scholarly journals Paper mulberry fruit juice: a novel biomass resource for bioethanol production

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Pleasure Chisom Ajayo ◽  
Mei Huang ◽  
Li Zhao ◽  
Dong Tian ◽  
Qin Jiang ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Fruit Juice ◽  
Ethanol Concentration ◽  
Positive Impact ◽  
Fuel Ethanol ◽  
Fractional Factorial ◽  
Process Conditions ◽  
Mulberry Fruit ◽  
Biomass Resource ◽  
Yeast Concentration

AbstractBy way of broadening the use of diverse sustainable bioethanol feedstocks, the potentials of Paper mulberry fruit juice (PMFJ), as a non-food, sugar-based substrate, were evaluated for fuel ethanol production. The suitability of PMFJ was proven, as maximum ethanol concentration (56.4 g/L) and yield (0.39 g/g) were achieved within half a day of the start of fermentation, corresponding to very high ethanol productivity of 4.7 g/L/hr. The established potentials were further optimally maximized through the response surface methodology (RSM). At the optimal temperature of 30 °C, yeast concentration of 0.55 g/L, and pH of 5, ethanol concentration, productivity, and yield obtained were 73.69 g/L, 4.61 g/L/hr, and 0.48 g/g, respectively. Under these ideal conditions, diverse metal salts were afterward screened for their effects on PMFJ fermentation. Based on a two-level fractional factorial design, nutrient addition had no positive impact on ethanol production. Thus, under the optimal process conditions, and without any external nutrient supplementation, bioethanol from PMFJ compared favorably with typical sugar-based energy crops, highlighting its resourcefulness as a high-value biomass resource for fuel ethanol production. Graphical Abstract

Paper Mulberry Fruit Juice: a Novel Biomass Resource For Bioethanol Production

2021 ◽  
Author(s):  
Pleasure Chisom Ajayo ◽  
Mei Huang ◽  
Li Zhao ◽  
Dong Tian ◽  
Qin Jiang ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Fruit Juice ◽  
Ethanol Concentration ◽  
Fuel Ethanol ◽  
Inoculum Size ◽  
Process Conditions ◽  
Mulberry Fruit ◽  
Biomass Resource ◽  
Yeast Concentration ◽  
First Time

Abstract By way of broadening the use of diverse sustainable bioethanol feedstocks, the potentials of Paper mulberry fruit juice (PMFJ), as a non-food, sugar-based substrate, was for the first time evaluated for fuel ethanol production. Without any external nutrient supplementation, the suitability of PMFJ was proven, as maximum ethanol concentration (56.4 g/L), and yield (0.39 g/g), were achieved within half a day of the start of fermentation, corresponding to a very high ethanol productivity of 4.7 g/L/hr. Using Response Surface Methodology, established potentials were further maximized through statistical optimization of process conditions of temperature (20 – 40 ⁰C), yeast concentration (0.5 – 2 g/L), and pH (4 – 6). At the optimal temperature of 30 ⁰C, inoculum size of 0.55 g/L, and pH of 5, ethanol concentration, productivity, and yield obtained were 73.69 g/L, 4.61 g/L/hr, and 0.48 g/g, respectively. Under this ideal process conditions, bioethanol from PMFJ compares favorably with typical sugar-based energy crops, highlighting its resourcefulness as a high value biomass resource for fuel ethanol production.

scholarly journals Microwave-assisted hydrotropic pretreatment as a new and highly efficient way to cellulosic ethanol production from maize distillery stillage

2021 ◽  
Author(s):  
Mikulski Dawid ◽  
Kłosowski Grzegorz
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Enzymatic Degradation ◽  
Ethanol Concentration ◽  
Process Conditions ◽  
Fermentation Inhibitors ◽  
Microwave Assisted ◽  
Theoretical Yield ◽  
High Level ◽  
Very High

