scholarly journals Total Oxidant and Antioxidant Capacity in Patients with Transfusion Dependent and Nondependent Beta Thalassemia

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4573-4573
Author(s):  
Zeynep Karakas ◽  
Yasin Yilmaz ◽  
Dolay Damla Celik ◽  
Agageldi Annayev ◽  
Selcan Demirel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Conflicts Of Interest ◽  
Beta Thalassemia ◽  
Control Group ◽  
Simple Method ◽  
Total Antioxidant

Abstract Background: The patients with β-thalassemia are usually under oxidative stress due to iron overload as a result of ineffective erythropoiesis and repeated transfusions. The endogen (uric acid, indirect bilirubin) and exogenous (ascorbic acid and vitamin E) antioxidants protect the cells from oxidative damage. The evaluation of antioxidant defense system can be easily done by measuring serum total antioxidant capacity. The aim of this study is to investigate the total oxidant (TOC) and antioxidant capacity (TAC) of patients with transfusion dependent (TDT) and nondependent (NTDT) β -thalassemia. Methods: Fifty eight patients (age range 3-59; median age 24 years) with β-thalassemia (16 nontransfusion dependent, 42 transfusion dependent) who were followed-up by Istanbul Medical Faculty Thalassemia Center were enrolled in this study. The sex and age matched 25 healthy subjects were used as control group. The total oxidant and antioxidant capacity were measured by Rel Assay Diagnostics, Total Oxidant Status (TOS) kit and Total Antioxidant Status (TAS) kit. The statistics of results were done by SPSS version 21. Results: 58 patients were included consisting of β thalassemia intermedia (n=16) and β thalassemia major (n=42). The total oxidant capacity was found higher in patients than control group without significant differences. Beside this, the antioxidant capacity of patients was significantly increased (2.75 mmol/l vs 1.94 mmol/l; p=0.001). Within patients group, there was no significant differences in terms of TAC while the TOC level was high in NTDT (2.99 mmol/l vs 1.88 mmol/l; p=0.08). There was no significant relationship between TOC and sex, age (<18 vs >18 years), ferritin and splenectomy status, as the same for TAC. Conclusions: The oxidative damage in patients with β-thalassemia can trigger the morbidity. The measurement of TOC and TAC status stands a useful, rapid, and simple method to evaluate the complex oxidative mechanism of disease. There are still controversial results about antioxidant status in thalassemia patients. The irregular consume of supportive treatment such as zinc, vitamin C, vitamin E and herbal medicine may affect the TOC and TAC status. The results of this study can guide us to use the antioxidants to decrease the oxidative stress. Figure 1. Figure 1. Disclosures No relevant conflicts of interest to declare.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals Blood Levels of Oxidant/Antioxidant Parameters in Rats Infected withToxoplasma gondii

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Somayeh Bahrami ◽  
Ali Shahriari ◽  
Mehdi Tavalla ◽  
Somayeh Azadmanesh ◽  
Hossein Hamidinejat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Parasitic Infection ◽  
Chronic Phase ◽  
Antioxidant Defense System ◽  
Female Rats ◽  
Control Group ◽  
Blood Levels ◽  
Total Antioxidant

Toxoplasmosis is a common parasitic infection in the world. Since increased free radicals and oxidative stress are reported in many parasitic diseases the purpose of the present study was to evaluate the oxidative stress in acute and chronic toxoplasmosis. RH strains ofToxoplasmatachyzoites were used in the present study. Twenty-five female rats were infected with the parasite while 25 other rats were as the control group that received normal saline. Zero-, 5-, 7-, 10-, and 45-day postinfection (DPI) blood samples were taken. Some parameters related to oxidant and antioxidants such as antioxidant enzymes, malondialdehyde, and total antioxidant capacity were measured. On day 7 after infection, GPX activity and GSH level were significantly increased and in the mentioned day the amount of total antioxidant capacity was significantly reduced. In other cases, there were no significant differences between the groups in different days. Overall, based on the results it seems that, on day 7 after infection, in infected rats responses to oxidative stress were triggered and led to decrease of total antioxidant capacity. Furthermore, glutathione was increased to cope with stress. It seems that probably antioxidant defense system entered the infection to the chronic phase and changed the parasites stage.

scholarly journals Gebe Ratlara uygulanan Akrilamid ve E Vitaminin Plasenta Dokusu Üzerine Olası Etkilerinin Araştırılması

2017 ◽  
Vol 5 (4) ◽  
pp. 349
Author(s):  
Mehmet Erman Erdemli ◽  
Eyüp Altınöz ◽  
Zeynep Aksungur ◽  
Zümrüt Doğan ◽  
Harika Gözükara Bağ ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Total Antioxidant Capacity ◽  
Corn Oil ◽  
Reduced Glutathione ◽  
Control Group ◽  
Vaginal Smear ◽  
Protective Agent ◽  
Pregnant Rats ◽  
Total Antioxidant

Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.

scholarly journals Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

2017 ◽  
Vol 45 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Andrei CONEAC ◽  
Meda Sandra ORASAN ◽  
Daniel Corneliu LEUCUTA ◽  
Nicoleta DECEA ◽  
Miuta FILIP ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Experimental Inflammation ◽  
Group Ii

