquitous receptor that interacts with the virus’ surface S glycoprotein. Recent reports show that the virus affects the central nervous system (CNS) with symptoms and complications that include dizziness, altered consciousness, encephalitis, and even stroke. These can immerge as indirect immune effects due to increased cytokine production or via direct viral entry into brain tissue. The latter is possible through neuronal access via the olfactory bulb, hematogenous access through immune cells or directly across the blood-brain barrier (BBB), and through the brain’s circumventricular organs characterized by their extensive and highly permeable capillaries. Last, the COVID-19 pandemic increases stress, depression, and anxiety within infected individuals, those in isolation, and high-risk populations like children, the elderly, and health workers. This review surveys the recent updates of CNS manifestations post SARS-CoV-2 infection along with possible mechanisms that lead to them.

scholarly journals Human Immunodeficiency Viruses Pseudotyped with SARS-CoV-2 Spike Proteins Infect a Broad Spectrum of Human Cell Lines through Multiple Entry Mechanisms

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 953
Author(s):  
Chuan Xu ◽  
Annie Wang ◽  
Ke Geng ◽  
William Honnen ◽  
Xuening Wang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Viral Entry ◽  
Broad Spectrum ◽  
A549 Cells ◽  
Cell Types ◽  
Pseudotyped Virus ◽  
Angiotensin Converting Enzyme 2 ◽  
Human Immunodeficiency Viruses ◽  
Tyrosine Protein Kinase ◽  
Overexpressing Cell

Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2), the causative agent of coronavirus disease 19 (COVID-19), enters cells through attachment to the human angiotensin converting enzyme 2 (hACE2) via the receptor-binding domain (RBD) in the surface/spike (S) protein. Several pseudotyped viruses expressing SARS-CoV-2 S proteins are available, but many of these can only infect hACE2-overexpressing cell lines. Here, we report the use of a simple, two-plasmid, pseudotyped virus system comprising a SARS-CoV-2 spike-expressing plasmid and an HIV vector with or without vpr to investigate the SARS-CoV-2 entry event in various cell lines. When an HIV vector without vpr was used, pseudotyped SARS-CoV-2 viruses produced in the presence of fetal bovine serum (FBS) were able to infect only engineered hACE2-overexpressing cell lines, whereas viruses produced under serum-free conditions were able to infect a broader range of cells, including cells without hACE2 overexpression. When an HIV vector containing vpr was used, pseudotyped viruses were able to infect a broad spectrum of cell types regardless of whether viruses were produced in the presence or absence of FBS. Infection sensitivities of various cell types did not correlate with mRNA abundance of hACE2, TMPRSS2, or TMPRSS4. Pseudotyped SARS-CoV-2 viruses and replication-competent SARS-CoV-2 virus were equally sensitive to neutralization by an anti-spike RBD antibody in cells with high abundance of hACE2. However, the anti-spike RBD antibody did not block pseudotyped viral entry into cell lines with low abundance of hACE2. We further found that CD147 was involved in viral entry in A549 cells with low abundance of hACE2. Thus, our assay is useful for drug and antibody screening as well as for investigating cellular receptors, including hACE2, CD147, and tyrosine-protein kinase receptor UFO (AXL), for the SARS-CoV-2 entry event in various cell lines.

scholarly journals Effect of Coronavirus Disease 2019 in Pulmonary Circulation. The Particular Scenario of Precapillary Pulmonary Hypertension

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 548 ◽  
Author(s):  
Jorge Nuche ◽  
Teresa Segura de la Cal ◽  
Carmen Jiménez López Guarch ◽  
Francisco López-Medrano ◽  
Carmen Pérez-Olivares Delgado ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Respiratory Infections ◽  
Chronic Thromboembolic Pulmonary Hypertension ◽  
The Elderly ◽  
Angiotensin Converting Enzyme 2 ◽  
Common Features ◽  
Pulmonary Vasodilators ◽  
Thromboembolic Pulmonary Hypertension ◽  
Pulmonary Arterial ◽  
Low Incidence