Abstract Aim of the study was to assess the suitability of the combined use of microwave radiation and sodium cumene sulfonate under optimized process conditions for the preparation of maize stillage biomass as a raw material for the production of cellulosic ethanol. The key parameter guaranteeing a high level of lignin removal from biomass (ca. 44%) was concentration of hydrotrope. Even at high biomass concentration (16% w/v) and a cellulase enzyme dose of about 4 filter-paper units/g, maize stillage biomass subjected to microwave-assisted hydrotropic pretreatment was highly susceptible to enzymatic degradation, which resulted in 80% hydrolysis yield. It is possible to obtain a fermentation medium with a very high glucose concentration (up to 80 g/L), without fermentation inhibitors and, as a consequence, to reach a very high level of sugar conversion to ethanol (concentration above 40 g/L), even as much as 95% of theoretical yield. Microwave hydrotropic treatment with sodium cumene sulfonate is a very effective way to prepare waste maize stillage biomass for the production of cellulosic ethanol. The degradation of the lignocellulose structure by the simultaneous use of microwaves and hydrotropes ensured a high degree of conversion of structural polysaccharides to bioethanol. The method provides a high level of enzymatic degradation of cellulose, leading to a medium with high content of released sugars suitable for bioconversion, which is in line with assumptions of the second-generation ethanol production technology. Key points • Microwave-assisted hydrotropic pretreatment is a new way to cellulosic ethanol production. • Microwave-assisted hydrotropic delignification removes 44% of lignin from biomass. • No fermentation inhibitors are obtained after microwave-assisted hydrotropic pretreatment. • High ethanol concentration (above 40 g/L) and fermentation yield (95% of theoretical yield) from biomass after microwave-assisted hydrotropic pretreatment.

scholarly journals Hemicellulosic Bioethanol Production from Fast-Growing Paulownia Biomass

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 173
Author(s):  
Elena Domínguez ◽  
Pablo G. del Río ◽  
Aloia Romaní ◽  
Gil Garrote ◽  
Lucília Domingues
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Xylose Reductase ◽  
Energy Crop ◽  
Scheffersomyces Stipitis ◽  
Fractionation Process ◽  
Renewable Source ◽  
Fast Growing ◽  
Engineered Strain

In order to exploit a fast-growing Paulownia hardwood as an energy crop, a xylose-enriched hydrolysate was obtained in this work to increase the ethanol concentration using the hemicellulosic fraction, besides the already widely studied cellulosic fraction. For that, Paulownia elongata x fortunei was submitted to autohydrolysis treatment (210 °C or S0 of 4.08) for the xylan solubilization, mainly as xylooligosaccharides. Afterwards, sequential stages of acid hydrolysis, concentration, and detoxification were evaluated to obtain fermentable sugars. Thus, detoxified and non-detoxified hydrolysates (diluted or not) were fermented for ethanol production using a natural xylose-consuming yeast, Scheffersomyces stipitis CECT 1922, and an industrial Saccharomyces cerevisiae MEC1133 strain, metabolic engineered strain with the xylose reductase/xylitol dehydrogenase pathway. Results from fermentation assays showed that the engineered S. cerevisiae strain produced up to 14.2 g/L of ethanol (corresponding to 0.33 g/g of ethanol yield) using the non-detoxified hydrolysate. Nevertheless, the yeast S. stipitis reached similar values of ethanol, but only in the detoxified hydrolysate. Hence, the fermentation data prove the suitability and robustness of the engineered strain to ferment non-detoxified liquor, and the appropriateness of detoxification of liquor for the use of less robust yeast. In addition, the success of hemicellulose-to-ethanol production obtained in this work shows the Paulownia biomass as a suitable renewable source for ethanol production following a suitable fractionation process within a biorefinery approach.

scholarly journals Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Morakot Krajang ◽  
Kwanruthai Malairuang ◽  
Jatuporn Sukna ◽  
Krongchan Rattanapradit ◽  
Saethawat Chamsart
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Consolidated Bioprocessing ◽  
Scale Up ◽  
Cassava Starch ◽  
Single Step ◽  
Starch Hydrolysis ◽  
Yield Coefficient ◽  
Raw Starch ◽  
Raw Starch Hydrolysis

