scholarly journals Inhibition of hepatitis C virus by an M1GS ribozyme derived from the catalytic RNA subunit of Escherichia coli RNase P

2014 ◽  
Vol 11 (1) ◽  
pp. 86 ◽  
Author(s):  
Xinliang Mao ◽  
Xifang Li ◽  
Xinjun Mao ◽  
Zhiwen Huang ◽  
Chengcheng Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rnase P ◽  
Catalytic Rna

Immunological evaluation of Escherichia coli -derived hepatitis C virus second envelope protein (E2) variants

2001 ◽  
Vol 58 (3) ◽  
pp. 221-228 ◽  
Author(s):  
S. Dueñas-Carrera ◽  
A. Viña ◽  
H.E. Garay ◽  
O. Reyes ◽  
L. Alvarez-Lajonchere ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Protein ◽  
Immunological Evaluation

Canola oilseed‐ and Escherichia coli‐ derived hepatitis C virus (HCV) core proteins adjuvanted with oil bodies, induced robust Th1‐oriented immune responses in immunized mice

Apmis ◽  
2020 ◽  
Vol 128 (11) ◽  
pp. 593-602
Author(s):  
Sara Mohammadzadeh ◽  
Farzin Roohvand ◽  
Parastoo Ehsani ◽  
Ali Hatef Salmanian ◽  
Soheila Ajdary
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immune Responses ◽  
Oil Bodies ◽  
Core Proteins

scholarly journals Geneticin Stabilizes the Open Conformation of the 5′ Region of Hepatitis C Virus RNA and Inhibits Viral Replication

2015 ◽  
Vol 60 (2) ◽  
pp. 925-935 ◽  
Author(s):  
Ascensión Ariza-Mateos ◽  
Rosa Díaz-Toledano ◽  
Timothy M. Block ◽  
Samuel Prieto-Vega ◽  
Alex Birk ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Therapeutic Potential ◽  
Kinetic Studies ◽  
Rnase P ◽  
Start Codon ◽  
Hcv Rna ◽  
Rnase Iii ◽  
Entry Site ◽  
Content Type

ABSTRACTThe aminoglycoside Geneticin (G418) is known to inhibit cell culture proliferation, via virus-specific mechanisms, of two different virus genera from the familyFlaviviridae. Here, we tried to determine whether Geneticin can selectively alter the switching of the nucleotide 1 to 570 RNA region of hepatitis C virus (HCV) and, if so, whether this inhibits viral growth. Two structure-dependent RNases known to specifically cleave HCV RNA were tested in the presence or absence of the drug. One was theSynechocystissp. RNase P ribozyme, which cleaves the tRNA-like domain around the AUG start codon under high-salt buffer conditions; the second wasEscherichia coliRNase III, which recognizes a double-helical RNA switch element that changes the internal ribosome entry site (IRES) from a closed (C) conformation to an open (O) one. While the drug did not affect RNase P activity, it did inhibit RNase III in the micromolar range. Kinetic studies indicated that the drug favors the switch from the C to the O conformation of the IRES by stabilizing the distal double-stranded element and inhibiting further processing of the O form. We demonstrate that, because the RNA in this region is highly conserved and essential for virus survival, Geneticin inhibits HCV Jc1 NS3 expression, the release of the viral genomic RNA, and the propagation of HCV in Huh 7.5 cells. Our study highlights the crucial role of riboswitches in HCV replication and suggests the therapeutic potential of viral-RNA-targeted antivirals.

Purification and characterization of hepatitis C virus non-structural protein 5A expressed in Escherichia coli

2004 ◽  
Vol 37 (1) ◽  
pp. 144-153 ◽  
Author(s):  
Luyun Huang ◽  
Elena V Sineva ◽  
Michele R.S Hargittai ◽  
Suresh D Sharma ◽  
Mehul Suthar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Structural Protein ◽  
Purification And Characterization

scholarly journals Expression of alternate reading frame protein (F1) of hepatitis C virus in Escherichia coli and detection of antibodies for F1 in Indian patients

2008 ◽  
Vol 8 (3) ◽  
pp. 374-377 ◽  
Author(s):  
Munpally Shesheer Kumar ◽  
Khareedu Venkateswara Rao ◽  
Chittor Mohammed Habeebullah ◽  
Vudem Dashavantha Reddy
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Reading Frame ◽  
Alternate Reading Frame Protein ◽  
Indian Patients

Expression and immunological evaluation of the Escherichia coli-derived hepatitis C virus envelope E1 protein

2000 ◽  
Vol 32 (2) ◽  
pp. 137 ◽  
Author(s):  
Lázaro J. Lorenzo ◽  
Odalys García ◽  
Nelson Acosta-Rivero ◽  
Santiago Dueñas-Carrera ◽  
Gillian Martínez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Envelope ◽  
E1 Protein ◽  
Immunological Evaluation

scholarly journals Multiple binding modes of substrate to the catalytic RNA subunit of RNase P from Escherichia coli

RNA ◽  
1999 ◽  
Vol 5 (8) ◽  
pp. 1021-1033 ◽  
Author(s):  
DANIEL A. POMERANZ KRUMMEL ◽  
SIDNEY ALTMAN
Keyword(s):  
Escherichia Coli ◽  
Rnase P ◽  
Catalytic Rna ◽  
Binding Modes ◽  
Multiple Binding
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