scholarly journals Rearrangement of immunoglobulin heavy chain genes in T cell acute lymphoblastic leukemia

Blood ◽  
1985 ◽  
Vol 65 (3) ◽  
pp. 725-729 ◽  
Author(s):  
GR Kitchingman ◽  
U Rovigatti ◽  
AM Mauer ◽  
S Melvin ◽  
SB Murphy ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Heavy Chain ◽  
Germ Line ◽  
Lymphoblastic Leukemia ◽  
Immunoglobulin Heavy Chain ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Cell Acute Lymphoblastic Leukemia ◽  
Immunoglobulin Heavy Chain Genes

Abstract We studied the arrangement of the immunoglobulin heavy chain genes by Southern blot analysis of DNA freshly obtained from marrow blast cells of 14 children with T cell acute lymphoblastic leukemia (T-ALL) using probes to the C mu and JH gene segments: At least one of the C mu-gene alleles was rearranged in three cases. In two of these, one C mu gene had the germ-line configuration and one was rearranged, whereas both alleles were rearranged in the third case. In one case, a rearranged heavy chain gene hybridized to the C mu-region probe, but not to the JH probe, indicating that the entire JH region had been deleted. These results demonstrate that immunoglobulin heavy chain gene rearrangements are not restricted to B lineage lymphoproliferative diseases in humans.

scholarly journals Rearrangement of immunoglobulin heavy chain genes in T cell acute lymphoblastic leukemia

Blood ◽  
1985 ◽  
Vol 65 (3) ◽  
pp. 725-729
Author(s):  
GR Kitchingman ◽  
U Rovigatti ◽  
AM Mauer ◽  
S Melvin ◽  
SB Murphy ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Heavy Chain ◽  
Germ Line ◽  
Lymphoblastic Leukemia ◽  
Immunoglobulin Heavy Chain ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Cell Acute Lymphoblastic Leukemia ◽  
Immunoglobulin Heavy Chain Genes

We studied the arrangement of the immunoglobulin heavy chain genes by Southern blot analysis of DNA freshly obtained from marrow blast cells of 14 children with T cell acute lymphoblastic leukemia (T-ALL) using probes to the C mu and JH gene segments: At least one of the C mu-gene alleles was rearranged in three cases. In two of these, one C mu gene had the germ-line configuration and one was rearranged, whereas both alleles were rearranged in the third case. In one case, a rearranged heavy chain gene hybridized to the C mu-region probe, but not to the JH probe, indicating that the entire JH region had been deleted. These results demonstrate that immunoglobulin heavy chain gene rearrangements are not restricted to B lineage lymphoproliferative diseases in humans.

scholarly journals T-cell receptor delta gene rearrangement in childhood T-cell acute lymphoblastic leukemia

Blood ◽  
1989 ◽  
Vol 73 (8) ◽  
pp. 2133-2138
Author(s):  
A Biondi ◽  
E Champagne ◽  
V Rossi ◽  
G Giudici ◽  
A Cantu-Rajnoldi ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Gene Rearrangement ◽  
Lymphoblastic Leukemia ◽  
T Cell Development ◽  
Cell Development ◽  
Early Event ◽  
Chain Gene ◽  
Gamma Chain ◽  
Cell Acute Lymphoblastic Leukemia

During the development of functional T lymphocytes, a variety of genes involved in antigen recognition undergo somatic rearrangement. These include the alpha, beta, and gamma chain genes. Recently a fourth rearranging gene, the delta chain gene, embedded in the alpha chain locus, has been described. We have determined the structure of the beta, gamma, and delta chain genes in 15 cases of T-cell acute lymphoblastic leukemia (T-ALL) representing stage I (CD7+, CD1-, CD3-) and stage II (CD7+, CD1+, CD3-) of intrathymic T-cell development. The alpha-delta locus was rearranged in 14 of the 15 cases. In three cases the delta constant region was deleted on both chromosomes, suggesting biallelic V-J alpha rearrangement. A limited pattern of rearrangement of the delta locus was observed in the remaining 11 cases. When the alpha-delta region was rearranged, there was rearrangement of the beta and gamma TcR in all cases except two; in these cases the beta chain was in the germline configuration. These findings support the hypothesis that delta chain gene rearrangement is an early event in T- cell development, possibly contemporary to gamma gene rearrangement, and that the delta locus has a limited repertoire.

Presence of N regions in the clonotypic DJ rearrangements of the immunoglobulin heavy-chain genes indicates an exquisitely short latency in t(4;11)-positive infant acute lymphoblastic leukemia

Blood ◽  
2001 ◽  
Vol 98 (7) ◽  
pp. 2272-2274 ◽  
Author(s):  
Karin Fasching ◽  
Simon Panzer ◽  
Oskar A. Haas ◽  
Arndt Borkhardt ◽  
Rolf Marschalek ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Heavy Chain ◽  
Lymphoblastic Leukemia ◽  
Latency Period ◽  
Immunoglobulin Heavy Chain ◽  
In Utero ◽  
Cell Precursor ◽  
Developmentally Regulated ◽  
Igh Genes ◽  
Immunoglobulin Heavy Chain Genes

Childhood acute lymphoblastic leukemia (ALL) is frequently initiated in utero at a time of developmentally regulated insertion of N regions into the DJH rearrangements of immunoglobulin heavy-chain (IgH) genes. Here it is shown that N regions are present in the clonotypic DJH rearrangements in 11 of 12 infant ALLs with t(4;11). These data are compared with the 122 previously published DJH sequences and were found to have a pattern similar to that of ALL in children older than 3 years at diagnosis but were unlike that in children younger than 3 years who predominantly lack N regions. These findings, therefore, indicate that t(4;11)-positive infant ALL is initiated later in fetal development than most B-cell precursor ALL from children younger than 3 years and that they have a shorter latency period already in utero.

Sign in / Sign up

Export Citation Format

Share Document