scholarly journals Molecular cloning and characterization of a grapevine (Vitis vinifera L.) serotonin N-acetyltransferase (VvSNAT2) gene involved in plant defense

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Yihe Yu ◽  
Lu Bian ◽  
Zeling Jiao ◽  
Keke Yu ◽  
Yutong Wan ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Vitis Vinifera ◽  
Plant Defense ◽  
Marker Genes ◽  
Vitis Vinifera L ◽  
Melatonin Treatment ◽  
Gene Encoding ◽  
Ja Signaling ◽  
First Time

Abstract Background Melatonin is a ubiquitous molecule and exists across kingdoms. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. A number of studies have been conducted on the melatonin content and exogenous melatonin treatment of grapevine (Vitis vinifera L.). However, key genes or enzymes of the melatonin biosynthetic pathway remain unclear. Results In this study, we cloned and identified the gene encoding serotonin N-acetyltransferase (SNAT) in grapevine (VvSNAT2). The VvSNAT2 protein was identified from a collection of 30 members of the grapevine GCN5-related N-acetyltransferase (GNAT) superfamily. Phylogenetic and protein sublocalization analyses showed that the candidate gene VvGNAT16 is VvSNAT2. Characterization of VvSNAT2 showed that its enzymatic activity is highest at a pH of 8.8 and a temperature of 45 °C. Analysis of enzyme kinetics showed the values of Km and Vmax of VvSNAT2 using serotonin were 392.5 μM and 836 pmol/min/mg protein, respectively. The expression of VvSNAT2 was induced by melatonin treatment and pathogen inoculation. Overexpression of VvSNAT2 in Arabidopsis resulted in greater accumulation of melatonin and chlorophyll and enhanced resistance to powdery mildew in the transgenic plants compared with the wild type (WT). Additionally, our data showed that the marker genes in the salicylic acid (SA) signaling pathway were expressed to higher levels in the transgenic plants compared with the WT. Conclusions The VvSNAT2 gene was cloned and identified in grapevine for the first time. Our results indicate that VvSNAT2 overexpression activates the SA and JA signaling pathways; however, the SA pathway plays a central role in VvSNAT2-mediated plant defense.

scholarly journals Direct Identification and Characterization of Phenolic Compounds from Crude Extracts of Buds and Internodes of Grapevine (Vitis vinifera cv Merlot)

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901
Author(s):  
Said Qsaib ◽  
Nuno Mateus ◽  
Fatima Ez-zohra Ikbal ◽  
Lala Aicha Rifai ◽  
Victor de Freitas ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Reverse Phase Hplc ◽  
Direct Identification ◽  
Array Detection ◽  
First Time ◽  
Identification And Characterization

The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and ε-viniferin had similar levels in buds and internodes while six other compounds identified had higher levels in buds.

scholarly journals Molecular Characterization of the Gene Encoding a New AmpC β-Lactamase in a Clinical Strain of Acinetobacter Genomic Species 3

2004 ◽  
Vol 48 (4) ◽  
pp. 1374-1378 ◽  
Author(s):  
Alejandro Beceiro ◽  
Lourdes Dominguez ◽  
Anna Ribera ◽  
Jordi Vila ◽  
Francisca Molina ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Biochemical Properties ◽  
Clinical Strain ◽  
The Novel ◽  
Gene Encoding ◽  
Genomic Species ◽  
First Time

ABSTRACT A presumptive chromosomal cephalosporinase (pI, 9.0) from a clinical strain of Acinetobacter genomic species 3 (AG3) is reported. The nucleotide sequence of this β-lactamase shows for the first time the gene encoding an AmpC enzyme in AG3. In addition, the biochemical properties of the novel AG3 AmpC β-lactamase are reported

scholarly journals Characterization of endophyt fungi in bali grapevine (vitis vinifera l. var alphonso lavalle) in Buleleng, Bali

2019 ◽  
Vol 1317 ◽  
pp. 012083
Author(s):  
Ni Putu Ristiati ◽  
Ida Ayu Putu Suryanti ◽  
Ni Luh Putu Manik Widiyanti ◽  
AAIA Rai Sudiatmika ◽  
Anggan Pradipta Utama
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L

scholarly journals Preliminary Characterization of Wild Grapevine Populations (Vitis vinifera ssp. sylvestris) Grown Along the Danube River

