Nephrotoxicity and its prevention by catechin in ferric nitrilotriacetate promoted oxidative stress in rats

2004 ◽  
Vol 23 (3) ◽  
pp. 137-143 ◽  
Author(s):  
Kanwaljit Chopra ◽  
Devinder Singh ◽  
Vikas Chander
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Renal Dysfunction ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Morphological Alterations ◽  
Group Iii ◽  
Ferric Nitrilotriacetate ◽  
Group I ◽  
Rats And Mice

Intraperitoneal injection of ferric nitrilotriacetate (Fe-NTA) to rats and mice results in iron-induced free radical injury and cancer in kidneys. This study was designed to investigate the effect of catechin, a bioflavonoid with antioxidant potential, on Fe-NTA-induced nephrotoxicity in rats. Four groups were employed in the present study. Group I served as control group, Group II animals received Fe-NTA (8 mg iron/kg body weight i.p.), Group III animals were given 40 mg/kg catechin p.o. twice a day for 4 days and on the 5th day Fe-NTA was challenged, and Group IV animals received catechin alone for 4 days. Renal function was assessed by measuring plasma creatinine and blood urea nitrogen. The oxidative stress was measured by renal malondialdehyde levels, reduced glutathione levels and by enzymatic activity of catalase, glutathione reductase and superoxide dismutase. One hour after a single intraperitoneal (i.p.) injection of Fe-NTA (8 mg iron/kg), a marked deterioration of renal architecture, renal function and severe oxidative stress was observed. Pretreatment of animals with catechin markedly attenuated renal dysfunction, reduced elevated thiobarbituric acid reacting substances (TBARS), restored the depleted renal antioxidant enzymes and normalized the renal morphological alterations. These results clearly demonstrate the role of oxidative stress and its relation to renal dysfunction, and suggest a protective effect of catechin on Fe-NTA-induced nephrotoxicity in rats.

scholarly journals Thymoquinone Lowers Blood Glucose and Reduces Oxidative Stress in a Rat Model of Diabetes

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2348
Author(s):  
Mohamed Faisal Lutfi ◽  
Abdel-Moneim Hafez Abdel-Moneim ◽  
Ashwag Saleh Alsharidah ◽  
Mugahid A. Mobark ◽  
Ahmed A. H. Abdellatif ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Glycaemic Control ◽  
Histopathological Examination ◽  
Glycated Haemoglobin ◽  
Diabetic Rats ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Lipid Peroxidase

The aim of the present study was to assess the short-term effects of Thymoquinone (TQ) on oxidative stress, glycaemic control, and renal functions in diabetic rats. DM was induced in groups II and III with a single dose of streptozotocin (STZ), while group I received no medication (control). The rats in groups I and II were then given distilled water, while the rats in group III were given TQ at a dose of 50 mg/kg body weight/day for 4 weeks. Lipid peroxidase, nitric oxide (NO), total antioxidant capacity (TAC), glycated haemoglobin (HbA1c), lipid profiles, and renal function were assessed. Moreover, the renal tissues were used for histopathological examination. STZ increased the levels of HbA1c, lipid peroxidase, NO, and creatinine in STZ-induced diabetic rats in comparison to control rats. TAC was lower in STZ-induced diabetic rats than in the control group. Furthermore, rats treated with TQ exhibited significantly lower levels of HbA1c, lipid peroxidase, and NO than did untreated diabetic rats. TAC was higher in diabetic rats treated with TQ than in untreated diabetic rats. The histopathological results showed that treatment with TQ greatly attenuated the effect of STZ-induced diabetic nephropathy. TQ effectively adjusts glycaemic control and reduces oxidative stress in STZ-induced diabetic rats without significant damaging effects on the renal function.

scholarly journals Determination of thiol/disulphide homeostasis as a new indicator of oxidative stress in dairy cows with subclinical endometritis