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

scholarly journals Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents

2020 ◽  
Vol 9 (2) ◽  
pp. 548 ◽  
Author(s):  
Anna Zalewska ◽  
Agnieszka Kossakowska ◽  
Katarzyna Taranta-Janusz ◽  
Sara Zięba ◽  
Katarzyna Fejfer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Redox Homeostasis ◽  
Salivary Secretion ◽  
Control Group ◽  
Total Protein Content ◽  
Parotid Glands ◽  
Obese Adolescents ◽  
Total Antioxidant ◽  
And Gender

Obesity is inseparably connected with oxidative stress. This process may disturb the functioning of the oral cavity, although the effect of oxidative stress on salivary gland function and changes in the qualitative composition of saliva are still unknown. Our study is the first to evaluate salivary redox homeostasis in 40 overweight and obese adolescents and in the age- and gender-matched control group. We demonstrated strengthening of the antioxidant barrier (superoxide dismutase, catalase, peroxidase, uric acid, total antioxidant capacity (TAC)) with a simultaneous decrease in reduced glutathione concentration in saliva (non-stimulated/stimulated) in overweight and obese teenagers compared to the controls. The concentration of the products of oxidative damage to proteins (advanced glycation end products), lipids (malondialdehyde, 4-hydroxynonenal) and DNA (8-hydroxydeoxyguanosine) as well as total oxidative status were significantly higher in both non-stimulated and stimulated saliva as well as plasma of overweight and obese adolescents. Importantly, we observed more severe salivary and plasma redox alterations in obese adolescents compared to overweight individuals. In the study group, we also noted a drop in stimulated salivary secretion and a decrease in total protein content. Interestingly, dysfunction of parotid glands in overweight and obese teenagers intensified with the increase of BMI. We also showed that the measurement of salivary catalase and TAC could be used to assess the central antioxidant status of overweight and obese adolescents.

scholarly journals Nonenzymatic Serum Antioxidant Capacity in IBD and Its Association with the Severity of Bowel Inflammation and Corticosteroids Treatment

Medicina ◽  
2019 ◽  
Vol 55 (4) ◽  
pp. 88 ◽  
Author(s):  
Katarzyna Neubauer ◽  
Radoslaw Kempinski ◽  
Malgorzata Matusiewicz ◽  
Iwona Bednarz-Misa ◽  
Malgorzata Krzystek-Korpacka
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Uric Acid ◽  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Mucosal Healing ◽  
Extraintestinal Manifestation ◽  
Total Antioxidant ◽  
History Of

Background and objectives: Oxidative stress signalling plays a monumental role in inflammatory bowel disease (IBD). Reduction of oxidative stress might control inflammation, block tissue damage, and reverse natural history of IBD. We assessed the serum concentrations of free thiols (FT) and uric acid (SUA), together constituting a large part of nonenzymatic serum antioxidant capacity, as well as total antioxidant status (TAS) with reference to IBD phenotype, activity, co-occurrence of anemia, and treatment with azathioprine (AZA) and corticosteroids (CS). Additionally, we appraised the potential of uric acid, thiol stress, and TAS as mucosal healing (MH) markers in ulcerative colitis. Materials and methods: SUA, FT, and TAS were measured colorimetrically using, respectively, uricase, Ellman’s and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) methods. Results: The study group consisted of 175 individuals: 57 controls, 71 ulcerative colitis (UC), and 47 Crohn’s disease (CD) patients. When compared to controls, SUA levels were significantly lower in patients with CD, and FT and TAS levels were significantly lower in patients with CD and UC. In UC patients, SUA, FT, and TAS inversely correlated with the severity of bowel inflammation. As MH markers, SUA displayed better overall accuracy and higher specificity than FT. In active CD, FT, and SUA were significantly lower in patients with anemia. FT was significantly lower in patients treated with corticosteroids. Conclusions: IBD patients, regardless the disease phenotype, have systemic thiol stress, depleted total antioxidant capacity, and reduced concentrations of uric acid, reflecting, to various degrees, clinical and local disease activity as well as presence of anaemia, the most common extraintestinal manifestation of IBD. Evaluation of systemic total antioxidant status may be useful in noninvasive assessment of mucosal healing. Our findings on thiol stress provide an additional aspect on adverse effects of corticosteroids therapy.

scholarly journals The Association between Diabetic Retinopathy and Levels of Ischemia-Modified Albumin, Total Thiol, Total Antioxidant Capacity, and Total Oxidative Stress in Serum and Aqueous Humor

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kadir Kirboga ◽  
Ayse V. Ozec ◽  
Mustafa Kosker ◽  
Ayhan Dursun ◽  
Mustafa I. Toker ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Antioxidant Capacity ◽  
Aqueous Humor ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Thiol ◽  
Median Serum ◽  
Total Antioxidant ◽  
Total Oxidative Stress

