scholarly journals Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Balazs Feher ◽  
Karol Ali Apaza Alccayhuaman ◽  
Franz Josef Strauss ◽  
Jung-Seok Lee ◽  
Stefan Tangl ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Collagen Membrane ◽  
Bony Defect ◽  
Dense Layer ◽  
Type I ◽  
Trabecular Thickness ◽  
Defect Area ◽  
Collagen Membranes

Abstract Background Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes with their dense layer towards the soft tissue and their porous layer towards the bony defect area. However, evidence supporting this recommendation is lacking. This study aimed to determine whether the alignment of bilayer collagen membranes has an effect on bone regeneration. Methods In two groups of ten male Sprague-Dawley rats each, a 5-mm calvarial defect was created. Thereafter, the defect was randomly covered with a bilayer, resorbable, pure type I and III collagen membrane placed either regularly or upside-down (i.e., dense layer towards bone defect). After 4 weeks of healing, micro-computed tomography (μCT), histology, and histomorphometry of the inner cylindrical region of interest (4.5 mm in diameter) were performed to assess new bone formation and the consolidation of the collagen membrane in the defect area. Results Quantitative μCT showed similar bone volume (median 8.0 mm3, interquartile range 7.0–10.0 vs. 6.2 mm3, 4.3–9.4, p = 0.06) and trabecular thickness (0.21 mm, 0.19–0.23 vs. 0.18 mm, 0.17–0.20, p = 0.03) between upside-down and regular placement, both leading to an almost complete bony coverage. Histomorphometry showed comparable new bone areas between the upside-down and regularly placed membranes, 3.9 mm2 (2.7–5.4) vs. 3.8 mm2 (2.2–4.0, p = 0.31), respectively. Both treatment groups revealed the same regeneration patterns and spatial distribution of bone with and without collagen fibers, as well as residual collagen fibers. Conclusions Our data support the osteoconductive properties of collagen membranes and suggest that bone regeneration is facilitated regardless of membrane layer alignment.

scholarly journals Dentin-Derived-Barrier Membrane in Guided Bone Regeneration: A Case Report

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2166
Author(s):  
Jeong-Kui Ku ◽  
In-Woong Um ◽  
Mi-Kyoung Jun ◽  
Il-hyung Kim
Keyword(s):  
Case Report ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Successful Outcome ◽  
Collagen Membrane ◽  
Type I ◽  
Barrier Membrane ◽  
Dentin Matrix ◽  
Demineralized Dentin Matrix ◽  
Demineralized Dentin

An autogenous, demineralized, dentin matrix is a well-known osteo-inductive bone substitute that is mostly composed of type I collagen and is widely used in implant dentistry. This single case report describes a successful outcome in guided bone regeneration and dental implantation with a novel human-derived collagen membrane. The authors fabricated a dentin-derived-barrier membrane from a block-type autogenous demineralized dentin matrix to overcome the mechanical instability of the collagen membrane. The dentin-derived-barrier acted as an osteo-inductive collagen membrane with mechanical and clot stabilities, and it replaced the osteo-genetic function of the periosteum. Further research involving large numbers of patients should be conducted to evaluate bone forming capacity in comparison with other collagen membranes.

The Effects of Collagen Membrane Coated with PLGA on Bone Regeneration in Rat Calvarial Defects

2007 ◽  
Vol 342-343 ◽  
pp. 357-360
Author(s):  
Kun Young Song ◽  
Yoo Jung Um ◽  
Ui Won Jung ◽  
Yong Keun Lee ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Collagen Membrane ◽  
Histomorphometric Analysis ◽  
Local Bone ◽  
Calvarial Defects ◽  
Formation Behavior ◽  
Surgical Implantation ◽  
Collagen Membranes ◽  
Rat Calvarial Defect

