scholarly journals Characterization of cDNA Encoding Resveratrol Synthase and Accumulation of Resveratrol in Tartary Buckwheat

2013 ◽  
Vol 8 (11) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Yeon Bok Kim ◽  
Aye Aye Thwe ◽  
YeJi Kim ◽  
Sun Kyung Yeo ◽  
Chanhui Lee ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Light Condition ◽  
Tartary Buckwheat ◽  
Dark Condition ◽  
Fagopyrum Tataricum ◽  
Reading Frame ◽  
Expression Levels ◽  
Resveratrol Synthase ◽  
Key Enzyme

Resveratrol synthase (RS) is the key enzyme for biosynthesis of resveratrol which come from coumaroyl-coenzyme A (CoA) and malonyl-CoA. Here, we report the cloning and characterization of a RS gene and accumulation of resveratrol in tartary buckwheat ( Fagopyrum tataricum). FtRS was composed of 1173 bp open reading frame and 390 amino acid residues and had a theoretical molecular weight and isoelectric point value of 43.70 kDa and 6.24, respectively. The FtRS expression levels were examined in sprouts and different organs of two tartary buckwheat cultivars, Hokkai T8 (T8) and Hokkai T10 (T10). FtRS transcript levels and resveratrol contents were higher under the dark condition compared with light condition. The expression levels of different organs of T10 was not observed significant variations compared to different organs of T8. Interestingly, resveratrol was detected in the sprouts developmental stages, but no resveratrol could not detect in any other organs of both T8 and T10. Therefore, we suggest that the resveratrol content in tartary buckwheat sprouts may be attributed mainly to the dark condition. The characterization of FtRS will be helpful for better understanding of the resveratrol biosynthesis in tartary buckwheat.

scholarly journals Cloning, Characterization, and RNA Interference Effect of the UDP-N-Acetylglucosamine Pyrophosphorylase Gene in Cnaphalocrocis medinalis

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 464
Author(s):  
Yuan-Jin Zhou ◽  
Juan Du ◽  
Shang-Wei Li ◽  
Muhammad Shakeel ◽  
Jia-Jing Li ◽  
...  
Keyword(s):  
Developmental Disorders ◽  
Developmental Stages ◽  
Phylogenetic Analyses ◽  
Digital Pcr ◽  
Cnaphalocrocis Medinalis ◽  
Chitin Synthesis ◽  
Reading Frame ◽  
Rnai Technology ◽  
Glyphodes Pyloalis ◽  
Key Enzyme

The rice leaf folder, Cnaphalocrocis medinalis is a major pest of rice and is difficult to control. UDP-N-acetylglucosamine pyrophosphorylase (UAP) is a key enzyme in the chitin synthesis pathway in insects. In this study, the UAP gene from C. medinalis (CmUAP) was cloned and characterized. The cDNA of CmUAP is 1788 bp in length, containing an open reading frame of 1464 nucleotides that encodes 487 amino acids. Homology and phylogenetic analyses of the predicted protein indicated that CmUAP shared 91.79%, 87.89%, and 82.75% identities with UAPs of Glyphodes pyloalis, Ostrinia furnacalis, and Heortia vitessoides, respectively. Expression pattern analyses by droplet digital PCR demonstrated that CmUAP was expressed at all developmental stages and in 12 tissues of C. medinalis adults. Silencing of CmUAP by injection of double-stranded RNA specific to CmUAP caused death, slow growth, reduced feeding and excretion, and weight loss in C. medinalis larvae; meanwhile, severe developmental disorders were observed. The findings suggest that CmUAP is essential for the growth and development of C. medinalis, and that targeting the CmUAP gene through RNAi technology can be used for biological control of this insect.

scholarly journals (86) Isolation and Molecular Characterization of Putative Ascorbate Peroxidase from Citrus

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1021D-1021
Author(s):  
Madhurababu Kunta ◽  
H. Sonia del Rio ◽  
Eliezer Louzada
Keyword(s):  
Ascorbate Peroxidase ◽  
Normal Growth ◽  
Stress Conditions ◽  
Full Length ◽  
Homology Search ◽  
Reading Frame ◽  
Key Enzyme ◽  
Differential Display Reverse Transcription ◽  
Polymerase Chain