2021 ◽  
Vol 91 (2) ◽  
pp. 137-148
Author(s):  
Birten Emre ◽  
◽  
Ömer Korkmaz ◽  
Ismail Koyuncu ◽  
Selim Çomaklı ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Immunocytochemical Staining ◽  
Control Group ◽  
Roc Curve Analysis ◽  
Group Iii ◽  
Total Thiol ◽  
Group I ◽  
Subclinical Endometritis

The objective of this study was to determine thiol/disulphide homeostasis (TDH) in infertile cows with subclinical endometritis (SCE). Endometrial cytological samples were collected using a cytobrush to diagnose SCE in 36 infertile cows. According to the results of the cytology examination, those with acute endometritis were classified as Group I (n = 20) and those with chronic endometritis were classified as Group II (n = 16). A control group was formed of heifers as Group III (n = 20). Blood samples were taken from each group on the day of diagnosis (day 0) to analyse TDH. In the cytology examination, both the Giemsa method and immunocytochemical staining were applied to determine chronic inflammation and activity status. In 55.55% (20/36) of the infertile cows with cytological endometritis, the inflammation was determined to be active, and in 44.44% (16/36) it had become chronic. The native thiol and total thiol levels were found to be statistically significantly lower in the acute (206.54 ± 8.30 μmol/L; 227.11 ± 9.30 μmol/L) and chronic SCE cases (225.15 ± 11.89 μmol/L; 247.96 ± 10.80 μmol/L) compared to the heathy control group (308.47 ± 13.59 μmol/L; 336.83 ± 15.5 μmol/L respectively) (P<0.001). Disulphide levels, disulphide/total thiol, native thiol/total thiol and disulphide/native thiol ratios were similar in all the groups (P>0.05). The diagnostic accuracy of native thiol, which can be used in the diagnosis of SCE, was 92.8%, that of total thiol was 89.3% and that of disulphide was 64.3% according to the ROC curve analysis. These results demonstrate that TDH is a reliable and sensitive indicator of oxidative stress in cow SCE, and that abnormal TDH might play a role in SCE pathogenic mechanisms. This is the first study to evaluate thiol/disulphide homeostasis in dairy cows with SCE as a new indicator of oxidative stress.

scholarly journals Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Akheruz Zaman Ahmed ◽  
Shakta Mani Satyam ◽  
Prakashchandra Shetty ◽  
Melanie Rose D’Souza
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Methyl Gallate ◽  
Group Iii ◽  
Group I ◽  
Female Wistar Rats ◽  
Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p  < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p  < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

scholarly journals Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

2017 ◽  
Vol 45 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Andrei CONEAC ◽  
Meda Sandra ORASAN ◽  
Daniel Corneliu LEUCUTA ◽  
Nicoleta DECEA ◽  
Miuta FILIP ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Experimental Inflammation ◽  
Group Ii

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

scholarly journals AB0053 THE ROLE OF ANGIOPOIETIN-LIKE PROTEINS TYPES 3 AND 4 IN THE DEVELOPMENT OF RENAL DYSFUNCTION IN PATIENTS WITH RHEUMATOID ARTHRITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1059.1-1059
Author(s):  
V. Aleksandrov ◽  
L. Shilova ◽  
A. Aleksandrov
Keyword(s):  
Rheumatoid Arthritis ◽  
Renal Function ◽  
Glomerular Filtration Rate ◽  
Kidney Disease ◽  
Renal Dysfunction ◽  
Filtration Rate ◽  
Serum Concentrations ◽  
Group Iii ◽  
Group I