Purpose. To investigate the oxidant and antioxidant status of patients with type 2 diabetes mellitus and nonproliferative diabetic retinopathy (DRP).Methods. Forty-four patients who had cataract surgery were enrolled in the study. We included 22 patients with DRP in one group and 22 patients in the control group. Samples of aqueous humor and serum were taken from all patients. Serum and aqueous ischemia-modified albumin (IMA), total thiol, total antioxidant capacity (TAC), and total oxidative stress (TOS) levels were compared in two groups.Results. Median serum IMA levels were 44.80 absorbance units in the DRP group and 40.15 absorbance units in the control group (P=0.031). Median serum total thiol levels in the DRP group were significantly less than those in the control group (3051.13 and 3910.12, resp.,P=0.004). Mean TOS levels in the serum were 2.93 ± 0.19 in the DRP group and 2.61 ± 0.26 in the control group (P=0.039). The differences in mean total thiol, TAC, and TOS levels in the aqueous humor and mean TAC levels in the serum were not statistically significant.Conclusion. IMA, total thiol, and TOS levels in the serum might be useful markers in monitoring the risk of DRP development.

Oxidative stress increases in carbon monoxide poisoning patients

2010 ◽  
Vol 30 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Havva Sahin Kavakli ◽  
Ozcan Erel ◽  
Orhan Delice ◽  
Gizem Gormez ◽  
Semra Isikoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Antioxidant Status ◽  
Carbon Monoxide Poisoning ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Parameters ◽  
Total Antioxidant ◽  
Oxidant Status

Objective: Carbon monoxide poisoning (COP) is one of the important causes of morbidity and mortality in toxicological cases. In this study, we aimed to find out more about the pathophysiology of COP by investigating the effects of COP on oxidative stress parameters such as total oxidant status (TOS) and total antioxidant status (TAS). Methods: Eighty-eight patients admitted to the emergency department of our hospital with acute COP and 35 healthy adults as control group were included in this study. Blood samples were collected from all COP patients at the time of initial emergency department evaluation to determine the oxidative stress parameters. Then, serum levels of total antioxidant status and total oxidant status levels were measured. Results: A total of 88 patients poisoned by carbon monoxide (CO; mean age 37.1 ± 18.2 years; 54’% women) were enrolled. TOS and carboxyhemoglobin (COHb) levels in COP patients were increased when compared to control group (p = 0.001). TOS, oxidative stress index (OSI) and COHb levels in COP patients were significantly lower after the treatment. (respectively, p = 0.016; p = 0.023; p = 0.001). On the other hand, no statistical differences were observed in TAS levels of study and control group as well as there were no changes with treatment. Conclusion: Measurements of TOS, TAS and OSI levels may be useful markers to find out the pathophsiology of COP.

scholarly journals Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver

2016 ◽  
Vol 66 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Marija Stojanović ◽  
Ljiljana Šćepanović ◽  
Olivera Bosnić ◽  
Dušan Mitrović ◽  
Olga Jozanov-Stankov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Defense Mechanism ◽  
Total Antioxidant Status ◽  
Male Rats ◽  
Control Group ◽  
Acute Administration ◽  
Rat Intestine ◽  
Homocysteine Thiolactone ◽  
Total Antioxidant

AbstractOxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p<0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p<0.001. Exposition to high D,L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p<0.01). Homocysteine also modifi ed the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p<0.001). Homocysteine induced a consistent oxidative stress in rat’s intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.

scholarly journals Sesame Meal, Vitamin E and Selenium Influence Goats’ Antioxidant Status

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 392
Author(s):  
Eleni Tsiplakou ◽  
Christina Mitsiopoulou ◽  
Chrysoula Karaiskou ◽  
Marica Simoni ◽  
Athanasios C. Pappas ◽  
...  
Keyword(s):  
Vitamin E ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Oxidative Status ◽  
Protein Carbonyls ◽  
Control Group ◽  
Sesame Meal ◽  
Total Antioxidant ◽  
Mda Content ◽  
The Impact

This study aimed to determine the impact of sesame meal, selenium (Se), and vitamin E (VitE) on goats’ oxidative status. Thirty mid-lactation crossbred goats were divided into five homogeneous groups, and were fed 1 kg of alfalfa hay and 1.2 kg of concentrates daily. The control group (C) received a basal diet. In the concentrates of the treated groups, 10% of the soybean meal was replaced by sesame meal and no extra VitE or Se (SM), or an extra 60 mg of VitE (SME), or 0.1 mg organic Se (SMSe), or their combination (60 mg VitE and 0,1 mg organic Se/kg of concentrate (SMESe). In the plasma of the goats, the dietary treatments did not affect glutathione reductase, glutathione peroxidase, glutathione transferase, catalase, superoxide dismutase activities, malondialdehyde (MDA) content, or the total antioxidant capacity. A reduction and a trend for lower protein carbonyls content was found in goats fed SM (p = 0.03) and SME (p = 0.06) compared to SMESe. In the milk, the lactoperoxidase activity decreased with SMSe and SMESe. A numerical decrease in the total antioxidant capacity and an increase in the MDA content in the milk of the SMESe group compared with the other treated groups was found. In mid-lactation goats, SM improves the oxidative status of both the organism and the milk.

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