scholarly journals Knockdown resistance (kdr)-like mutations in the voltage-gated sodium channel of a malaria vector Anopheles stephensi and PCR assays for their detection

2011 ◽  
Vol 10 (1) ◽  
Author(s):  
Om P Singh ◽  
Cherry L Dykes ◽  
Manila Lather ◽  
Om P Agrawal ◽  
Tridibes Adak
Keyword(s):  
Sodium Channel ◽  
Malaria Vector ◽  
Anopheles Stephensi ◽  
Knockdown Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Pcr Assays

scholarly journals A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Raja Babu S. Kushwah ◽  
Taranjeet Kaur ◽  
Cherry L. Dykes ◽  
H. Ravi Kumar ◽  
Neera Kapoor ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Kdr Mutation ◽  
Knockdown Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated

scholarly journals The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wan Fatma Zuharah ◽  
Maryam Sufian
Keyword(s):  
Sodium Channel ◽  
Vector Control ◽  
Novel Mutation ◽  
Control Program ◽  
Knockdown Resistance ◽  
Genotypic Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Vector Control Program ◽  
Current Resistance

AbstractThe usage of insecticide rendered the successful vector control program with the high usage of the pyrethroid. However, the intensive and extensive use of pyrethroid, causing resistance in Aedesaegypti and hampered the control program. Knockdown resistance (kdr) resulting from the Voltage-Gated Sodium Channel (VGSC) is one of the mechanisms of resistance in pyrethroid group insecticide. Investigating the phenotypic status of Ae.aegypti mosquitoes is a lead in knowing the current resistance status and as an indicator of the genotypic resistance. In this study, we investigate the resistance in phenotypic and genotypic of Ae.aegypti with a new kdr mutation point A1007G was detected. Using the adult bioassay, we tested the phenotypic resistance from the Selangor state against 0.75% permethrin, 0.05% deltamethrin with and without the addition of PBO synergist. Permethrin-resistant and deltamethrin-resistant, including susceptible samples, were subjected to genotyping analysis on mutation point in domain II and domain III of Voltage-Gated Sodium Channel (VGSC). Adult bioassay revealed that the Ae.aegypti was highly resistance toward 0.75% permethrin and 0.05% deltamethrin. The bioassay with the presence of PBO synergist showed an increment of mortality rate, but Ae.aegypti status is still resistance towards both insecticides. Genotyping result showed that three common kdr mutations (S989P, V1016G, and F1534C) have existed in the Ae.aegypti population. A new novel mutation on A1007G was also detected in this population, which is the first time reported. This study has brought a piece of information on the current resistance status in Ae.aegypti in Malaysia. The detection of new mutation point of A1007G has added the knowledge on the resistance in mosquitoes. Thus, this study will aid with the decision making in the usage of insecticides in the vector control program; before this invaluable insecticide rendered ineffective in killing mosquitoes.

scholarly journals The discovery of a novel knockdown resistance (kdr) mutation A1007G in the Voltage-Gated Sodium Channel (vgsc) on Aedes aegypti (Diptera: Culicidae) from Malaysia

2020 ◽  
Author(s):  
Wan Fatma Zuharah ◽  
Maryam Sufian
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Control Program ◽  
Kdr Mutation ◽  
Knockdown Resistance ◽  
New Mutation ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Vector Control Program ◽  
Current Resistance

