scholarly journals Plant sequence completed

2000 ◽  
Vol 1 ◽  
pp. spotlight-20001214-02
Author(s):  
William Wells
Keyword(s):  
Plant Sequence

scholarly journals Type IV Effector Proteins Involved in the Medicago-Sinorhizobium Symbiosis

2017 ◽  
Vol 30 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Matthew S. Nelson ◽  
Chan Lan Chun ◽  
Michael J. Sadowsky
Keyword(s):  
Transcriptional Activation ◽  
Sinorhizobium Meliloti ◽  
Effector Proteins ◽  
Nodule Formation ◽  
Effector Protein ◽  
In Planta ◽  
Type Iv ◽  
Rhizobial Species ◽  
Polymerase Chain ◽  
Plant Sequence

In this study, we investigated genetic elements of the type IV secretion system (T4SS) found in Sinorhizobium spp. and the role they play in symbiosis. Sinorhizobium meliloti and S. medicae each contain a putative T4SS similar to that used by Agrobacterium tumefaciens during pathogenesis. The Cre reporter assay for translocation system was used to validate potential effector proteins. Both S. meliloti and S. medicae contained the effector protein TfeA, which was translocated into the host plant. Sequence analysis revealed the presence of a nod box involved in transcriptional activation of symbiosis-related genes, upstream of the transcriptional regulator (virG) in the Sinorhizobium T4SS. Replicate quantitative reverse transcription-polymerase chain reaction analyses indicated that luteolin, released by roots and seeds of Medicago truncatula, upregulated transcription of tfeA and virG. Mutations in the T4SS apparatus or tfeA alone resulted in reduced numbers of nodules formed on M. truncatula genotypes. In addition, S. meliloti KH46c, which contains a deletion in the T4SS, was less competitive for nodule formation when coinoculated with an equal number of cells of the wild-type strain. To our knowledge, TfeA is the first T4SS effector protein identified in Sinorhizobium spp. Our results indicate that Sinorhizobium i) uses a T4SS during initiation of symbiosis with Medicago spp., and ii) alters Medicago cells in planta during symbiosis. This study also offers additional bioinformatic evidence that several different rhizobial species may use the T4SS in symbiosis with other legumes.

scholarly journals Mercator: a fast and simple web server for genome scale functional annotation of plant sequence data

2013 ◽  
Vol 37 (5) ◽  
pp. 1250-1258 ◽  
Author(s):  
MARC LOHSE ◽  
AXEL NAGEL ◽  
THOMAS HERTER ◽  
PATRICK MAY ◽  
MICHAEL SCHRODA ◽  
...  
Keyword(s):  
Functional Annotation ◽  
Sequence Data ◽  
Web Server ◽  
Genome Scale ◽  
Plant Sequence

Plant Sequence Capture Optimised for Illumina Sequencing

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (13) ◽  
Author(s):  
Axel Himmelbach ◽  
Manuela Knauft ◽  
Nils Stein
Keyword(s):  
Illumina Sequencing ◽  
Sequence Capture ◽  
Plant Sequence

scholarly journals Logic Verification System for Power Plant Sequence Diagrams

1994 ◽  
Vol 31 (2) ◽  
pp. 102-112 ◽  
Author(s):  
Mitsuko FUKUDA ◽  
Naoyuki YAMADA ◽  
Toshiaki TESHIMA ◽  
Ken'ichi KAN ◽  
Mitsugu UTSUNOMIYA
Keyword(s):  
Power Plant ◽  
Sequence Diagrams ◽  
Verification System ◽  
Logic Verification ◽  
Plant Sequence

scholarly journals A novel distinct genetic variant of tomato torrado virus with substantially shorter RNA1-specific 3'untranslated region (3'UTR)

2021 ◽  
Author(s):  
Marta Budziszewska ◽  
Przemysław Wieczorek ◽  
Aleksandra Obrępalska-Stęplowska
Keyword(s):  
Amino Acid ◽  
Untranslated Region ◽  
Genetic Variant ◽  
Sequencing Data ◽  
Sequence Analyses ◽  
Nucleotide Substitutions ◽  
Systemic Necrosis ◽  
Single Nucleotide ◽  
Plant Sequence

Abstract Tomato torrado virus (ToTV) induces severe systemic necrosis in Solanum lycopersicum. This work aimed at describing the genetic variability of necrosis-inducing ToTV-Wal'17 collected in the 2017 year, derived from the ToTV-Wal'03 after long-term passages in plant. Sequence analyses of the ToTV-Wal`17 indicated twenty-eight single nucleotide substitutions in coding sequence of both RNAs, twelve of which resulted in amino acid changes in viral polyproteins. Moreover the sequencing data revealed that the 3'UTR of ToTV-Wal'17 RNA1 was 394 nts shorter in comparison to Wal'03. The performed sequence analyses pointed that 3'UTR of RNA1of ToTV-Wal'17 is the most divergent across all previously described European isolates.

scholarly journals Amplification rate of matK and rbcL genes in three types of durian

2021 ◽  
Vol 886 (1) ◽  
pp. 012011
Author(s):  
Y F Cahyaningsih ◽  
Sukartini ◽  
A Sutanto ◽  
P J Santoso ◽  
M A Arsyad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Plant Species ◽  
Genetic Relationship ◽  
Genetic Relationships ◽  
Chloroplast Genes ◽  
Rbcl Sequence ◽  
Durio Zibethinus ◽  
Amplification Rate ◽  
Plant Sequence

Abstract Durian is a tropic fruit having numerous variations on its fruits. Its variations are not only in its shape but also in its aril fruit, aril color, flavor, and aril thickness. In addition to its fruit variations, the genus Durio also has many species which quite hard to distinguish morphologically, except during flowering and fruiting times. This study aimed to determine the genetic relationship among Durian, Pelangi Atuturi Variety Durian, Durio graveolent, and Durio zibetinus based on chloroplast genes (RbcL and matK genes). The primers were previously designed for amplifying matK and rbcL genes based on the Durio zibethinus sequence. Both genes were used because of having great competence to describe genetic relationships between plant species. The rbcL primer could amplify all evaluated samples. Meanwhile, matK primer generated a smeared band in Durian Pelangi; thus, we did not obtain any sequence of this plant. Sequence analysis showed no variation of rbcL sequence in these evaluated species. A similar result was also observed on D. zibethinus and D. graveolent. Overall, both genes could not describe the genetic relationship among the evaluated durians, and they were grouped in the same cluster in phylogenetic.

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