scholarly journals IN VITRO STUDY OF BONE MARROW. III. ERYTHROPOIESIS IN VITRO OF STERNAL MARROW FROM CASES OF PERNICIOUS ANEMIA AND LYMPHATIC LEUKOSIS UNDER THERAPY

Blood ◽  
1948 ◽  
Vol 3 (2) ◽  
pp. 155-164 ◽  
Author(s):  
JOHANNES CLEMMESEN ◽  
TAGE ESPERSEN ◽  
CLAUS MUNK PLUM
Keyword(s):  
Bone Marrow ◽  
Folic Acid ◽  
Pernicious Anemia ◽  
Liver Extract ◽  
In Vitro Study ◽  
Normal Serum ◽  
Vitro Study ◽  
Nutritional Medium

Abstract Experiments have been performed on sternal marrow kept alive in vitro with serum as a nutritional medium. Cross experiments with marrow and serum from two normal persons show roughly identical values for erythropoiesis. The same applies to marrows and sera from cases of mild simple anemia. Marrow from a patient with pernicious anemia showed increased erythropoiesis in vitro if nourished on normal serum, whereas serum from the same case lowered the erythropoiesis of normal marrow. After treatment with liver extract, conditions tended to return to normal. Marrow from 2 cases of lymphatic leukosis displayed corresponding phenomena, and showed a tendency to return to normal after treatment with urethane and folic acid. Further investigations along these lines are in progress.

scholarly journals IN VITRO STUDY OF BONE MARROW

Blood ◽  
1947 ◽  
Vol 2 (Special_Issue_Number_1) ◽  
pp. 42-53 ◽  
Author(s):  
CLAUS MUNK PLUM
Keyword(s):  
Bone Marrow ◽  
In Vitro Culture ◽  
Liver Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Main Mechanism ◽  
Two Stages

Abstract Certain problems in erythrocytogenesis have been studied by means of the in vitro culture of bone marrow. A few conclusions have been reached by this method: 1. Liver extract stimulates production of mature erythrocytes. 2. Plasma contains substances which stimulate the production of mature erythrocytes. 3. Plasma in anemic individuals (anemia of bleeding) probably contains substances which stimulate the production of mature erythrocytes; and the greater the anemia, the greater the stimulation. 4. Mitoses do not account for the production of mature erythrocytes from erythroblasts. Erythropoiesis rather occurs in two stages, in the first of which mitosis is of prime importance, and in the second of which gemmation of erythroblasts is the main mechanism.

In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells

2017 ◽  
Vol 357 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Long-Wei Hu ◽  
Xiao Wang ◽  
Xin-Qun Jiang ◽  
Li-Qun Xu ◽  
Hong-Ya Pan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelin 1

scholarly journals ZnO folic acid conjugated nano-particles induce apoptosis on glioblastoma U-87MG tumor cell: An in vitro study

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S474
Author(s):  
Seyed Behnamedin Jameie ◽  
Mona Farhadi ◽  
Marfavi Zahra ◽  
Vahid Pirhajati ◽  
Melikasadt Jameie ◽  
...  
Keyword(s):  
Folic Acid ◽  
Tumor Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Nano Particles

scholarly journals Glioblastoma U-87MG tumour cells suppressed by ZnO folic acid-conjugated nanoparticles: an in vitro study

2019 ◽  
Vol 47 (1) ◽  
pp. 2783-2790 ◽  
Author(s):  
Zahra Hamod Marfavi ◽  
Mona Farhadi ◽  
Seyed Behnamedin Jameie ◽  
Masoomeh Zahmatkeshan ◽  
Vahid Pirhajati ◽  
...  
Keyword(s):  
Folic Acid ◽  
In Vitro Study ◽  
Tumour Cells ◽  
Vitro Study

In vitro study of the proliferation and growth of human bone marrow cells on apatite–wollastonite-2M glass ceramics

2010 ◽  
Vol 6 (6) ◽  
pp. 2254-2263 ◽  
Author(s):  
M. Magallanes-Perdomo ◽  
A.H. De Aza ◽  
A.Y. Mateus ◽  
S. Teixeira ◽  
F.J. Monteiro ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Glass Ceramics ◽  
In Vitro Study ◽  
Human Bone ◽  
Vitro Study ◽  
Human Bone Marrow ◽  
Marrow Cells

The effect of oxidative stress induced by tert-butylhydroperoxide under distinct folic acid conditions: An in vitro study using cultured human trophoblast-derived cells

2018 ◽  
Vol 77 ◽  
pp. 33-42 ◽  
Author(s):  
Jaqueline Vieira Carletti ◽  
Ana Correia-Branco ◽  
Claudia Raquel Silva ◽  
Nelson Andrade ◽  
Lenir Orlandi Pereira Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Folic Acid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Trophoblast ◽  
Tert Butylhydroperoxide

scholarly journals IN VITRO STUDY OF BONE MARROW

Blood ◽  
1947 ◽  
Vol 2 (Special_Issue_Number_1) ◽  
pp. 33-41 ◽  
Author(s):  
CLAUS MUNK PLUM
Keyword(s):  
Bone Marrow ◽  
In Vitro Study ◽  
Vitro Study ◽  
Insight Into

Abstract Two types of apparatus are described for the culture of bone marrow in vitro. The first, a macro method, is suited to the study of large amounts of bone marrow. The second, a micro method, allows observations of the development of individual cells. Studies of erythropoiesis by these technics has allowed insight into production of erythrocytes. These data will be reported in a subsequent article.

scholarly journals Microspectrophotometric Determination of Desoxyribosenucleic Acid in Megaloblasts of Pernicious Anemia

Blood ◽  
1951 ◽  
Vol 6 (4) ◽  
pp. 344-349 ◽  
Author(s):  
EDWARD H. REISNER ◽  
ROY KORSON
Keyword(s):  
Bone Marrow ◽  
Folic Acid ◽  
Vitamin B12 ◽  
Pernicious Anemia ◽  
Blood Cells ◽  
Nuclear Dna ◽  
Liver Extract ◽  
Megaloblastic Anemia ◽  
Gradual Loss

Abstract 1. In 9 patients with various types of megaloblastic anemia responding to treatment with vitamin B12, folic acid or liver extract, no significant deviations from the normal amounts of total or polymerized DNA were observed in the nuclei of red blood cells in marrow smears. 2. During the maturation of megaloblasts in the bone marrow there is a gradual loss of nuclear DNA. 3. This pattern is quantitatively and qualitatively similar for normal marrow and for that of pernicious anemia in relapse and after treatment.

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