Reactive oxygen species (ROS) are continuously produced during the normal aerobic metabolism and also under environmental stress conditions. They are the major damaging factors to the photosynthetic machinery under stress conditions and need to be scavenged for the normal growth of the plant. Ascorbate peroxidase (APX) is the key enzyme in detoxifying H2O2, one of ROS from chloroplast and cytosol. A cDNA encoding a putative APXcit was isolated from mature `Dancy' tangerine (Citrus reticulata Blanco) juice vesicles using differential display reverse transcription-polymerase chain reaction (RT-PCR). Subsequently, full-length APXcit cDNA clone and genomic clone were obtained and sequenced. The full-length APXcit sequence is composed by 1082-bp nucleotides, including an open reading frame (ORF) of 753 bp, encoding a protein of 250 amino acids (27 kDa). The 5' un-translated region (UTR) of the APXcit gene consisted of 91 nucleotides and the 3' UTR consisted of 238 nucleotides. Homology search for APXcit at GenBank database showed high similarity to APX from several plant species.

scholarly journals Overexpression of Trypanosoma rangeli trypanothione reductase increases parasite survival under oxidative stress

2016 ◽  
Vol 2 ◽  
Author(s):  
I.T. BELTRAME-BOTELHO ◽  
P.H. STOCO ◽  
M. STEINDEL ◽  
B. ANDERSSON ◽  
E.F. PELOSO ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Functional Characterization ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Single Copy ◽  
Trypanothione Reductase ◽  
Trypanosoma Rangeli ◽  
Reading Frame ◽  
Key Enzyme

SUMMARYThe infectivity and virulence of pathogenic trypanosomatids are directly associated with the efficacy of their antioxidant system. Among the molecules involved in the trypanosomatid response to reactive oxygen or nitrogen species, trypanothione reductase (TRed) is a key enzyme. In this study, we performed a molecular and functional characterization of the TRed enzyme fromTrypanosoma rangeli(TrTRed), an avirulent trypanosome of mammals. TheTrTRed gene has an open reading frame (ORF) of 1473 bp (~490 aa, 53 kDa) and occurs as a single-copy gene in the haploid genome. The predicted protein contains two oxidoreductase domains, which are equally expressed in the cytosol of epimastigotes and trypomastigotes. Nicotinamide adenine dinucleotide phosphate (NADPH) generation is reduced and endogenous H2O2production is elevated inT. rangeliChoachí strain compared withT. cruziY strain epimastigotes. Oxidative stress induced by H2O2does not induce significant alterations inTrTRed expression. Overexpression ofTrTRed did not influencein vitrogrowth or differentiation into trypomastigotes, but mutant parasites showed increased resistance to H2O2-induced stress. Our results indicate thatT. rangeliconstitutively expresses TRed during the entire life cycle, with reduced levels during infective and non-replicative trypomastigote stages.

Cloning and characterization of aCOBRA-like gene expressedde novoduring maize germination

2003 ◽  
Vol 13 (3) ◽  
pp. 209-217 ◽  
Author(s):  
Felipe Cruz-García ◽  
Alberto Gómez ◽  
José Juan Zúñiga ◽  
Javier Plasencia ◽  
Jorge M. Vázquez-Ramos
Keyword(s):  
Cell Expansion ◽  
Developmental Stages ◽  
Mrna Differential Display ◽  
Reading Frame ◽  
Early Germination ◽  
Embryo Axes ◽  
Leaf Tissues ◽  
Root Tissues ◽  
Tissues Expression

AbstractThe search for germination-specific genes has been a laborious and unrewarding task, since many of the genes expressed during germination are also expressed in embryogenesis or in other developmental stages. By using mRNA differential display of transcript populations from maize (Zea maysL.) embryo axes, germinated for different times with or without a previous osmopriming treatment, a 682 bp cDNA was isolated that was present only after 24 h germination, and absent during osmopriming or during early germination. Screening of a cDNA library using the 682 bp probe yielded a 1554 bp cDNA that contained an open reading frame coding for 436 amino acids. This gene, referred to asZmAA9-24, was expressed in root tissues, but was not detected in shoot or leaf tissues. Expression ofZmAA9-24occurred earlier during germination (by 15 h) if embryo axes were imbibed in the presence of cytokinins or if seeds were previously osmoprimed. The predicted protein sequence ofZmAA9-24is 39.6% identical to the product of the recently identifiedArabidopsisgeneCOBRA(54.5% in the central region), which appears to participate in the regulation of cell expansion, particularly in roots, and belongs to the glycosylphosphatidylinositol (GPI)-anchored protein family.ZmAA9-24expression might be regulated by both cell expansion and the cell cycle, processes that have a central role during seed germination.

scholarly journals Identification and Characterization of A Lanosterol synthase Gene from Sanghuangporus Baumii