Background:Rheumatoid arthritis (RA) often contributes to the development of kidney disease. Angiopoietin-like proteins can be target markers for studying cardiorenal complications of RA [1].Objectives:Assessment of the correlation of serum concentrations of angiopoietin-like proteins types 3 and 4 (ANGPTL 3 and 4) with the progression of renal dysfunction in RA patients.Methods:114 patients with reliable RA (90.4% of women, 9.6% of men) aged 21 to 80 years (mean age 55.4 ± 11.2 years old), disease duration - 11.18 ± 9.03 years, positive for rheumatoid factor (RF-IgM) - 63.2%, positive for anti-citrullinated protein antibody (ACPA) - 59.7%) were examined. The laboratory examination included the determination of serum concentrations of angiopoietin-like protein type 3 (Human Angiopoietin-like Protein 3 ELISA, Bio Vendor, Czech Republic) and type 4 (RayBio Human ANGPTL4 ELISA Kit; RayBiotech, USA). To assess renal function in RA patients we used the calculated glomerular filtration rate (GFR) according to the CKD-EPI formula [2], taking into account the height and weight of a particular patient without indexing by body surface area. GFR values <60 ml / min / 1.73 m2 were regarded as a certain decrease, and GFR values from 60 to 89 ml / min / 1.73 m2 - as a slight decrease in global kidney function.Results:The concentration of ANGPTL3 in the blood serum of RA patients (n = 158) was 641.9 ± 224.5 ng / ml, and that of ANGPTL4 (n = 158) - 3.15 [0.77; 12.1] ng / ml. 74.7% (n = 118) were considered positive for the presence of ANGPTL3; 49.4% (n = 78) of RA patients were recognized as positive for the presence of ANGPTL4. The average glomerular filtration rate in RA patients was 74.0 ± 18.6 ml / min. More than а half of the examined RA patients had GFR ranging from 89 to 60 ml / min / 1.73 m2 (C1 - 21.5%; C2 - 58.9%; C3 - 19.6%). No sharp reduce of renal function (GFR <30 ml / min / 1.73 m2) corresponding to CKD C4-5 stages was recorded. Negative correlations of average strength were found between GFR and the level of ANGPTL 3 (r = -0.32, p <0.001) and ANGPTL 4 (rS = -0.31, p <0.001), as well as with age (rS = -0.28, p <0.001), the duration of RA (rS = -0.22, p = 0.005) and blood pressure increase (rS = -0.25, p = 0.001). On the basis of GFR measurements, patients were divided into three groups: group I - optimal renal function (> 90 ml / min); group II - a slight decrease in renal function (89-60 ml / min); group III - reduced renal function (<59 ml / min).Table 1.Content of ANGPTL 3 and 4 in RA patients with different GFR, ng / mlGroup I (n=34)Group II (n=93)Group III (n=31)ANGPTL 3533,4±161,7 I-III650,0±223,9733,2±244,1ANGPTL 40,77 [0,28;3,6] I-II, I-III3,3 [0,93;12,1]6,48 [1,52;19,3]Note: upper case indicates intergroup differences at p <0.05.There was a significant difference in the content of ANGPTL3 in patients of the first group and the patients of the third group (H-test = 6.55, p = 0.032) and ANGPTL4 in the group of patients with normal renal function (group I) and groups of RA patients with decreased GFR (group I- II: H-test = 10.7, p = 0.001; groups I-III: H-test = 20.1, p <0.001). ANGPTL4 indices also had intergroup differences (groups II-III: H-test = 7.2, p = 0.007) with GFR less than 90 ml / min.Conclusion:Chronic rheumatoid inflammation potentiates the development of renal dysfunction according to our data in 78.5% of patients. It is also accompanied by an increase in the content of ANGPTL types 3 and 4 in the blood of RA patients. A better understanding of the actions and mechanisms of ANGPTL may be of paramount importance for the development of effective ways of treatment for cardiorenal complications in RA.References:[1]Aleksandrov A., Aleksandrov V., Shilova L. Study of the role of angiopoietin-like protein type 4 in metabolic disorders caused by inflammation in rheumatoid arthritis. Ann Rheum Dis. 2020;79(s1):1341. doi: 10.1136/annrheumdis-2020-eular.4558.[2]KDIGO 2012 clinical practice guideline for the evaluation and management of chronic kidney disease. Kidney Int Suppl. 2013;3:1–150.Disclosure of Interests:None declared

scholarly journals Experimental Evaluation of Prophylactic and Therapeutic Antioxidant Potential of Trimetazidine in Carbon Tetrachloride Induced Oxidative Stress in Rats