Abstract Background: The usage of insecticide rendered the successful vector control program with the high usage of the pyrethroid. However, the intensive and extensive use of pyrethroid, causing resistance in Aedes aegypti and hampered the control program. Knockdown resistance (kdr) resulting from the Voltage-Gated Sodium Channel (vgsc) is one of the mechanisms of resistance in pyrethroid group insecticide. Investigating the phenotypic status of Ae. aegypti mosquitoes is a lead in knowing the current resistance status and as an indicator of the genotypic resistance. In this study, we investigate the resistance in phenotypic and genotypic of Ae. aegypti with a new kdr mutation point, A1007G was detected. Methods: Using the adult bioassay, we tested the phenotypic resistance from the Selangor state against 0.75% permethrin, 0.05% deltamethrin with and without the addition of PBO synergist. Permethrin-resistant and deltamethrin-resistant, including susceptible samples, were subjected to genotyping analysis on mutation point in domain II and domain III of Voltage-Gated Sodium Channel (vgsc). Results: Adult bioassay revealed that the Ae. aegypti was highly resistance toward 0.75% permethrin and 0.05% deltamethrin. The bioassay with the presence of PBO synergist showed an increment of mortality rate, but the status of Ae. aegypti is still resistance towards both insecticides. Genotyping result showed that three common kdr mutations (S989P, V1016G, and F1534C) have existed in the Ae. aegypti population. Luckily, a new mutation on A1007G was also detected in this population, and this is the first time reported. Conclusions: This study has brought a piece of information on the current resistance status in Ae. aegypti in Malaysia. The detection of new mutation point of A1007G has added the knowledge on the resistance in mosquitoes. Thus, this study will aid the decision in using the insecticide usage in vector control program before this invaluable insecticide rendered ineffective in killing mosquitoes.

scholarly journals A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G

2019 ◽  
Author(s):  
Raja Babu S. Kushwah ◽  
Taranjeet Kaur ◽  
Cherry L. Dykes ◽  
Ravi H. Kumar ◽  
Neera Kapoor ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dna Sequencing ◽  
Insecticide Resistance ◽  
Sodium Channel ◽  
Vector Control ◽  
Knockdown Resistance ◽  
New Mutation ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Domain Iii

AbstractBackgroundAedes aegypti is a primary vector of dengue, chikungunya and zika infections in India. In the absence of specific drugs or safe and effective vaccines for these infections, their control relies mainly on vector control measures. The emergence of insecticide resistance in vectors, especially against pyrethroids, is a serious threat to the insecticide-based vector control programme. This study reports the presence of multiple knockdown resistance (kdr) mutations present in an Ae. aegypti population from Bengaluru (India), including a new mutation F1534L.MethodsAedes aegypti collected from Bengaluru were subjected to insecticide susceptibility tests with DDT, deltamethrin and permethrin. The DNA sequencing of partial domain II, III and IV of the voltage-gated sodium channel (VGSC) was performed to screen kdr mutations present in the population and PCR-based assays were developed for their detection. Genotyping of kdr mutations was done using PCR-based assays, allelic frequencies were determined, and tests of genetic association of kdr mutations with the insecticide resistance phenotype were performed.ResultsThe Ae. aegypti population were resistant to DDT, deltamethrin and permethrin. The DNA sequencing of the VGSC revealed the presence of four kdr mutations, i.e., S989P and V1016G in domain II and two alternative kdr mutations F1534C and F1534L in domain III. Allele-specific PCR assays (ASPCR) were developed for the detection of kdr mutations S989P and V1016G and an existing PCR-RFLP based strategy was modified for the genotyping of all three known kdr mutations in domain III (F1534L, F1534C and T1520I). Genotyping of Ae. aegypti samples collected between October 2014 and April 2015 revealed a moderate frequency of S989P/V1016G (18.27%) and F1534L (17.48%), a relatively high frequency of F1534C (50.61%) and absence of T1520I in the population. Mutations S989P and V1016G were in complete linkage disequilibrium in this population while they were in linkage equilibrium with kdr mutations F1534C and F1534L. The alleles F1534C and F1534L are genetically associated with permethrin resistance.ConclusionsA new kdr mutation, F1534L, was found in an Ae. aegypti population from Bengaluru (India), co-occurring with the other three mutations S989P, V1016G and F1534C. The findings of a new mutation and development of PCR-based diagnostics have implications for insecticide resistance management. Monitoring of F1534L-kdr in other populations and studies on their functional role in altering neuro-sensitivity is warranted.

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