scholarly journals Reverse Genetics Assembly of Newcastle Disease Virus Genome Template Using Asis-Sal-Pac BioBrick Strategy

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Amin Tavassoli ◽  
Safoura Soleymani ◽  
Alireza Haghparast ◽  
Gholamreza Hashemi Tabar ◽  
Mohammad Reza Bassami ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Virus Genome

Use of Reverse Genetics to Enhance the Oncolytic Properties of Newcastle Disease Virus

2007 ◽  
Vol 67 (17) ◽  
pp. 8285-8292 ◽  
Author(s):  
Adam Vigil ◽  
Man-Seong Park ◽  
Osvaldo Martinez ◽  
Mark A. Chua ◽  
Sa Xiao ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics

scholarly journals An improved method for the rescue of recombinant Newcastle disease virus

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 96-100
Author(s):  
Pheik-Sheen Cheow ◽  
Tiong Kit Tan ◽  
Adelene Ai-Lian Song ◽  
Khatijah Yusoff ◽  
Suet Lin Chia
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Vaccine Development ◽  
Transfection Efficiency ◽  
Transfection Method ◽  
Helper Plasmids ◽  
Immunogenic Properties ◽  
Recombinant Newcastle Disease Virus

Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method.

Rescue of recombinant Newcastle disease virus: a promising vector with two decades of intensive vaccine research

2019 ◽  
Vol 14 (9) ◽  
pp. 617-628 ◽  
Author(s):  
Aidin Molouki ◽  
Abdou Nagy
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Progeny Virus ◽  
Vaccine Research ◽  
Human Use ◽  
Helper Plasmids ◽  
Initiation Sequence ◽  
Recombinant Newcastle Disease Virus

Two decades have passed since the first reverse genetics system for the rescue of recombinant Newcastle disease virus was developed. Since then, the recombinant Newcastle disease virus vector has shown promising results as a safe and potent vector for development of many vaccines for both avian and human use. Herein, we review several technical topics that would be useful to further understanding of this technology. First, the effect of using helper plasmids encoding proteins belonging to strains other than the full-length cDNA and the possible incorporation of these expressed proteins into progeny virus will be discussed. Then, we will discuss the effect of removal of additional G residues from the T7 initiation sequence and finally, we will review different ways to improve rescue efficiency.

RNA sequence and transcriptional properties of the 3′ end of the Newcastle disease virus genome

Virology ◽  
1985 ◽  
Vol 145 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Michael G. Kurilla ◽  
Henry O. Stone ◽  
Jack D. Keene
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Genome ◽  
Rna Sequence

scholarly journals The Hemagglutinin-Neuraminidase Protein of Newcastle Disease Virus Determines Tropism and Virulence

2004 ◽  
Vol 78 (8) ◽  
pp. 4176-4184 ◽  
Author(s):  
Zhuhui Huang ◽  
Aruna Panda ◽  
Subbiah Elankumaran ◽  
Dhanasekaran Govindarajan ◽  
Daniel D. Rockemann ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Index Test ◽  
Death Time ◽  
Virulent Virus ◽  
Hn Gene ◽  
Chimeric Viruses

ABSTRACT The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in the process of infection. However, the exact contribution of the HN gene to NDV pathogenesis is not known. In this study, the role of the HN gene in NDV virulence was examined. By use of reverse genetics procedures, the HN genes of a virulent recombinant NDV strain, rBeaudette C (rBC), and an avirulent recombinant NDV strain, rLaSota, were exchanged. The hemadsorption and neuraminidase activities of the chimeric viruses showed significant differences from those of their parental strains, but heterotypic F and HN pairs were equally effective in fusion promotion. The tissue tropism of the viruses was shown to be dependent on the origin of the HN protein. The chimeric virus with the HN protein derived from the virulent virus exhibited a tissue predilection similar to that of the virulent virus, and vice versa. The chimeric viruses with reciprocal HN proteins either gained or lost virulence, as determined by a standard intracerebral pathogenicity index test of chickens and by the mean death time in chicken embryos (a measure devised to classify these viruses), indicating that virulence is a function of the amino acid differences in the HN protein. These results are consistent with the hypothesis that the virulence of NDV is multigenic and that the cleavability of F protein alone does not determine the virulence of a strain.

scholarly journals Author response: Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome

2019 ◽  
Author(s):  
Xiyong Song ◽  
Hong Shan ◽  
Yanping Zhu ◽  
Shunlin Hu ◽  
Ling Xue ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Genome ◽  
Author Response

Sequence variation in the Newcastle disease virus genome

2006 ◽  
Vol 116 (1-2) ◽  
pp. 168-184 ◽  
Author(s):  
Jacqueline A. Kattenbelt ◽  
Matthew P. Stevens ◽  
Allan R. Gould
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Sequence Variation ◽  
Virus Genome

scholarly journals Phosphoprotein Contributes to the Thermostability of Newcastle Disease Virus

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yang Zhao ◽  
Huairan Liu ◽  
Feng Cong ◽  
Wei Wu ◽  
Ran Zhao ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Major Threat ◽  
P Protein ◽  
Growth Properties ◽  
Similar Growth ◽  
Thermostable Vaccine ◽  
Chimeric Viruses

Newcastle disease (ND), caused by Newcastle disease virus (NDV), is highly contagious and represents a major threat to the poultry industry. The thermostable vaccines are not insensitive to heat and ease of storage and transportation, but the mechanism of NDV thermostability remains unknown. The phosphoprotein (P), fusion protein (F), hemagglutinin-neuraminidase protein (HN), and large polymerase protein (L) are associated with NDV virulence. The association between F, HN, or L and viral thermostability has been, respectively, studied in different reports. However, the effects of P on NDV thermostability have not been demonstrated. Here, we utilized an existing reverse genetics system in our laboratory, to generate chimeric viruses by exchanging the P protein between the thermostable NDV4-C strain and the thermolabile LaSota strain. Chimeric viruses were found to possess similar growth properties, passage stability, and virulence, as compared to those of these parental strains. Interestingly, the thermostability of the chimera with P derived from the thermolabile LaSota strain was reduced compared to that of the parental virus, and P of the thermostable NDV4-C strain enhanced chimeric virus thermostability. Our data demonstrate that P is an important factor for the thermostability of NDV and provides information regarding the molecular mechanism of NDV thermostability; moreover, these results suggest a theoretical basis for using the NDV4-C strain as a thermostable vaccine.

Sign in / Sign up

Export Citation Format

Share Document