scholarly journals Control of cell migration in the development of the posterior lateral line: antagonistic interactions between the chemokine receptors CXCR4 and CXCR7/RDC1

2007 ◽  
Vol 7 (1) ◽  
pp. 23 ◽  
Author(s):  
Christine Dambly-Chaudière ◽  
Nicolas Cubedo ◽  
Alain Ghysen
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Chemokine Receptors ◽  
Posterior Lateral Line ◽  
Antagonistic Interactions

scholarly journals Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260372
Author(s):  
Katherine S. Marsay ◽  
Sarah Greaves ◽  
Harsha Mahabaleshwar ◽  
Charmaine Min Ho ◽  
Henry Roehl ◽  
...  
Keyword(s):  
Cell Migration ◽  
Embryonic Development ◽  
Synergistic Effect ◽  
Lateral Line ◽  
Chemokine Receptors ◽  
Collective Cell Migration ◽  
Zebrafish Development ◽  
Posterior Lateral Line ◽  
Chemokine Signalling

Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate ligand, Cxcl12a, are essential for this process. We corroborate that knockdown of the zebrafish cd9 tetraspanin orthologue, cd9b, results in mild pLL abnormalities. Through generation of CRISPR and TALEN mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both Cd9a and Cd9b sensitized embryos to reduced Cxcr4b and Cxcl12a levels. Together these results provide evidence that Cd9 modulates collective cell migration of the pLLP during zebrafish development. One interpretation of these observations is that Cd9 contributes to more effective chemokine signalling.

scholarly journals CD9 tetraspanins convey robustness to CXCR4b signalling during collective cell migration

2021 ◽  
Author(s):  
Katherine S Marsay ◽  
Sarah Greaves ◽  
Henry Roehl ◽  
Peter N Monk ◽  
Thomas J Carney ◽  
...  
Keyword(s):  
Cell Migration ◽  
Embryonic Development ◽  
Lateral Line ◽  
Chemokine Receptors ◽  
Collective Cell Migration ◽  
Zebrafish Development ◽  
Posterior Lateral Line

Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, CXCR4b and CXCR7b, as well as their cognate ligand, CXCL12a, are essential for this process. Knockdown of the zebrafish CD9 tetraspanin orthologue, cd9b, displayed mild pLL abnormalities. Through generation of CRISPR mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both CD9a and CD9b sensitized embryos to reduced CXCR4b and CXCL12a levels. Together these results provide evidence that CD9 modulates collective cell migration of the pLLP through promoting CXCR4b signalling.

scholarly journals Mib1 contributes to persistent directional cell migration by regulating the Ctnnd1-Rac1 pathway

2017 ◽  
Vol 114 (44) ◽  
pp. E9280-E9289 ◽  
Author(s):  
Takamasa Mizoguchi ◽  
Shoko Ikeda ◽  
Saori Watanabe ◽  
Michiko Sugawara ◽  
Motoyuki Itoh
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Wound Closure ◽  
Cultured Cells ◽  
Small Gtpase ◽  
Precise Control ◽  
Animal Development ◽  
Posterior Lateral Line ◽  
Rac1 Activity ◽  
Directional Cell Migration

Persistent directional cell migration is involved in animal development and diseases. The small GTPase Rac1 is involved in F-actin and focal adhesion dynamics. Local Rac1 activity is required for persistent directional migration, whereas global, hyperactivated Rac1 enhances random cell migration. Therefore, precise control of Rac1 activity is important for proper directional cell migration. However, the molecular mechanism underlying the regulation of Rac1 activity in persistent directional cell migration is not fully understood. Here, we show that the ubiquitin ligase mind bomb 1 (Mib1) is involved in persistent directional cell migration. We found that knockdown of MIB1 led to an increase in random cell migration in HeLa cells in a wound-closure assay. Furthermore, we explored novel Mib1 substrates for cell migration and found that Mib1 ubiquitinates Ctnnd1. Mib1-mediated ubiquitination of Ctnnd1 K547 attenuated Rac1 activation in cultured cells. In addition, we found that posterior lateral line primordium cells in the zebrafish mib1ta52b mutant showed increased random migration and loss of directional F-actin–based protrusion formation. Knockdown of Ctnnd1 partially rescued posterior lateral line primordium cell migration defects in the mib1ta52b mutant. Taken together, our data suggest that Mib1 plays an important role in cell migration and that persistent directional cell migration is regulated, at least in part, by the Mib1–Ctnnd1–Rac1 pathway.