The Coronavirus Disease of 2019 (COVID-19) has supposed a global health emergency affecting millions of people, with particular severity in the elderly and patients with previous comorbidities, especially those with cardiovascular disease. Patients with pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH) could represent an especially vulnerable population because of the high mortality rates reported for respiratory infections. However, the number of COVID-19 cases reported among PAH and CTEPH patients is surprisingly low. Furthermore, the clinical picture that has been described in these patients is far from the severity that experts would expect. Endothelial dysfunction is a common feature between patients with PAH/CTEPH and COVID-19, leading to ventilation/perfusion mismatch, vasoconstriction, thrombosis and inflammation. In this picture, the angiotensin-converting enzyme 2 plays an essential role, being directly involved in the pathophysiology of both clinical entities. Some of these common characteristics could explain the good adaptation of PAH and CTEPH patients to COVID-19, who could also have obtained a benefit from the disease’s specific treatments (anticoagulant and pulmonary vasodilators), probably due to its protective effect on the endothelium. Additionally, these common features could also lead to PAH/CTEPH as a potential sequelae of COVID-19. Throughout this comprehensive review, we describe the similarities and differences between both conditions and the possible pathophysiological and therapeutic-based mechanisms leading to the low incidence and severity of COVID-19 reported in PAH/CTEPH patients to date. Nevertheless, international registries should look carefully into this population for better understanding and management.

scholarly journals The Effects of Aβ1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2

2021 ◽  
Vol 22 (15) ◽  
pp. 8226
Author(s):  
John Tsu-An Hsu ◽  
Chih-Feng Tien ◽  
Guann-Yi Yu ◽  
Santai Shen ◽  
Yi-Hsuan Lee ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Viral Entry ◽  
Negative Impact ◽  
Spike Protein ◽  
Infection Model ◽  
Converting Enzyme ◽  
Viral Receptor ◽  
Angiotensin Converting Enzyme 2 ◽  
People With Dementia ◽  
The Impact

Increasing evidence suggests that elderly people with dementia are vulnerable to the development of severe coronavirus disease 2019 (COVID-19). In Alzheimer’s disease (AD), the major form of dementia, β-amyloid (Aβ) levels in the blood are increased; however, the impact of elevated Aβ levels on the progression of COVID-19 remains largely unknown. Here, our findings demonstrate that Aβ1-42, but not Aβ1-40, bound to various viral proteins with a preferentially high affinity for the spike protein S1 subunit (S1) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the viral receptor, angiotensin-converting enzyme 2 (ACE2). These bindings were mainly through the C-terminal residues of Aβ1-42. Furthermore, Aβ1-42 strengthened the binding of the S1 of SARS-CoV-2 to ACE2 and increased the viral entry and production of IL-6 in a SARS-CoV-2 pseudovirus infection model. Intriguingly, data from a surrogate mouse model with intravenous inoculation of Aβ1-42 show that the clearance of Aβ1-42 in the blood was dampened in the presence of the extracellular domain of the spike protein trimers of SARS-CoV-2, whose effects can be prevented by a novel anti-Aβ antibody. In conclusion, these findings suggest that the binding of Aβ1-42 to the S1 of SARS-CoV-2 and ACE2 may have a negative impact on the course and severity of SARS-CoV-2 infection. Further investigations are warranted to elucidate the underlying mechanisms and examine whether reducing the level of Aβ1-42 in the blood is beneficial to the fight against COVID-19 and AD.

Mixed methods-onderzoek om beweging en slaap van ouderen in kaart te brengen

KWALON ◽  
2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Julie Vanderlinden ◽  
Sabine Lambers ◽  
Reninka De Koker ◽  
Liza Musch
Keyword(s):  
Mixed Methods ◽  
Health Outcomes ◽  
Elderly People ◽  
Health Workers ◽  
The Elderly ◽  
Healthy Ageing ◽  
Physically Active ◽  
Know How ◽  
Measuring Methods

Mixed methods to measure movement and sleep in the elderly Movement and sleep are both crucial for the elderly in terms of healthy ageing. Staying physically active as people age is positively associated with better sleep and health outcomes. But despite the growing attention to healthy ageing, the elderly don’t always keep up with the applicable guidelines in terms of movement. Furthermore, health workers don’t always know how to measure movement and sleep in elderly in order to advise and help them to better adhere to guidelines. Literature lacks a summary of accessible objective and subjective ways to measure movement and sleep in the elderly. This study combines both objective and subjective measuring methods and elaborates on the practical aspects of their use. This paper highlights the advantages of mixed methods when measuring movement and sleep in elderly people and aims to inform health workers who want to evaluate their patients’ movement and sleep in order to guide them towards a healthier lifestyle.