The purpose of this study was to evaluate the effects of collagen membrane coated with PLGA on bone regeneration in rat calvarial defect. Five groups of 10 animals each received either collagen membrane coated with 0.5%, 1%, 3% concentration of PLGA, collagen membrane only or surgical control. Each group of animals was healed into 2 healing periods of 2(5 animals) and 8(5 animals)weeks and histologic and histomorphometric analysis were done. The results of the following study revealed that surgical implantation of collagen membranes coated with PLGA enhanced local bone formation at both 2 and 8 weeks independent of different PLGA concentrations. In conclusion, collagen membrane coated with PLGA shows a significant bone formation behavior irrespective of their concentration.

scholarly journals 4138 Development of an Antibiofilm Resorbable Membrane for Treating Peri-implantitis

2020 ◽  
Vol 4 (s1) ◽  
pp. 121-121
Author(s):  
Zhou Ye ◽  
Joseph R. Rahimi ◽  
Nicholas G. Fischer ◽  
Hooi Pin Chew ◽  
Conrado Aparicio
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Statistical Significance ◽  
Oral Bacteria ◽  
Collagen Fibers ◽  
Collagen Membrane ◽  
Type I ◽  
Success Rates ◽  
Hydrophobic Membrane ◽  
Water Contact

OBJECTIVES/GOALS: Peri-implantitis is the inflammation of peri-implant mucosa and subsequent loss of supporting bone. Its treatment is only <40% successful mainly due to persistent bacterial infection. The goal of this project is to increase success rates by developing a robust antibiofilm multi-biomolecular membrane that can be placed around implant surfaces. METHODS/STUDY POPULATION: A collagen membrane was soaked in the antimicrobial peptide GL13K solution overnight to form an interpenetrating fibrillary network. The nanostructure of the membrane was imaged with scanning electron microscope (SEM). The hydrophobicity of the membrane was analyzed by water contact angle (WCA) measurements. The biodegradability was tested in a 0.01 mg/mL Type I collagenase solution for up to 5 weeks. The antimicrobial activity of the membrane was assessed with Gram-positive oral bacteria Streptococcus gordonii. The cytotoxicity was evaluated by culturing human gingival fibroblasts (HGF), and the osteogenesis was assessed using preosteoblasts MC3T3. Pure collagen membrane was used as the control. Statistical significance (p<0.05) was determined by one-way ANOVA with Tukey’s HSD test. RESULTS/ANTICIPATED RESULTS: The antimicrobial peptide GL13K self-assembled to short fibrils (< 1 µm long), which entangled with the larger collagen fibers (around 200 nm in diameter). The collagen fibers presented characteristic periodic banding structures, which provided biomimetic cues for cell behavior as extracellular matrix. The interpenetrated GL13K fibrils turned the highly hydrophilic collagen membrane to a hydrophobic membrane (WCA = 135 °) and significantly reduced the rate of degradation by collagenases. The developed membrane was efficient in preventing the attachment of S. gordonii. A large portion of the attached bacteria was killed on the surface of the membrane. The incorporation of GL13K did not affect the cytocompatibility of the membrane for HGF. DISCUSSION/SIGNIFICANCE OF IMPACT: We developed an antibiofilm membrane with interpenetrating collagen and antimicrobial peptide fibrils. The strong antimicrobial activity and low cytotoxicity support its further translational evaluation as scaffolds for increasing success rate in treating peri-implantitis.

scholarly journals Guided Bone Regeneration- A Comprehensive Review

2021 ◽  
Author(s):  
Vineetha Venugopalan ◽  
Anegundi Raghavendra Vamsi ◽  
Santhosh Shenoy ◽  
Karishma Ashok ◽  
Biju Thomas
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Primary Stability ◽  
Guided Bone Regeneration ◽  
Collagen Membrane ◽  
Bony Defect ◽  
Graft Material ◽  
Bone Augmentation ◽  
Wide Range ◽  
Space Maintenance