scholarly journals Histone Demethylase PHF8 Is Required for the Development of the Zebrafish Inner Ear and Posterior Lateral Line

2020 ◽  
Vol 8 ◽  
Author(s):  
Jing He ◽  
Zhiwei Zheng ◽  
Xianyang Luo ◽  
Yongjun Hong ◽  
Wenling Su ◽  
...  
Keyword(s):  
Cell Migration ◽  
Inner Ear ◽  
Lateral Line ◽  
Target Genes ◽  
Histone Demethylase ◽  
Otic Vesicle ◽  
Lateral Line System ◽  
Potential Candidate ◽  
Line System ◽  
Posterior Lateral Line

Histone demethylase PHF8 is crucial for multiple developmental processes, and hence, the awareness of its function in developing auditory organs needs to be increased. Using in situ hybridization (ISH) labeling, the mRNA expression of PHF8 in the zebrafish lateral line system and otic vesicle was monitored. The knockdown of PHF8 by morpholino significantly disrupted the development of the posterior lateral line system, which impacted cell migration and decreased the number of lateral line neuromasts. The knockdown of PHF8 also resulted in severe malformation of the semicircular canal and otoliths in terms of size, quantity, and position during the inner ear development. The loss of function of PHF8 also induced a defective differentiation in sensory hair cells in both lateral line neuromasts and the inner ear. ISH analysis of embryos that lacked PHF8 showed alterations in the expression of many target genes of several signaling pathways concerning cell migration and deposition, including the Wnt and FGF pathways. In summary, the current findings established PHF8 as a novel epigenetic element in developing auditory organs, rendering it a potential candidate for hearing loss therapy.

Cell migration in the postembryonic development of the fish lateral line

Development ◽  
2002 ◽  
Vol 129 (3) ◽  
pp. 605-615 ◽  
Author(s):  
Dora Sapède ◽  
Nicolas Gompel ◽  
Christine Dambly-Chaudière ◽  
Alain Ghysen
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Postembryonic Development ◽  
Cellular Level ◽  
Zebrafish Embryos ◽  
Two Dimensional ◽  
Posterior Lateral Line ◽  
Entire Structure ◽  
Astyanax Fasciatus ◽  
Blind Cavefish

We examine at the cellular level the postembryonic development of the posterior lateral line in the zebrafish. We show that the first wave of secondary neuromasts is laid down by a migrating primordium, primII. This primordium originates from a cephalic region much like the primordium that formed the primary line during embryogenesis. PrimII contributes to both the lateral and the dorsal branches of the posterior lateral line. Once they are deposited by the primordium, the differentiating neuromasts induce the specialisation of overlying epidermal cells into a pore-forming annulus, and the entire structure begins to migrate ventrally across the epithelium. Thus the final two-dimensional pattern depends on the combination of two orthogonal processes: anteroposterior waves of neuromast formation and dorsoventral migration of individual neuromasts. Finally, we examine how general these migratory processes can be by describing two fish species with very different adult patterns, Astyanax fasciatus (Mexican blind cavefish) and Oryzias latipes (medaka). We show that their primary patterns are nearly identical to that observed in zebrafish embryos, and that their postembryonic growth relies on the same combination of migratory processes that we documented in the case of the zebrafish.

scholarly journals Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Akihiro Urasaki ◽  
Seiya Morishita ◽  
Kosuke Naka ◽  
Minato Uozumi ◽  
Kouki Abe ◽  
...  
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Lateral Line System ◽  
Collective Cell Migration ◽  
Line System ◽  
Posterior Lateral Line
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