Abstract Background A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigate the optimum conditions and technical procedures for the production of ethanol from raw cassava starch in a single step. It successfully resulted in high yields and productivities of all the experiments from the laboratory, the pilot, through the industrial scales. Yields of ethanol concentration are comparable with those in the commercial industries that use molasses and hydrolyzed starch as the raw materials. Results Before single-step ethanol production, studies of raw cassava starch hydrolysis by a granular starch hydrolyzing enzyme, StargenTM002, were carefully conducted. It successfully converted 80.19% (w/v) of raw cassava starch to glucose at a concentration of 176.41 g/L with a productivity at 2.45 g/L/h when it was pretreated at 60 °C for 1 h with 0.10% (v/w dry starch basis) of Distillase ASP before hydrolysis. The single-step ethanol production at 34 °C in a 5-L fermenter showed that Saccharomyces cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, pmax at 81.86 g/L (10.37% v/v) with a yield coefficient, Yp/s of 0.43 g/g, a productivity or production rate, rp at 1.14 g/L/h and an efficiency, Ef of 75.29%. Scale-up experiments of the single-step ethanol production using this method, from the 5-L fermenter to the 200-L fermenter and further to the 3000-L industrial fermenter were successfully achieved with essentially good results. The values of pmax,Yp/s, rp, and Ef of the 200-L scale were at 80.85 g/L (10.25% v/v), 0.42 g/g, 1.12 g/L/h and 74.40%, respectively, and those of the 3000-L scale were at 70.74 g/L (8.97% v/v), 0.38 g/g, 0.98 g/L/h and 67.56%, respectively. Because of using raw starch, major by-products, i.e., glycerol, lactic acid, and acetic acid of all three scales were very low, in ranges of 0.940–1.140, 0.046–0.052, 0.000–0.059 (% w/v), respectively, where are less than those values in the industries. Conclusion The single-step ethanol production using the combination of raw cassava starch hydrolysis and fermentation of three fermentation scales in this study is practicable and feasible for the scale-up of industrial production of ethanol from raw starch.

Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium

2012 ◽  
Vol 160 (3-4) ◽  
pp. 229-235 ◽  
Author(s):  
Yu Shen ◽  
Jin-Song Guo ◽  
You-Peng Chen ◽  
Hai-Dong Zhang ◽  
Xu-Xu Zheng ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Nitrogen Source ◽  
Sweet Potato ◽  
Low Cost ◽  
Fuel Ethanol ◽  
High Gravity

Optimizing Liquid Hot Water pretreatment conditions to enhance sugar recovery from wheat straw for fuel-ethanol production

Fuel ◽  
2008 ◽  
Vol 87 (17-18) ◽  
pp. 3640-3647 ◽  
Author(s):  
J.A. Pérez ◽  
I. Ballesteros ◽  
M. Ballesteros ◽  
F. Sáez ◽  
M.J. Negro ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Fuel Ethanol ◽  
Hot Water ◽  
Sugar Recovery ◽  
Liquid Hot Water Pretreatment ◽  
Liquid Hot Water ◽  
Water Pretreatment ◽  
Pretreatment Conditions ◽  
Hot Water Pretreatment

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Full-scale On-farm Pretreatment of Perennial Grasses with Dilute Acid for Fuel Ethanol Production

2010 ◽  
Vol 3 (4) ◽  
pp. 335-341 ◽  
Author(s):  
Matthew Francis Digman ◽  
Kevin J. Shinners ◽  
Richard E. Muck ◽  
Bruce S. Dien
Keyword(s):  
Ethanol Production ◽  
Fuel Ethanol ◽  
Full Scale ◽  
Perennial Grasses ◽  
Dilute Acid ◽  
On Farm
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