PENGARUH SENAM JANTUNG SEHAT SERI-I TERHADAP DAYA TAHAN JANTUNG-PARU, KEKUATAN OTOT DAN KADAR TNF-? PLASMA PADA LANJUT USIA

2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Ajeng Fitria ◽  
Leonardo Lubis ◽  
Ambrosius Purba
Keyword(s):  
Muscle Strength ◽  
Health Workers ◽  
The Elderly ◽  
Moderate Intensity ◽  
P Value ◽  
Back Muscle ◽  
Back Muscle Strength ◽  
Shoulder Muscles ◽  
Before And After ◽  
Post Test

The increasing number of elderly and elderly life expectancy is a challenge for health workers. Decreased cardiac endurance, muscle strength and increased incidence of inflammation will occur in the elderly as we get older. health cardio exercises series-I is an aerobic exercise that has moderate intensity and is carried out for 30 minutes, research is needed to analyze the effect on cardiac endurance, muscle strength and decrease plasma TNF-? levels in the elderly. Research design with experimental method, pre-test and post-test design approach. The study was conducted for 4 weeks on 25 samples which were divided into 3 groups of frequency of health cardio exercises series-I to measure endurance of the heart. A 6-minute road test and muscle strength were used for hand grip strength dynamometer, test back and leg dynamometer. Measurement of plasma TNF-? levels is carried out using the Elisa method. The data was then analyzed using paired t-test to see differences before and after health cardio exercises series-I. The results showed health cardio exercises series-I performed 3x / mg can increase the heart's endurance compared to the frequency of 4x / mg and 5x / mg (-16.11 vs 15.62 vs -47.62 meters). health cardio exercises series-I frequency of 3x / mg and 4x / mg can increase back muscle strength (p-value = 0.019, p <0.05) and the frequency of 5x / mg can increase the strength of attractive shoulder muscles. (p-value = 0.033, p <0.05). Health cardio exercises series-I performed 3x / mg, 4x / mg and 5x / mg did not have a significant effect on plasma TNF-? protein expression in the elderly (0.506 vs 0.856 vs. 0.382, p <0.05). It is suggested that the elderly can do I-1 series heart exercise with a frequency of 3x / mg to increase the heart's lung endurance and back muscle strength, a health cardio exercises series-I 4x / mg series to increase muscle strength of the back back, a series of health cardio exercises series-I frequency of 5x / mg to increase attractive shoulder muscle strength.

scholarly journals Prevalence and factors associated with Metabolic Syndrome in elderly users of the Unified Health System

2014 ◽  
Vol 17 (4) ◽  
pp. 805-817 ◽  
Author(s):  
Edna Cunha Vieira ◽  
Maria do Rosário Gondim Peixoto ◽  
Erika Aparecida da Silveira
Keyword(s):  
Metabolic Syndrome ◽  
Health System ◽  
Health Workers ◽  
The Elderly ◽  
World Health ◽  
Fat Percentage ◽  
Cross Sectional ◽  
Factors Associated ◽  
Unified Health System ◽  
The Metabolic Syndrome

OBJECTIVE: To evaluate the prevalence and factors associated with metabolic syndrome in the elderly. METHODS: Cross-sectional study, with 133 individuals randomly selected in the Unified Health System in Goiania, Goiás. The following variables were researched: anthropometric (BMI, waist circumference, fat percentage by Dual X-ray absorptiometry), sociodemographic (gender, age, color, income, marital status and years of schooling), lifestyle (physical activity, smoking and risk alcohol consumption) and food intake (risk and protective foods). The metabolic syndrome was assessed according to harmonized criteria proposed by the World Health Organization (WHO). The combinations were tested by Poisson regression for confounding factors. RESULTS: The prevalence of metabolic syndrome was 58.65% (95%CI 49.8 - 67.1), with 60.5% (95%CI 49.01 - 71.18) for females and 55.7% (95%CI 41.33 - 69.53) for males. Hypertension was the most prevalent component of the syndrome in both men, with 80.8% (95%CI 64.5 - 90.4), and women, with 85.2% (95%CI 75.5 - 92.1). After the multivariate analysis, only the excess of weight measured by body mass index (prevalence ratio = 1.66; p < 0.01) remained associated with the metabolic syndrome. CONCLUSIONS: The prevalence of metabolic syndrome in this sample was high, indicating the need for systematic actions by health workers in the control of risk factors through prevention strategies and comprehensive care to the elderly.

scholarly journals Controlling of a ROS-based robotic system in accordance to the assist-as-needed principle in end-effector based rehabilitation systems

2018 ◽  
Vol 4 (1) ◽  
pp. 199-202
Author(s):  
Sebastian Becker ◽  
Wiebke Hinterlang ◽  
Tim Eschert ◽  
Catherine Disselhorst-Klug
Keyword(s):  
Impedance Control ◽  
Proximal Part ◽  
The Elderly ◽  
Subjective Assessment ◽  
Robot Kinematics ◽  
Robotic Rehabilitation ◽  
End Effector ◽  
3 Dimensional ◽  
The Central Nervous System ◽  
Robotic Devices

