scholarly journals IN VITRO STUDY OF BONE MARROW

Blood ◽  
1947 ◽  
Vol 2 (Special_Issue_Number_1) ◽  
pp. 33-41 ◽  
Author(s):  
CLAUS MUNK PLUM

Abstract Two types of apparatus are described for the culture of bone marrow in vitro. The first, a macro method, is suited to the study of large amounts of bone marrow. The second, a micro method, allows observations of the development of individual cells. Studies of erythropoiesis by these technics has allowed insight into production of erythrocytes. These data will be reported in a subsequent article.

2017 ◽  
Vol 357 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Long-Wei Hu ◽  
Xiao Wang ◽  
Xin-Qun Jiang ◽  
Li-Qun Xu ◽  
Hong-Ya Pan

2010 ◽  
Vol 6 (6) ◽  
pp. 2254-2263 ◽  
Author(s):  
M. Magallanes-Perdomo ◽  
A.H. De Aza ◽  
A.Y. Mateus ◽  
S. Teixeira ◽  
F.J. Monteiro ◽  
...  

1995 ◽  
Vol 48 (3) ◽  
pp. 243-247 ◽  
Author(s):  
CHIYUKI KAWASAKI ◽  
SEIICHI OKAMURA ◽  
KAZUYA SHIMODA ◽  
KYUICHI NEMOTO ◽  
YOSHIYUKI NIHO

2013 ◽  
Vol 132 (1) ◽  
pp. 70e-80e ◽  
Author(s):  
Akishige Hokugo ◽  
Sarah Sorice ◽  
Anisa Yalom ◽  
James C. Lee ◽  
Andrew Li ◽  
...  

2019 ◽  
Vol 08 (01) ◽  
pp. 1 ◽  
Author(s):  
A. Giai Via ◽  
M.B. McCarthy ◽  
M. Francke ◽  
F. Oliva ◽  
A.D. Mazzocca ◽  
...  

Blood ◽  
1947 ◽  
Vol 2 (Special_Issue_Number_1) ◽  
pp. 42-53 ◽  
Author(s):  
CLAUS MUNK PLUM

Abstract Certain problems in erythrocytogenesis have been studied by means of the in vitro culture of bone marrow. A few conclusions have been reached by this method: 1. Liver extract stimulates production of mature erythrocytes. 2. Plasma contains substances which stimulate the production of mature erythrocytes. 3. Plasma in anemic individuals (anemia of bleeding) probably contains substances which stimulate the production of mature erythrocytes; and the greater the anemia, the greater the stimulation. 4. Mitoses do not account for the production of mature erythrocytes from erythroblasts. Erythropoiesis rather occurs in two stages, in the first of which mitosis is of prime importance, and in the second of which gemmation of erythroblasts is the main mechanism.


Blood ◽  
1948 ◽  
Vol 3 (2) ◽  
pp. 155-164 ◽  
Author(s):  
JOHANNES CLEMMESEN ◽  
TAGE ESPERSEN ◽  
CLAUS MUNK PLUM

Abstract Experiments have been performed on sternal marrow kept alive in vitro with serum as a nutritional medium. Cross experiments with marrow and serum from two normal persons show roughly identical values for erythropoiesis. The same applies to marrows and sera from cases of mild simple anemia. Marrow from a patient with pernicious anemia showed increased erythropoiesis in vitro if nourished on normal serum, whereas serum from the same case lowered the erythropoiesis of normal marrow. After treatment with liver extract, conditions tended to return to normal. Marrow from 2 cases of lymphatic leukosis displayed corresponding phenomena, and showed a tendency to return to normal after treatment with urethane and folic acid. Further investigations along these lines are in progress.


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