2021 ◽  
Author(s):  
XuTong Wang ◽  
TingTing Sun ◽  
Jian Sun ◽  
Zengcai Liu ◽  
Li Zou
Keyword(s):  
Untranslated Region ◽  
Mevalonate Pathway ◽  
Promoter Sequence ◽  
Mva Pathway ◽  
Reading Frame ◽  
E Coli ◽  
Lanosterol Synthase ◽  
Key Enzyme ◽  
Rapid Amplification

Abstract Lanosterol synthase (LS) is a key enzyme involved in the mevalonate pathway (MVA pathway) to produce lanosterol, which is a precursor for synthesizing Sanghuangporus baumii triterpenoids. To research the characteristics and construction of LS, LS ORF and promoter were cloned from S. baumii. A 2,445 bp S. baumii LS sequence was obtained by rapid amplification of cDNA ends (RACE) technology and recombinant PCR. S. baumii LS sequence includes a 5’-untranslated region (129 bp), a 3’-untranslated region (87 bp), and an open reading frame (2,229 bp) encoding a 734 amino acids. The molecular weight of LS is 84.99 kDa, and transcription start site of S. baumii LS promoter sequence ranged from 1 740 bp to 1790 bp. LS promoter contained 12 CAAT-boxes, 5 ABREs, 6 G-Boxes, 6 CGTCA-motifs, and so on. The S. baumii LS protein was expressed in E. coli BL21 (DE3) (84.99 kDa + 21.15 kDa tag protein). The transcription level of S. baumii LS was the highest on day 11 in mycelia (1.6-fold).

scholarly journals Characterization of Rutin-rich Bread Made with ‘Manten-Kirari’, a Trace-rutinosidase Variety of Tartary Buckwheat (Fagopyrum tataricum Gaertn.)

2015 ◽  
Vol 21 (5) ◽  
pp. 733-738 ◽  
Author(s):  
Tatsuro Suzuki ◽  
Toshikazu Morishita ◽  
Shigenobu Takigawa ◽  
Takahiro Noda ◽  
Koji Ishiguro
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

scholarly journals Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat (Fagopyrum tataricum)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hongyou Li ◽  
Hengling Meng ◽  
Xiaoqian Sun ◽  
Jiao Deng ◽  
Taoxiong Shi ◽  
...  
Keyword(s):  
Seed Development ◽  
Target Genes ◽  
Developmental Stages ◽  
Transcriptome Profiling ◽  
Mineral Elements ◽  
Endosperm Development ◽  
Tartary Buckwheat ◽  
Integrated Analysis ◽  
Fagopyrum Tataricum ◽  
Regulatory Pathways

Abstract Background Tartary buckwheat seed development is an extremely complex process involving many gene regulatory pathways. MicroRNAs (miRNAs) have been identified as the important negative regulators of gene expression and performed crucial regulatory roles in various plant biological processes. However, whether miRNAs participate in Tartary buckwheat seed development remains unexplored. Results In this study, we first identified 26 miRNA biosynthesis genes in the Tartary buckwheat genome and described their phylogeny and expression profiling. Then we performed small RNA (sRNA) sequencing for Tartary buckwheat seeds at three developmental stages to identify the miRNAs associated with seed development. In total, 230 miRNAs, including 101 conserved and 129 novel miRNAs, were first identified in Tartary buckwheat, and 3268 target genes were successfully predicted. Among these miRNAs, 76 exhibited differential expression during seed development, and 1534 target genes which correspond to 74 differentially expressed miRNAs (DEMs) were identified. Based on integrated analysis of DEMs and their targets expression, 65 miRNA-mRNA interaction pairs (25 DEMs corresponding to 65 target genes) were identified that exhibited significantly opposite expression during Tartary buckwheat seed development, and 6 of the miRNA-mRNA pairs were further verified by quantitative real-time polymerase chain reaction (qRT-PCR) and ligase-mediated rapid amplification of 5′ cDNA ends (5′-RLM-RACE). Functional annotation of the 65 target mRNAs showed that 56 miRNA-mRNA interaction pairs major involved in cell differentiation and proliferation, cell elongation, hormones response, organogenesis, embryo and endosperm development, seed size, mineral elements transport, and flavonoid biosynthesis, which indicated that they are the key miRNA-mRNA pairs for Tartary buckwheat seed development. Conclusions Our findings provided insights for the first time into miRNA-mediated regulatory pathways in Tartary buckwheat seed development and suggested that miRNAs play important role in Tartary buckwheat seed development. These findings will be help to study the roles and regulatory mechanism of miRNAs in Tartary buckwheat seed development.

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