2021 ◽  
Author(s):  
Vijay Haribhau Mate ◽  
Vijaya Anil Pandit ◽  
Pradnya Hemant Padalkar ◽  
Chetan Shrirang More ◽  
Kapil S Khade
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Antioxidant Potential ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Group Iii ◽  
Group I

Introduction: Exposure to various drugs and chemicals lead to oxidative stress. Carbon Tetrachloride (CCl4) produces rise in oxidative stress leading to hepatic damage. The drug Trimetazidine (TMZ) shows hepatoprotective activity but its mechanism is not known. The present study would help in establishing antioxidant activity of TMZ as probable mechanism. Aim: To evaluate the antioxidant potential of TMZ in CCl4 induced oxidative stress when given prophylactically/therapeutically in rats. Materials and Methods: An experimental animal study was conducted on 80 adult Wistar rats of either sex (weight-150 to 200 gm) from March 2010 to December 2010 in Bharati Vidyapeeth Medical College, Pune, Maharashtra, India. Randomly, all animals were grouped into 10 equal groups. Group i was normal control (received only water). To induce oxidative stress CCl4 (0.5 mL/kg/d i.p.) was given to all the animals of Group ii to Group x for seven days. The TMZ was given in two doses, TMZ1 (5 mg/kg orally for Group iii and vii) and TMZ2 (10 mg/kg orally for Group iv and viii). Positive standard control (Group v and Group ix) received Liv.52 (1 mL/kg orally). Group vi and Group x received combination of TMZ1 (5 mg/kg orally)+Liv.52 (1 mL/kg orally). Drug treatment was given to animals in group iii, iv, v and vi for 1-14 days (preventive group) and in group vii, viii, ix and x from day 8 to day 14 (therapeutic group). On 15th day, rats were sacrificed and dissected for collection of liver. Part of the livers was homogenised to assess oxidative stress marker enzymes Malondialdehyde (MDA), Superoxide Dismutase (SOD) spectrophotometrically. Statistical analysis was done with one- way Analysis of Variance (ANOVA) followed by post-hoc analysis (Dunnett’s test) using GraphPad Prism 5.0 software. Results: Trimetazidine (5 mg/kg and 10 mg/kg) significantly reduced MDA levels and increased SOD levels when compared with CCl4 treated group suggested antioxidant activity. Combined administration of Liv.52 and TMZ1 also reduced oxidative stress and increased antioxidant activity. Conclusion: Results of the present study suggested that increased oxidative stress was significantly attenuated by drug TMZ in dose dependant manner when compared with the CCl4 group. The antioxidant potential of prophylactic and therapeutic administration of TMZ was comparable. The increased antioxidant effect by Liv.52+TMZ1 combination was only due to the additive antioxidant effects of Liv.52 and TMZ or any other mechanism was involved, needs to be further evaluated.

Evaluation and correlation of oxidative stress and haemato-biochemical observations in horses with natural patent and latent trypanosomosis in Punjab state of India

2018 ◽  
Vol 63 (4) ◽  
pp. 733-743 ◽  
Author(s):  
Rahul Parashar ◽  
Lachhman Das Singla ◽  
Mayank Gupta ◽  
Suresh Kumar Sharma
Keyword(s):  
Oxidative Stress ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Latent Group ◽  
Biochemical Indices ◽  
And Oxidative Stress

