scholarly journals Melanoma cells replicate through chemotherapy by reducing levels of key homologous recombination protein RAD51 and increasing expression of translesion synthesis DNA polymerase ζ

BMC Cancer ◽  
2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Liang Song ◽  
Ewan M. McNeil ◽  
Ann-Marie Ritchie ◽  
Katy R. Astell ◽  
Charlie Gourley ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Melanoma Cells ◽  
Recombination Protein

scholarly journals Role of Translesion Synthesis DNA Polymerases in DNA Replication in the Presence of a Weak DNA Polymerase δ in Saccharomyces cerevisiae

2018 ◽  
Vol 8 (2) ◽  
pp. 754-754
Author(s):  
Likui Zhang ◽  
Yanchao Huang ◽  
Xinyuan Zhu ◽  
Yuxiao Wang ◽  
Haoqiang Shi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Dna Polymerase Δ

scholarly journals Replication Past the -Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Paromita Raychaudhury ◽  
Ashis K. Basu
Keyword(s):  
Dna Polymerase ◽  
Mammalian Cells ◽  
Translesion Synthesis ◽  
Kidney Cells ◽  
Equal Frequency ◽  
Cross Link ◽  
Dominant Mutation ◽  
Human Embryonic Kidney Cells ◽  
Radiation Induced

-Radiation-induced intrastrand guanine-thymine cross-link, G[8,5-Me]T, hinders replicationin vitroand is mutagenic in mammalian cells. Herein we reportin vitrotranslesion synthesis of G[8,5-Me]T by human and yeast DNA polymerase (hPol and yPol ). dAMP misincorporation opposite the cross-linked G by yPol was preferred over correct incorporation of dCMP, but further extension was 100-fold less efficient for :A compared to :C. For hPol , both incorporation and extension were more efficient with the correct nucleotides. To evaluate translesion synthesis in the presence of all four dNTPs, we have developed a plasmid-based DNA sequencing assay, which showed that yPol was more error-prone. Mutational frequencies of yPol and hPol were 36% and 14%, respectively. Targeted was the dominant mutation by both DNA polymerases. But yPol induced targeted in 23% frequency relative to 4% by hPol . For yPol , targeted and constituted 83% of the mutations. By contrast, with hPol , semi-targeted mutations (7.2%), that is, mutations at bases near the lesion, occurred at equal frequency as the targeted mutations (6.9%). The kind of mutations detected with hPol showed significant similarities with the mutational spectrum of G[8,5-Me]T in human embryonic kidney cells.

scholarly journals Mechanisms by which herpes simplex virus DNA polymerase limits translesion synthesis through abasic sites

DNA Repair ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 95-107 ◽  
Author(s):  
Yali Zhu ◽  
Liping Song ◽  
Jason Stroud ◽  
Deborah S. Parris
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Translesion Synthesis ◽  
Abasic Sites ◽  
Simplex Virus

Synthesis of N2-Deoxyguanosine Modified DNAs and the Studies on Their Translesion Synthesis by the E. coli DNA Polymerase IV

2019 ◽  
Vol 84 (4) ◽  
pp. 1734-1747 ◽  
Author(s):  
Pratibha P. Ghodke ◽  
Praneeth Bommisetti ◽  
Deepak T. Nair ◽  
P. I. Pradeepkumar
Keyword(s):  
Dna Polymerase ◽  
Translesion Synthesis ◽  
E Coli ◽  
Dna Polymerase Iv

DNA polymerase κ: Friend or foe?

2020 ◽  
Vol 13 (629) ◽  
pp. eabb2934
Author(s):  
Joann B. Sweasy
Keyword(s):  
Dna Polymerase ◽  
Melanoma Cells ◽  
Adaptation To Stress ◽  
Cellular Adaptation ◽  
Targeted Inhibitors ◽  
Translesion Dna

In this issue of Science Signaling, Temprine et al. report that up-regulation of the translesion DNA polymerase Polκ mediates resistance to BRAF pathway–targeted inhibitors and starvation in melanoma cells. These results exemplify the role that Polκ plays in cellular adaptation to stress.

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