2013 ◽  
Vol 41 (2) ◽  
pp. 472 ◽  
Author(s):  
Carmen F. POPESCU ◽  
Liviu C. DEJEU ◽  
Rafael R. OCETE
Keyword(s):  
Vitis Vinifera ◽  
Morphological Analysis ◽  
Danube River ◽  
Vitis Vinifera L ◽  
Meristematic Tissue ◽  
The Danube River ◽  
Wild Grapevine ◽  
The Moment

The individuals belonging to three different groups of wild grapevines populations Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi harvested along, or near the Danube River, were described by means of usual ampelographic methods. The twenty standardized descriptors used for morphological analysis revealed obvious differentiation among analyzed populations. Out of 65 individuals, a half produced flowers with separate sex and a high proportion of them were males (70%). Pollen measurements on light microscope provided information on differences in pollen size among inside wild grapevine populations of V. sylvestris with the polar length varying between 15.3 and 23 μm and the equatorial length between 15.5 and 24.4μm. The in vitro regenerative potential from meristematic tissue tested with each phenotype showed that the moment of differentiation, the aspect of proliferative structures and the rate of multiplication varied inside these wild grapevine populations, without any correlation with the location of harvesting. Our results provided valuable information about these Vitis vinifera ssp. sylvestris populations, possible to be used as starting plant material for research in general and further breeding of cultivars and grapevine rootstocks.

Characterization of phenolic composition in Carignan noir grapes (Vitis vinifera L.) from six wine-growing sites in Maule Valley, Chile

2017 ◽  
Vol 98 (1) ◽  
pp. 274-282 ◽  
Author(s):  
Ana M Martínez-Gil ◽  
Gastón Gutiérrez-Gamboa ◽  
Teresa Garde-Cerdán ◽  
Eva P Pérez-Álvarez ◽  
Yerko Moreno-Simunovic
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Phenolic Composition

scholarly journals Genome-wide identification and characterization of the metal tolerance protein (MTP) family in grape (Vitis vinifera L.)

3 Biotech ◽  
2019 ◽  
Vol 9 (5) ◽  
Author(s):  
Zahra Shirazi ◽  
Amin Abedi ◽  
Mojtaba Kordrostami ◽  
David J. Burritt ◽  
Mohammad Anwar Hossain
Keyword(s):  
Vitis Vinifera ◽  
Metal Tolerance ◽  
Vitis Vinifera L ◽  
Genome Wide ◽  
Metal Tolerance Protein ◽  
Identification And Characterization

Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

2014 ◽  
Vol 9 (8) ◽  
pp. 761-767
Author(s):  
Ivana Dokupilová ◽  
Daniele Migliaro ◽  
Daniel Mihálik ◽  
Manna Crespan ◽  
Ján Kraic
Keyword(s):  
Genetic Diversity ◽  
Vitis Vinifera ◽  
Genetic Resources ◽  
Genetic Variants ◽  
Genetic Resource ◽  
Vitis Vinifera L ◽  
Total Genetic Diversity ◽  
Unusual Phenomenon ◽  
Resource Collection

AbstractMicrosatellites were used as a very effective tool for genetic diversity analysis and characterization of 51 grapevine (Vitis vinifera L.) accessions from the national collection of genetic resources. Genetic diversity was relatively high, 8.91 alleles were detected per analysed microsatellite locus in average, and fifty-one accessions were distinguished into 45 groups. Distribution of recent Slovak cultivars across the dendrogram accented both their genetic diversity and the effectiveness of the national breeding program in maintaining genetic diversity and generating new genetic variants. Each cultivar was different from the others and twelve of them contained 77.6% of the total genetic diversity of the whole analysed set. Microsatellite patterns were also able to confirm parentage in selected Slovak cultivars. An unusual phenomenon of triallelism was also detected in one of the analysed accessions. The present study has initiated molecular characterization within the national grapevine genetic resource collection and their comparison with well-established international cultivars.

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