Abstract The haemato-biochemical indices and oxidative stress markers in horses naturally infected with Trypanosoma evansi were evaluated by analyzing the level of these parameters between T. evansi infected (microscopically positive patent group and PCR positive latent group) and infection free horses. To compare the hemato-biochemical indices and oxidative stress indicators, horses were divided into three categories based on diagnostic test employed and positive results obtained. These included Romanowsky stained slide positive group (Group I; n = 6), PCR positive group (group II; n = 28) and negative control group (group III, n = 30), revealing parasitologically positive patent, molecular positive latent and disease free status of horses. A significant reductions in total erythrocytes count (TEC, P = 0.01), haemoglobin (Hb, P = 0.01) and packed cell volume (PCV, P = 0.04) was noticed both in group I and group II while significant neutrophilia and lymphocytopenia was observed in group I when compared to negative control group. Substantial increase in creatinine (CRTN, P = 0.032) and gamma glutamyl transferase (GGT, P = 0.012) in group I while significant decrease in glucose (GLU, P = 0.04) and iron (Fe, P = 0.01) were noticed in both group I and group II in comparison to group III. A significant difference in lipid peroxides (LPO, P = 0.01) with highest level in patent group I (15.33 ± 0.53) followed by PCR positive latent group (14.09 ± 1.66) indicates higher lipid peroxidation in erythrocytes and oxidative stress in decreasing order when compared with infection free control horses (9.83 ± 0.97). Catalase (CAT, P = 0.01) was significantly lower in parasitological (0.82 ± 0.14) and molecular positive cases (1.27 ± 0.35) in comparison to control group (3.43 ± 0.96). The levels of superoxide dismutase (SOD, P = 0.01), reduced glutathione (GSH, P = 0.01) and ferric reducing antioxidant power (FRAP, P = 0.01) were significantly lower in parasito-molecular positive cases as compared to infection free control horses. An inverse correlation of RBC count with LPO and GSH and a direct correlation with catalase, SOD and FRAP was revealed. Overall, the observed substantial decreases in the oxidative parameters like catalase CAT, SOD, GSH and FRAP activities with remarkably elevated levels of LPO indicate high exposure of erythrocytes to oxidative damage in T.evansi infected horses.

Thiamine deficiency and oxidative stress induced by prolonged metronidazole therapy can explain its side effects of neurotoxicity and infertility in experimental animals: Effect of grapefruit co-therapy

2020 ◽  
Vol 39 (6) ◽  
pp. 834-847
Author(s):  
MH Hassan ◽  
EA Awadalla ◽  
RA Ali ◽  
SS Fouad ◽  
E Abdel-Kahaar
Keyword(s):  
Oxidative Stress ◽  
Side Effects ◽  
Sperm Count ◽  
Free Testosterone ◽  
Testicular Tissue ◽  
Control Group ◽  
Group Iii ◽  
Stress Markers ◽  
Group I ◽  
Tissue Homogenates

We aimed to explore the possible neurotoxicity and infertility mechanisms of prolonged metronidazole (MTZ) use and the effects of antioxidant grapefruit (GP) co-therapy on MTZ-induced complications. Sixty male albino Wistar rats were divided into four groups ( n = 15 each). Group I (control group) received 1% dimethyl sulfoxide (27 ml/ kg/day), group II (MTZ group) received MTZ (400 mg/kg/day), group III (MTZ + GP) received MTZ (400 mg/kg/ day) plus GP juice (27 ml/kg/ day) and group IV (GP group) received GP juice (27 ml/kg) for 60 days. Semen analyses were performed. Free testosterone, gonadotrophin (follicle-stimulating hormone (FSH) and luteinizing hormone) and thiamine levels were measured. Samples of cerebellar, testicular and epididymal tissues were used for both colorimetric assays of oxidative stress markers and histopathological examinations. Significant decreases in the sperm count, percent total sperm motility, serum thiamine levels, free testosterone levels and FSH levels were observed in the MTZ and MTZ + GP groups ( p < 0.05 for all parameters). Significantly higher oxidative stress levels ( p < 0.05) were observed in the cerebellar and testicular tissue homogenates of these groups than in those of the control group, and associated disruptions in the cerebellar, testicular and epididymal structures were apparent compared to those of the control group. Although the GP group showed a significantly higher sperm count and significantly lower oxidative stress than the control group ( p < 0.05), with histological similarity to the control group, the GP group exhibited significantly higher prolactin levels and lower free testosterone and FSH levels than the control group ( p < 0.05). Oxidative stress and decreased thiamine levels could explain the MTZ-induced neurotoxicity and infertility side effects that aggravated by GP co-administration.

