scholarly journals Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260372
Author(s):  
Katherine S. Marsay ◽  
Sarah Greaves ◽  
Harsha Mahabaleshwar ◽  
Charmaine Min Ho ◽  
Henry Roehl ◽  
...  
Keyword(s):  
Cell Migration ◽  
Embryonic Development ◽  
Synergistic Effect ◽  
Lateral Line ◽  
Chemokine Receptors ◽  
Collective Cell Migration ◽  
Zebrafish Development ◽  
Posterior Lateral Line ◽  
Chemokine Signalling

Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate ligand, Cxcl12a, are essential for this process. We corroborate that knockdown of the zebrafish cd9 tetraspanin orthologue, cd9b, results in mild pLL abnormalities. Through generation of CRISPR and TALEN mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both Cd9a and Cd9b sensitized embryos to reduced Cxcr4b and Cxcl12a levels. Together these results provide evidence that Cd9 modulates collective cell migration of the pLLP during zebrafish development. One interpretation of these observations is that Cd9 contributes to more effective chemokine signalling.

scholarly journals CD9 tetraspanins convey robustness to CXCR4b signalling during collective cell migration

2021 ◽  
Author(s):  
Katherine S Marsay ◽  
Sarah Greaves ◽  
Henry Roehl ◽  
Peter N Monk ◽  
Thomas J Carney ◽  
...  
Keyword(s):  
Cell Migration ◽  
Embryonic Development ◽  
Lateral Line ◽  
Chemokine Receptors ◽  
Collective Cell Migration ◽  
Zebrafish Development ◽  
Posterior Lateral Line

Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, CXCR4b and CXCR7b, as well as their cognate ligand, CXCL12a, are essential for this process. Knockdown of the zebrafish CD9 tetraspanin orthologue, cd9b, displayed mild pLL abnormalities. Through generation of CRISPR mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both CD9a and CD9b sensitized embryos to reduced CXCR4b and CXCL12a levels. Together these results provide evidence that CD9 modulates collective cell migration of the pLLP through promoting CXCR4b signalling.

scholarly journals Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Akihiro Urasaki ◽  
Seiya Morishita ◽  
Kosuke Naka ◽  
Minato Uozumi ◽  
Kouki Abe ◽  
...  
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Lateral Line System ◽  
Collective Cell Migration ◽  
Line System ◽  
Posterior Lateral Line

scholarly journals Eya1-dependent homeostasis of morphogenetic territories during collective cell migration

2021 ◽  
Author(s):  
Jerónimo R. Miranda-Rodríguez ◽  
Augusto Borges ◽  
Filipe Pinto-Teixeira ◽  
Indra Wibowo ◽  
Hans-Martin Pogoda ◽  
...  
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Chemokine Receptor ◽  
Collective Cell Migration ◽  
Intercellular Signaling ◽  
Collective Migration ◽  
Posterior Lateral Line ◽  
The Stability ◽  
Signaling Domains ◽  
Fgfr1 Expression

SUMMARYTissue remodeling presents an enormous challenge to the stability of intercellular signaling domains. Here we investigate this issue during the development of the posterior lateral line in zebrafish. We find that the transcriptional co-activator and phosphatase Eya1, mutated in the branchio-oto-renal syndrome in humans, is essential for the homeostasis of the Wnt/β-catenin and FGF morphogenetic domains during the collective migration of lateral-line primordial cells. Loss of Eya1 strongly diminishes the expression of Dkk1, expanding Wnt/β-catenin activity in the primordium, which in turn abrogates FGFR1 expression. Deficits in Eya1 also abolishes the expression of the chemokine receptor CXCR7b, disrupting primordium migration. These results reinforce the concept that morphogenetic domains in dynamically remodeling tissues are formed by cellular states maintained by continuous signaling.

scholarly journals Gβ1 controls collective cell migration by regulating the protrusive activity of leader cells in the posterior lateral line primordium

2014 ◽  
Vol 385 (2) ◽  
pp. 316-327 ◽  
Author(s):  
Hui Xu ◽  
Ding Ye ◽  
Martine Behra ◽  
Shawn Burgess ◽  
Songhai Chen ◽  
...  
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Collective Cell Migration ◽  
Posterior Lateral Line

scholarly journals Glypican4 modulates lateral line collective cell migration non cell-autonomously

2016 ◽  
Vol 419 (2) ◽  
pp. 321-335 ◽  
Author(s):  
Marina Venero Galanternik ◽  
Mark E. Lush ◽  
Tatjana Piotrowski
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Collective Cell Migration

scholarly journals Cell Proliferation and Collective Cell Migration During Zebrafish Lateral Line System Development Are Regulated by Ncam/Fgf-Receptor Interactions

2021 ◽  
Vol 8 ◽  
Author(s):  
Ramona Dries ◽  
Annemarie Lange ◽  
Sebastian Heiny ◽  
Katja I. Berghaus ◽  
Martin Bastmeyer ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Lateral Line ◽  
System Development ◽  
Lateral Line System ◽  
Collective Cell Migration ◽  
Fgf Receptor ◽  
Line System ◽  
Posterior Lateral Line ◽  
Neural Cell Adhesion ◽  
Reduce Cell Proliferation

The posterior lateral line system (pLLS) of aquatic animals comprises small clustered mechanosensory organs along the side of the animal. They develop from proneuromasts, which are deposited from a migratory primordium on its way to the tip of the tail. We here show, that the Neural Cell Adhesion Molecule Ncam1b is an integral part of the pathways initiating and regulating the development of the pLLS in zebrafish. We find that morpholino-knockdowns of ncam1b (i) reduce cell proliferation within the primordium, (ii) reduce the expression of Fgf target gene erm, (iii) severely affect proneuromast formation, and (iv) affect primordium migration. Ncam1b directly interacts with Fgf receptor Fgfr1a, and a knockdown of fgfr1a causes similar phenotypic changes as observed in ncam1b-morphants. We conclude that Ncam1b is involved in activating proliferation by triggering the expression of erm. In addition, we demonstrate that Ncam1b is required for the expression of chemokine receptor Cxcr7b, which is crucial for directed primordial migration. Finally, we show that the knockdown of ncam1b destabilizes proneuromasts, suggesting a further function of Ncam1b in strengthening the cohesion of proneuromast cells.

Sign in / Sign up

Export Citation Format

Share Document