Successful implant treatment requires prosthetically driven placement of an implant, primary stability at placement, and careful living bone management. The resorptive changes of alveolar bone are an inevitable process following tooth loss, periodontal disease or trauma which causes bone defects. This results in various aesthetic and functional complications such as soft tissue recession, infection and inflammation. Various methods have been tried and advocated for augmenting these bone deficiencies. Guided Bone Regeneration (GBR) is a successful modality for bone augmentation with a wide range of indications and helps restore the alveolar ridge dimensions. It utilises the principle of Guided Tissue Regeneration (GTR) for space maintenance within a bony defect. Different types of barrier membranes are being utilised along with various bone grafts in GBR. Thorough knowledge regarding the biology of bone is required before the initiation of any bone augmentation procedure. A combination of Collagen Membrane (CM) and graft material was found successful for GBR. Hence, this review focuses on presentation of best available evidence for various aspects of GBR.

scholarly journals Evaluation of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Cross-Linked Collagen Membranes for Guided Bone Regeneration in Beagle Dogs

Materials ◽  
2020 ◽  
Vol 13 (20) ◽  
pp. 4599
Author(s):  
Jong-Ju Ahn ◽  
Hyung-Joon Kim ◽  
Eun-Bin Bae ◽  
Won-Tak Cho ◽  
YunJeong Choi ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Physical Properties ◽  
Bone Regeneration ◽  
Enzymatic Degradation ◽  
Test Group ◽  
Guided Bone Regeneration ◽  
Control Group ◽  
Collagen Membrane ◽  
Micro Computed Tomography ◽  
Collagen Membranes

The purpose of this study was to evaluate the bone regeneration efficacy of an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked collagen membrane for guided bone regeneration (GBR). A non-cross-linked collagen membrane (Control group), and an EDC-cross-linked collagen membrane (Test group) were used in this study. In vitro, mechanical, and degradation testing and cell studies were performed. In the animal study, 36 artificial bone defects were formed in the mandibles of six beagles. Implants were inserted at the time of bone grafting, and membranes were assigned randomly. Eight weeks later, animals were sacrificed, micro-computed tomography was performed, and hematoxylin-eosin stained specimens were prepared. Physical properties (tensile strength and enzymatic degradation rate) were better in the Test group than in the Control group. No inflammation or membrane collapse was observed in either group, and bone volumes (%) in defects around implants were similar in the two groups (p > 0.05). The results of new bone areas (%) analysis also showed similar values in the two groups (p > 0.05). Therefore, it can be concluded that cross-linking the collagen membranes with EDC is the method of enhancing the physical properties (tensile strength and enzymatic degradation) of the collagen membranes without risk of toxicity.

scholarly journals In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes

Membranes ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 423
Author(s):  
Abdu Mansur Dacache Neto ◽  
Suelen Cristina Sartoretto ◽  
Isabelle Martins Duarte ◽  
Rodrigo Figueiredo de Brito Resende ◽  
Adriana Terezinha Neves Novellino Alves ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Guided Bone Regeneration ◽  
Tissue Reaction ◽  
Clinical Indication ◽  
Collagen Membrane ◽  
Tissue Reactions ◽  
Porcine Collagen

Mechanical barriers prevent the invasion of the surrounding soft tissues within the bone defects. This concept is known as Guided Bone Regeneration (GBR). The knowledge about the local tissue reaction and the time of degradation of absorbable membranes favors the correct clinical indication. This study aimed to evaluate the biocompatibility and biodegradation of a bovine collagen membrane (Lyostypt®, São Gonçalo, Brazil) and compare it to a porcine collagen membrane (Bio-Gide®) implanted in the subcutaneous tissue of mice, following ISO 10993-6:2016. Thirty Balb-C mice were randomly divided into three experimental groups, LT (Lyostypt®), BG (Bio-Gide®), and Sham (without implantation), and subdivided according to the experimental periods (7, 21, and 63 days). The BG was considered non-irritant at seven days and slight and moderate irritant at 21 and 63 days, respectively. The LT presented a small irritant reaction at seven days, a mild reaction after 21, and a reduction in the inflammatory response at 63 days. The biodegradation of the LT occurred more rapidly compared to the BG after 63 days. This study concluded that both membranes were considered biocompatible since their tissue reactions were compatible with the physiological inflammatory process; however, the Bio-Gide® was less degraded during the experimental periods, favoring the guided bone regeneration process.