scholarly journals The Potential Protective Effect ofPhysalis peruvianaL. against Carbon Tetrachloride-Induced Hepatotoxicity in Rats Is Mediated by Suppression of Oxidative Stress and Downregulation of MMP-9 Expression

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Ebtisam M. Al-Olayan ◽  
Manal F. El-Khadragy ◽  
Ahmed M. Aref ◽  
Mohamed S. Othman ◽  
Rami B. Kassab ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Protective Effect ◽  
Inflammatory Marker ◽  
Control Group ◽  
Active Components ◽  
Active Constituent ◽  
Group Iii ◽  
Physalis Peruviana ◽  
Group I

The active constituent profile in Cape gooseberry (Physalis peruvianaL.) juice was determined by GC-MS. Quercetin and kaempferol were active components in the juice. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride (CCl4). Twenty-eight rats divided into 4 groups: Group I served as control group, and Group II received weekly i.p. injection of 2 mL CCl4/kg bwt for 12 weeks. Group III were supplemented with Physalis juice via the drinking water. The animals of Group IV received Physalis juice as Group III and also were intraperitoneally injected weekly with 2 mL CCl4/kg bwt for 12 weeks. Hepatoprotective effect was evaluated by improvement in liver enzymes serum levels, reduction in collagen areas, downregulation in expression of the fibrotic marker MMP-9, reduction in the peroxidative marker malonaldehyde and the inflammatory marker nitric oxide, and restoration of the activity of antioxidant enzymatic and nonenzymatic systems, namely, glutathione content, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities. The results show that the potential hepatoprotective effects ofPhysalis peruvianamay be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway.

scholarly journals The Impact of Endometritis on Specific Oxidative Stress Parameters in Cows

2018 ◽  
Vol 68 (2) ◽  
pp. 231
Author(s):  
S. KAYA ◽  
M. ÖĞÜN ◽  
H. ÖZEN ◽  
M. KURU ◽  
L. ŞAHIN ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Control Group ◽  
Group Iii ◽  
Uterine Infection ◽  
Oxidative Stress Parameters ◽  
Group I ◽  
Early Postpartum ◽  
Total Antioxidant ◽  
The Impact ◽  
Stress Parameters

This study was aimed to investigate the presence of uterine infection and the relationship between the severity of uterine infection and some oxidative stress parameters in cows in early postpartum period. Sixty six cows that were in the period between the 30th and 32rd days of postpartum were included in the study. Endometrial inflammation was scored according to the condition of the uterus at rectal and ultrasonographic examinations and the character and amount of uterine discharge from endometritis I through III, the latest being the most severe. Endometrial samples were taken from the clinically healthy animals by cytobrush method, and then stained with Giemsa for presence of polymorphonuclear leukocytes (PMN). Cows with a PMN percentage less than 18% were considered to be healthy (control, n=20). All cows were sampled for blood. Nitric oxide (NO) levels in Group I (Endometritis I; n=20), Group II (Endometritis II; n=16), Group III (Endometritis III; n=10) and the control group were determined to be 23.0±0.63 nmol/mL, 32.23±0.97 nmol/mL, 36.56±0.48 nmol/mL, and 11.10±0.29 nmol/mL, respectively. The differences among the groups were found to be statistically significant. Malondialdehyde (MDA) level was highest in Group III (6.38±0.13 μmol/L), and its level significantly decreased with the reduced severity of endometritis. The comparison of the groups for total antioxidant capacity (TAC) and total oxidant capacity (TOC) demonstrated that endometritis caused a decrease in TAC levels (P<0.05), but did not affect TOC levels. Endometritis increased serum NO and MDA levels, and decreased TAC levels, but had no effect on TOC levels. In conclusion, NO and MDA, as well as TAC can be used as a biochemical marker for estimation of the severity of endometritis in cows.

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