AbstractStroke is one of the most frequent diseases among the elderly and often leads to an ongoing failure of functions in the central nervous system. Due to the plasticity of the brain affected may regain lost motor function by repetitive training. Robotic devices can be an approach to accelerate the rehabilitation process by maximizing patients’ training intensity. End-effector based robotic systems are particularly suitable for this purpose and often an advantage over exoskeletons since the proximal part of the upper limb remains under the control of the patient. Furthermore, the integration of the assistas- needed principle (AAN) into these devices enables individualized, adaptable robotic support to patients during therapy. In this study an end-effector based robotic rehabilitation device based on the Robot Operating System (ROS) framework is introduced. The system allows patients to perform 3- dimensional movements without a therapist’s assistance. With regard to the AAN, focus was based on impedance control and an additional real-time adaption of the impedance control parameters by using a feedback loop. 10 healthy subjects took part in this study to evaluate the overall concept with regard to usability and quality of the supported movement. Hence, the three most promising adaption models of AAN (without adaption, adaption according to position and time, adaption according to velocity) under three different levels of movement support (0%, 50%, 100%) were investigated by administering a self-designed questionnaire and the robot kinematics. The results showed no significant differences between the three different adaption models of AAN. However, the subjective assessment of the movements was in keeping with robot kinematics and the control approaches as well as the overall system have experienced remarkable support.

scholarly journals Ansiedad, depresión y malestar emocional en los profesionales sanitarios de las Unidades de Cuidados Intensivos

2015 ◽  
Vol 31 (2) ◽  
pp. 743
Author(s):  
Sandra Gómez-Martínez ◽  
Rafael Ballester-Arnal ◽  
Beatriz Gil-Juliá ◽  
Ricardo Abizanda-Campos
Keyword(s):  
Mental Health ◽  
Gender Differences ◽  
Intensive Care ◽  
Health Workers ◽  
Anxiety And Depression ◽  
Subjective Perception ◽  
Depression And Anxiety ◽  
Regression Analyses ◽  
Daily Contact ◽  
Emotional Effects

<p class="textoteoradef">Health Workers (HW) who work in the Intensive Care Units (ICU), are not exempt from emotional effects of coping with suffering and death situations daily. Many studies in HW have focused on the <em>burnout</em> syndrome and have presented anxiety and depression as consequences of this syndrome. The aim of this work is to assess the anxiety and depression problems, as well as the subjective perception of mood of the ICU health workers. Participants were 117 ICU health workers of a Hospital in the Valencian Community (Spain). The descriptive analyses show that between 14.6-20% and a 4-7% of participants have symptoms of anxiety and depression, respectively. Differential analyses, show gender differences in several measures of anxiety, as well as on depression scores. Age and experience are related to depression and anxiety. Furthermore, we performed regression analyses to know the importance of the different variables in the presence of symptoms of anxiety and depression. The results show that working in daily contact with suffering and death, in a Unit in which the demands are many, could have implications for the mental health of professionals.</p>

scholarly journals Hyponatremia and Oxidative Stress

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1768
Author(s):  
Benedetta Fibbi ◽  
Giada Marroncini ◽  
Cecilia Anceschi ◽  
Laura Naldi ◽  
Alessandro Peri
Keyword(s):  
Oxidative Stress ◽  
Cancer Progression ◽  
The Elderly ◽  
Sodium Concentration ◽  
Electrolyte Imbalance ◽  
Common Denominator ◽  
Oncologic Patients ◽  
The Central Nervous System ◽  
Acute Hyponatremia ◽  
Neuronal Swelling

Hyponatremia, i.e. the presence of a serum sodium concentration ([Na+]) < 136 mEq/L, is the most frequent electrolyte imbalance in the elderly and in hospitalized patients. Symptoms of acute hyponatremia, whose main target is the central nervous system, are explained by the “osmotic theory” and the neuronal swelling secondary to decreased extracellular osmolality, which determines cerebral oedema. Following the description of neurological and systemic manifestations even in mild and chronic hyponatremia, in the last decade reduced extracellular [Na+] was associated with detrimental effects on cellular homeostasis independently of hypoosmolality. Most of these alterations appeared to be elicited by oxidative stress. In this review, we focus on the role of oxidative stress on both osmolality-dependent and -independent impairment of cell and tissue functions observed in hyponatremic conditions. Furthermore, basic and clinical research suggested that oxidative stress appears to be a common denominator of the degenerative processes related to aging, cancer progression, and hyponatremia. Of note, low [Na+] is able to exacerbate multiple manifestations of senescence and to decrease progression-free and overall survival in oncologic patients.

Sign in / Sign up

Export Citation Format

Share Document