scholarly journals Implant Site Guided Bone Regeneration and Pontic Site Ridge Preservation: A Case Report

PRILOZI ◽  
2021 ◽  
Vol 42 (2) ◽  
pp. 103-108
Author(s):  
Darko Veljanovski ◽  
Denis Baftijari ◽  
Zoran Susak ◽  
Aneta Atanasovska Stojanovska
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Soft Tissues ◽  
Guided Bone Regeneration ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Ridge Preservation ◽  
Bovine Bone ◽  
Implant Placement ◽  
Barrier Membrane

Abstract Guided bone regeneration (GBR) is a therapeutic modality to achieve bone regeneration with the use of barrier membranes. The use of deproteinized bovine bone material (DBBM) for ridge preservation allows the preservation of the edentulous ridge dimensions. Here, we present a case of horizontal GBR using DBBM and a resorbable membrane, with simultaneous implant placement. Simultaneously, ridge preservation of the pontic area, using DBBM within a “socket seal” procedure was performed. Two implants were places at sites 23 and 26 to support a fixed partial denture (FPD). The mesial implant showed exposed buccal threads, which were then covered with autogenous bone particles and small size granules of DBBM. The collagen membrane was stabilized with periosteal mattress suture. Six months postoperatively, CBCT images revealed a stable buccal bone layer at the implant site, indicating a successful GBR procedure. At this point in time, tooth 24 was atraumatically extracted. A ridge preservation was done utilizing DBBM, and a soft tissue graft form the tuber. A ceramic-metal FPD with excellent “white aesthetics” and a harmonic transition zone to the soft tissue was fabricated. At 3 years follow up, the peri-implant bone levels were stable, and the clinical outcomes were excellent. It is concluded that a GBR procedure, utilizing DBBM and a collagen barrier membrane with simultaneous implant placement, as well as ridge preservation using DBBM, are predictable therapeutic methods. However, gentle manipulation of the soft tissues, and wound stability, with tension-free passive closure of the wound margins are prerequisites for a long-term clinical success.

scholarly journals A Rapid and Convenient Approach to Construct Porous Collagen Membranes via Bioskiving and Sonication-Feasible for Mineralization to Induce Bone Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Zhenzhen Wu ◽  
Juan Zhong ◽  
Yingjie Yu ◽  
Mingdeng Rong ◽  
Tao Yang
Keyword(s):  
Bone Regeneration ◽  
Porous Membrane ◽  
Collagen Membrane ◽  
Power Intensity ◽  
Combined Application ◽  
Collagen Denaturation ◽  
Convenient Approach ◽  
Novel Method ◽  
Collagen Membranes ◽  
Mineralized Collagen

Porous mineralized collagen membranes efficiently promote bone regeneration. To generate them, we need to fabricate collagen membranes that are porous. However, the current fabrication method is primarily based on a bottom-up strategy, with certain limitations, such as a long manufacturing process, collagen denaturation, and failure to control fibril orientation. Using a top-down approach, we explore a novel method for constructing porous collagen membranes via the combined application of bioskiving and sonication. Numerous collagen membranes with well-aligned fibril structures were rapidly fabricated by bioskiving and then sonicated at 30, 60, 90, and 120 W for 20 min. This treatment allowed us to study the effect of power intensity on the physicochemical traits of collagen membranes. Subsequently, the prepared collagen membranes were immersed in amorphous calcium phosphate to evaluate the feasibility of mineralization. Additionally, the bioactivities of the membranes were assessed using preosteoblast cells. Tuning the power intensity was shown to modulate fibril orientation, and the porous membrane without denatured collagen could be obtained by a 20-min sonication treatment at 90 W. The prepared collagen membrane could also be further mineralized to enhance osteogenesis. Overall, this study offers a rapid and convenient approach for fabricating porous collagen membranes via bioskiving and sonication.

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