scholarly journals Recent progress and perspectives on the mechanisms underlying Asbestos toxicity

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Akio Kuroda
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Inflammatory Responses ◽  
Imaging Techniques ◽  
Ambient Air ◽  
Mesothelial Cells ◽  
Intracellular Uptake ◽  
Genetic Studies ◽  
Asbestos Fibers ◽  
Frustrated Phagocytosis

AbstractMost cases of mesothelioma are known to result from exposure to asbestos fibers in the environment or occupational ambient air. The following questions regarding asbestos toxicity remain partially unanswered: (i) why asbestos entering the alveoli during respiration exerts toxicity in the pleura; and (ii) how asbestos causes mesothelioma, even though human mesothelial cells are easily killed upon exposure to asbestos. As for the latter question, it is now thought that the frustrated phagocytosis of asbestos fibers by macrophages prolongs inflammatory responses and gives rise to a “mutagenic microenvironment” around mesothelial cells, resulting in their malignant transformation. Based on epidemiological and genetic studies, a carcinogenic model has been proposed in which BRCA1-associated protein 1 mutations are able to suppress cell death in mesothelial cells and increase genomic instability in the mutagenic microenvironment. This leads to additional mutations, such as CDKN2A [p16], NF2, TP53, LATS2, and SETD2, which are associated with mesothelioma carcinogenesis. Regarding the former question, the receptors involved in the intracellular uptake of asbestos and the mechanism of transfer of inhaled asbestos from the alveoli to the pleura are yet to be elucidated. Further studies using live-cell imaging techniques will be critical to fully understanding the mechanisms underlying asbestos toxicity.

scholarly journals Holotomography: refractive index as an intrinsic imaging contrast for 3-D label-free live cell imaging

2017 ◽  
Author(s):  
Doyeon Kim ◽  
SangYun Lee ◽  
Moosung Lee ◽  
JunTaek Oh ◽  
Su-A Yang ◽  
...  
Keyword(s):  
Refractive Index ◽  
Cell Biology ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Imaging Techniques ◽  
Live Cell ◽  
Label Free ◽  
Essential Information ◽  
Research Fields ◽  
Intrinsic Imaging

AbstractLive cell imaging provides essential information in the investigation of cell biology and related pathophysiology. Refractive index (RI) can serve as intrinsic optical imaging contrast for 3-D label-free and quantitative live cell imaging, and provide invaluable information to understand various dynamics of cells and tissues for the study of numerous fields. Recently significant advances have been made in imaging methods and analysis approaches utilizing RI, which are now being transferred to biological and medical research fields, providing novel approaches to investigate the pathophysiology of cells. To provide insight how RI can be used as an imaging contrast for imaging of biological specimens, here we provide the basic principle of RI-based imaging techniques and summarize recent progress on applications, ranging from microbiology, hematology, infectious diseases, hematology, and histopathology.

Cucurbit[7]uril-conjugated dyes as live cell imaging probes: investigation on their cellular uptake and excretion pathways

2019 ◽  
Vol 17 (25) ◽  
pp. 6215-6220 ◽  
Author(s):  
Meng Li ◽  
Ara Lee ◽  
Sungwan Kim ◽  
Annadka Shrinidhi ◽  
Kyeng Min Park ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Intracellular Uptake ◽  
Imaging Probes

The potential of cucurbit[7]uril derivatives as live cell imaging probes is demonstrated by investigating the intracellular uptake and excretion of dye-conjugated cucurbit[7]uril derivatives.

Expansion Microscopy: Scalable and Convenient Super-Resolution Microscopy

2019 ◽  
Vol 35 (1) ◽  
pp. 683-701 ◽  
Author(s):  
Paul W. Tillberg ◽  
Fei Chen
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Resolving Power ◽  
Relative Ease ◽  
Nanoscale Structures ◽  
Physical Form ◽  
Tissue Specimens ◽  
Super Resolution Microscopy

Expansion microscopy (ExM) is a physical form of magnification that increases the effective resolving power of any microscope. Here, we describe the fundamental principles of ExM, as well as how recently developed ExM variants build upon and apply those principles. We examine applications of ExM in cell and developmental biology for the study of nanoscale structures as well as ExM's potential for scalable mapping of nanoscale structures across large sample volumes. Finally, we explore how the unique anchoring and hydrogel embedding properties enable postexpansion molecular interrogation in a purified chemical environment. ExM promises to play an important role complementary to emerging live-cell imaging techniques, because of its relative ease of adoption and modification and its compatibility with tissue specimens up to at least 200 μm thick.

scholarly journals Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell

2012 ◽  
Vol 197 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Wei Li ◽  
Wei Zou ◽  
Yihong Yang ◽  
Yongping Chai ◽  
Baohui Chen ◽  
...  
Keyword(s):  
Autoimmune Disease ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Apoptotic Cell ◽  
Imaging Techniques ◽  
Time Lapse ◽  
Apoptotic Cell Clearance ◽  
Cell Clearance ◽  
Fundamental Process ◽  
Cell Corpse

Apoptotic cell degradation is a fundamental process for organism development, and impaired clearance causes inflammatory or autoimmune disease. Although autophagy genes were reported to be essential for exposing the engulfment signal on apoptotic cells, their roles in phagocytes for apoptotic cell removal are not well understood. In this paper, we develop live-cell imaging techniques to study apoptotic cell clearance in the Caenorhabditis elegans Q neuroblast lineage. We show that the autophagy proteins LGG-1/LC3, ATG-18, and EPG-5 were sequentially recruited to internalized apoptotic Q cells in the phagocyte. In atg-18 or epg-5 mutants, apoptotic Q cells were internalized but not properly degraded; this phenotype was fully rescued by the expression of autophagy genes in the phagocyte. Time-lapse analysis of autophagy mutants revealed that recruitment of the small guanosine triphosphatases RAB-5 and RAB-7 to the phagosome and the formation of phagolysosome were all significantly delayed. Thus, autophagy genes act within the phagocyte to promote apoptotic cell degradation.

Mitochondria- and nucleolus-targeted copper(i) complexes with pyrazole-linked triphenylphosphine moieties for live cell imaging

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Mayank Mayank ◽  
Richa Rani ◽  
Ashutosh Singh ◽  
Neha Garg ◽  
Navneet Kaur ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Imaging Techniques ◽  
Live Cell ◽  
Single Experiment ◽  
Highly Efficient

We developed C1 and C2 metallo-complexes as highly efficient cell imaging tools. These complexes enabled us to visualize mitochondria and nucleolus in a single experiment via live cell imaging techniques.

Live Cell-Imaging Techniques for Analyses of Microtubules in Dictyostelium

2010 ◽  
pp. 341-357 ◽  
Author(s):  
Matthias Samereier ◽  
Irene Meyer ◽  
Michael P. Koonce ◽  
Ralph Gräf
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Imaging Techniques ◽  
Live Cell

Long-term live-cell imaging techniques for visualizing pavement cell morphogenesis

2020 ◽  
pp. 365-380
Author(s):  
Kumar Seerangan ◽  
Ruben van Spoordonk ◽  
Arun Sampathkumar ◽  
Ryan Christopher Eng
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Imaging Techniques ◽  
Live Cell ◽  
Cell Morphogenesis ◽  
Pavement Cell

scholarly journals Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Takenori Ishida ◽  
Nobutoshi Fujihara ◽  
Tomoki Nishimura ◽  
Hisakage Funabashi ◽  
Ryuichi Hirota ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Asbestos Fibers ◽  
Macrophage Phagocytosis
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